AJP - Lung Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 279: L143-L151, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (80)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, R.
Right arrow Articles by Uhal, B. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, R.
Right arrow Articles by Uhal, B. D.
Vol. 279, Issue 1, L143-L151, July 2000

Abrogation of bleomycin-induced epithelial apoptosis and lung fibrosis by captopril or by a caspase inhibitor

Rongqi Wang1, Olivia Ibarra-Sunga1, Luba Verlinski1, Ruth Pick1, and Bruce D. Uhal2

1 The Cardiovascular Institute and Division of Cardiology, Michael Reese Hospital, Chicago, Illinois 60616; and 2 Department of Physiology, Michigan State University, East Lansing, Michigan 48824

Angiotensin-converting enzyme is involved in apoptosis of alveolar epithelial cells (Wang R, Zagariya A, Ang E, Ibarra-Sunga O, and Uhal BD. Am J Physiol Lung Cell Mol Physiol 277: L1245-L1250, 1999). This study tested the ability of the angiotensin-converting enzyme inhibitor captopril or the caspase inhibitor Z-Val-Ala-Asp-fluoromethylketone (ZVAD-fmk) to block alveolar epithelial cell apoptosis and lung fibrosis in vivo in response to bleomycin (Bleo). Male Wistar rats received 8 U/kg of Bleo (bleomycin sulfate) or vehicle intratracheally. Subgroups of Bleo-treated rats received captopril, ZVAD-fmk, or vehicle alone. Lung collagen was assessed by picrosirius red or hydroxyproline assay at 1, 7, and 14 days post-Bleo, and apoptosis was detected by in situ end labeling (ISEL). Bleo increased alveolar septal and peribronchial collagen by 100 and 133%, respectively (both P < 0.01), by day 14 but not earlier. In contrast, ISEL was increased in alveolar and airway cells at all time points. Captopril or ZVAD-fmk inhibited collagen accumulation by 91 and 85%, respectively (P < 0.01). Both agents also inhibited ISEL in alveoli by 99 and 81% and in airways by 67 and 63%, respectively. These data suggest that the efficacy of captopril to inhibit experimental lung fibrogenesis is related to inhibition of apoptosis. They also demonstrate the antifibrotic potential of a caspase inhibitor.

interstitial pulmonary fibrosis; programmed cell death; fibrogenesis; type II pneumocytes; deoxyribonucleic acid fragmentation


This article has been cited by other articles:


Home page
 FASEB J.Home page
A. Abdul-Hafez, R. Shu, and B. D. Uhal
JunD and HIF-1{alpha} mediate transcriptional activation of angiotensinogen by TGF-{beta}1 in human lung fibroblasts
FASEB J, June 1, 2009; 23(6): 1655 - 1662.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
A G Richter, S McKeown, S Rathinam, L Harper, P Rajesh, D F McAuley, R Heljasvaara, and D R Thickett
Soluble endostatin is a novel inhibitor of epithelial repair in idiopathic pulmonary fibrosis
Thorax, February 1, 2009; 64(2): 156 - 161.
[Abstract] [Full Text] [PDF]

Home page
 ACCP Pulmonary Med Brd RevHome page
D. W. Kamp
Drug-Induced Lung Diseases
ACCP Pulmonary Med Brd Rev, January 1, 2009; 25(0): 319 - 344.
[Full Text] [PDF]

Home page
 ERRHome page
P. W. Noble
Epithelial fibroblast triggering and interactions in pulmonary fibrosis
Eur. Respir. Rev., December 1, 2008; 17(109): 123 - 129.
[Abstract] [Full Text] [PDF]

Home page
 ERRHome page
B. D. Uhal
The role of apoptosis in pulmonary fibrosis
Eur. Respir. Rev., December 1, 2008; 17(109): 138 - 144.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. Korfei, C. Ruppert, P. Mahavadi, I. Henneke, P. Markart, M. Koch, G. Lang, L. Fink, R.-M. Bohle, W. Seeger, et al.
Epithelial Endoplasmic Reticulum Stress and Apoptosis in Sporadic Idiopathic Pulmonary Fibrosis
Am. J. Respir. Crit. Care Med., October 15, 2008; 178(8): 838 - 846.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
G. J. Laurent, R. J. McAnulty, M. Hill, and R. Chambers
Escape from the Matrix: Multiple Mechanisms for Fibroblast Activation in Pulmonary Fibrosis
Proceedings of the ATS, April 15, 2008; 5(3): 311 - 315.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Konigshoff, A. Wilhelm, A. Jahn, D. Sedding, O. V. Amarie, B. Eul, W. Seeger, L. Fink, A. Gunther, O. Eickelberg, et al.
The Angiotensin II Receptor 2 Is Expressed and Mediates Angiotensin II Signaling in Lung Fibrosis
Am. J. Respir. Cell Mol. Biol., December 1, 2007; 37(6): 640 - 650.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Hammerschmidt, H. Kuhn, C. Gessner, H.-J. Seyfarth, and H. Wirtz
Stretch-Induced Alveolar Type II Cell Apoptosis: Role of Endogenous Bradykinin and PI3K-Akt Signaling
Am. J. Respir. Cell Mol. Biol., December 1, 2007; 37(6): 699 - 705.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
N. Pottier, C. Chupin, V. Defamie, B. Cardinaud, R. Sutherland, G. Rios, F. Gauthier, P. J. Wolters, Y. Berthiaume, P. Barbry, et al.
Relationships between Early Inflammatory Response to Bleomycin and Sensitivity to Lung Fibrosis: A Role for Dipeptidyl-Peptidase I and Tissue Inhibitor of Metalloproteinase-3?
Am. J. Respir. Crit. Care Med., December 1, 2007; 176(11): 1098 - 1107.
[Abstract] [Full Text] [PDF]

Home page
 Hum Exp ToxicolHome page
A. Elmi, Z. Sadeghi, S. Elmi, B. Daraei, and M. Ghazi-Khansari
Hepatoprotective role of captopril on paraquat induced hepatotoxicity
Human and Experimental Toxicology, October 1, 2007; 26(10): 789 - 794.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. M. Martin, J. A. Buckenberger, J. Jiang, G. E. Malana, D. L. Knoell, D. S. Feldman, and T. S. Elton
TGF-beta1 stimulates human AT1 receptor expression in lung fibroblasts by cross talk between the Smad, p38 MAPK, JNK, and PI3K signaling pathways
Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L790 - L799.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
N. J. Meyer and J. G. N. Garcia
Wading into the Genomic Pool to Unravel Acute Lung Injury Genetics
Proceedings of the ATS, January 1, 2007; 4(1): 69 - 76.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
X. Li, M. Molina-Molina, A. Abdul-Hafez, J. Ramirez, A. Serrano-Mollar, A. Xaubet, and B. D. Uhal
Extravascular sources of lung angiotensin peptide synthesis in idiopathic pulmonary fibrosis
Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L887 - L895.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
T. R. Martin, N. Hagimoto, M. Nakamura, and G. Matute-Bello
Apoptosis and Epithelial Injury in the Lungs
Proceedings of the ATS, October 1, 2005; 2(3): 214 - 220.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. Y. Lee, C. Schroedl, J. K. Brunelle, L. J. Buccellato, O. I. Akinci, H. Kaneto, C. Snyder, J. Eisenbart, G. R. S. Budinger, and N. S. Chandel
Bleomycin induces alveolar epithelial cell death through JNK-dependent activation of the mitochondrial death pathway
Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L521 - L528.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Wang, L. Bowman, Y. Lu, Y. Rojanasakul, R. R. Mercer, V. Castranova, and M. Ding
Essential role of p53 in silica-induced apoptosis
Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L488 - L496.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
H Miyazaki, K Kuwano, K Yoshida, T Maeyama, M Yoshimi, M Fujita, N Hagimoto, R Yoshida, and Y Nakanishi
The perforin mediated apoptotic pathway in lung injury and fibrosis
J. Clin. Pathol., December 1, 2004; 57(12): 1292 - 1298.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
X. Li, R. Shu, G. Filippatos, and B. D. Uhal
Apoptosis in lung injury and remodeling
J Appl Physiol, October 1, 2004; 97(4): 1535 - 1542.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
H. F. Nadrous, J. H. Ryu, W. W. Douglas, P. A. Decker, and E. J. Olson
Impact of Angiotensin-Converting Enzyme Inhibitors and Statins on Survival in Idiopathic Pulmonary Fibrosis
Chest, August 1, 2004; 126(2): 438 - 446.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Geiser, M. Ishigaki, C. van Leer, M. A. Matthay, and V. C. Broaddus
H2O2 inhibits alveolar epithelial wound repair in vitro by induction of apoptosis
Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L448 - L453.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Inoshima, K. Kuwano, N. Hamada, M. Yoshimi, T. Maeyama, N. Hagimoto, Y. Nakanishi, and N. Hara
Induction of CDK inhibitor p21 gene as a new therapeutic strategy against pulmonary fibrosis
Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L727 - L733.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
M Otsuka, H Takahashi, M Shiratori, H Chiba, and S Abe
Reduction of bleomycin induced lung fibrosis by candesartan cilexetil, an angiotensin II type 1 receptor antagonist
Thorax, January 1, 2004; 59(1): 31 - 38.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. P. Marshall, P. Gohlke, R. C. Chambers, D. C. Howell, S. E. Bottoms, T. Unger, R. J. McAnulty, and G. J. Laurent
Angiotensin II and the fibroproliferative response to acute lung injury
Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L156 - L164.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Fujita, J. M. Shannon, O. Morikawa, J. Gauldie, N. Hara, and R. J. Mason
Overexpression of Tumor Necrosis Factor-{alpha} Diminishes Pulmonary Fibrosis Induced by Bleomycin or Transforming Growth Factor-{beta}
Am. J. Respir. Cell Mol. Biol., December 1, 2003; 29(6): 669 - 676.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
N. Kaminski, J. A. Belperio, P. B. Bitterman, L. Chen, S. W. Chensue, A. M.K. Choi, S. Dacic, J. H. Dauber, R. M. du Bois, J. J. Enghild, et al.
Idiopathic Pulmonary Fibrosis
Am. J. Respir. Cell Mol. Biol., September 1, 2003; 29(3): S1 - 105.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
X. Li, H. Zhang, V. Soledad-Conrad, J. Zhuang, and B. D. Uhal
Bleomycin-induced apoptosis of alveolar epithelial cells requires angiotensin synthesis de novo
Am J Physiol Lung Cell Mol Physiol, March 1, 2003; 284(3): L501 - L507.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. Ghosh, T. Mendoza, L. A. Ortiz, G. W. Hoyle, C. D. Fermin, A. R. Brody, M. Friedman, and G. F. Morris
Bleomycin Sensitivity of Mice Expressing Dominant-Negative p53 in the Lung Epithelium
Am. J. Respir. Crit. Care Med., September 15, 2002; 166(6): 890 - 897.
[Abstract] [Full Text]

Home page
 Hum Exp ToxicolHome page
F Candan and H Alagozlu
Captopril inhibits the pulmonary toxicity of paraquat in rats
Human and Experimental Toxicology, December 1, 2001; 20(12): 637 - 641.
[Abstract] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. M. Day, Y. Yang, Y. J. Suzuki, J. Stevens, R. Pathi, A. Perlmutter, B. L. Fanburg, and J. J. Lanzillo
Bleomycin Upregulates Gene Expression of Angiotensin-Converting Enzyme via Mitogen-Activated Protein Kinase and Early Growth Response 1 Transcription Factor
Am. J. Respir. Cell Mol. Biol., November 1, 2001; 25(5): 613 - 619.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. S. Filippatos, N. Gangopadhyay, O. Lalude, N. Parameswaran, S. I. Said, W. Spielman, and B. D. Uhal
Regulation of apoptosis by vasoactive peptides
Am J Physiol Lung Cell Mol Physiol, October 1, 2001; 281(4): L749 - L761.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. E. Dincer, N. Gangopadhyay, R. Wang, and B. D. Uhal
Norepinephrine induces alveolar epithelial apoptosis mediated by {alpha}-, {beta}-, and angiotensin receptor activation
Am J Physiol Lung Cell Mol Physiol, September 1, 2001; 281(3): L624 - L630.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
O. EICKELBERG, A. PANSKY, E. KOEHLER, M. BIHL, M. TAMM, P. HILDEBRAND, A. P. PERRUCHOUD, M. KASHGARIAN, and M. ROTH
Molecular mechanisms of TGF-{beta} antagonism by interferon {gamma} and cyclosporine A in lung fibroblasts
FASEB J, March 1, 2001; 15(3): 797 - 806.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Fine, Y. Janssen-Heininger, R. P. Soultanakis, S. G. Swisher, and B. D. Uhal
Apoptosis in lung pathophysiology
Am J Physiol Lung Cell Mol Physiol, September 1, 2000; 279(3): L423 - L427.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online