AJP - Lung Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 279: L216-L223, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weikert, L. F.
Right arrow Articles by Shepherd, V. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weikert, L. F.
Right arrow Articles by Shepherd, V. L.
Vol. 279, Issue 2, L216-L223, August 2000

Surfactant protein A enhances mycobacterial killing by rat macrophages through a nitric oxide-dependent pathway

Laura F. Weikert1, Joseph P. Lopez2, Rasul Abdolrasulnia1, Zissis C. Chroneos3, and Virginia L. Shepherd2,4

Departments of 1 Medicine and 2 Pathology, Vanderbilt University School of Medicine, and 4 Veterans Affairs Medical Center, Nashville, Tennessee 37212; and 3 Children's Hospital Medical Center, Cincinnati, Ohio 45229

Surfactant-associated protein A (SP-A) is involved in surfactant homeostasis and host defense in the lung. We have previously demonstrated that SP-A specifically binds to and enhances the ingestion of bacillus Calmette-Guerin (BCG) organisms by macrophages. In the current study, we investigated the effect of SP-A on the generation of inflammatory mediators induced by BCG and the subsequent fate of ingested BCG organisms. Rat macrophages were incubated with BCG in the presence and absence of SP-A. Noningested BCG organisms were removed, and the release of tumor necrosis factor-alpha (TNF-alpha ) and nitric oxide were measured at varying times. TNF-alpha and nitric oxide production induced by BCG were enhanced by SP-A. In addition, SP-A enhanced the BCG-induced increase in the level of inducible nitric oxide synthase protein. Addition of antibodies directed against SPR210, a specific macrophage SP-A receptor, inhibited the SP-A-enhanced mediator production. BCG in the absence of SP-A showed increased growth over a 5-day period, whereas inclusion of SP-A dramatically inhibited BCG growth. Inhibition of nitric oxide production blocked BCG killing in the presence and absence of SP-A. These results demonstrate that ingestion of SP-A-BCG complexes by rat macrophages leads to production of inflammatory mediators and increased mycobacterial killing.

phagocytosis; mycobacteria; surfactant-associated protein A


This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
B. Samten, J. C. Townsend, Z. Sever-Chroneos, V. Pasquinelli, P. F. Barnes, and Z. C. Chroneos
An antibody against the surfactant protein A (SP-A)-binding domain of the SP-A receptor inhibits T cell-mediated immune responses to Mycobacterium tuberculosis
J. Leukoc. Biol., July 1, 2008; 84(1): 115 - 123.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. E. Oberley, K. A. Ault, T. L. Neff, K. R. Khubchandani, E. C. Crouch, and J. M. Snyder
Surfactant proteins A and D enhance the phagocytosis of Chlamydia into THP-1 cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L296 - L306.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. A. Gold, Y. Hoshino, N. Tanaka, W. N. Rom, B. Raju, R. Condos, and M. D. Weiden
Surfactant Protein A Modulates the Inflammatory Response in Macrophages during Tuberculosis
Infect. Immun., February 1, 2004; 72(2): 645 - 650.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
J. P. Lopez, E. Clark, and V. L. Shepherd
Surfactant protein A enhances Mycobacterium avium ingestion but not killing by rat macrophages
J. Leukoc. Biol., October 1, 2003; 74(4): 523 - 530.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Hussain, J. R. Wright, and W. J. Martin II
Surfactant Protein A Decreases Nitric Oxide Production by Macrophages in a Tumor Necrosis Factor-{alpha}-Dependent Mechanism
Am. J. Respir. Cell Mol. Biol., April 1, 2003; 28(4): 520 - 527.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Chabot, L. Salez, F. X. McCormack, L. Touqui, and M. Chignard
Surfactant Protein A Inhibits Lipopolysaccharide-Induced In Vivo Production of Interleukin-10 by Mononuclear Phagocytes during Lung Inflammation
Am. J. Respir. Cell Mol. Biol., March 1, 2003; 28(3): 347 - 353.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. L. Shepherd
Pulmonary surfactant protein D: a novel link between innate and adaptive immunity
Am J Physiol Lung Cell Mol Physiol, March 1, 2002; 282(3): L516 - L517.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
J. S. Ferguson, D. R. Voelker, J. A. Ufnar, A. J. Dawson, and L. S. Schlesinger
Surfactant Protein D Inhibition of Human Macrophage Uptake of Mycobacterium tuberculosis Is Independent of Bacterial Agglutination
J. Immunol., February 1, 2002; 168(3): 1309 - 1314.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
T. R. Korfhagen
Surfactant Protein A (SP-A)-Mediated Bacterial Clearance . SP-A and Cystic Fibrosis
Am. J. Respir. Cell Mol. Biol., December 1, 2001; 25(6): 668 - 672.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. M. Hickman-Davis, F. C. Fang, C. Nathan, V. L. Shepherd, D. R. Voelker, and J. R. Wright
Lung surfactant and reactive oxygen-nitrogen species: antimicrobial activity and host-pathogen interactions
Am J Physiol Lung Cell Mol Physiol, September 1, 2001; 281(3): L517 - L523.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. R. Wright, P. Borron, K. G. Brinker, and R. J. Folz
Surfactant Protein A . Regulation of Innate and Adaptive Immune Responses in Lung Inflammation
Am. J. Respir. Cell Mol. Biol., May 1, 2001; 24(5): 513 - 517.
[Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Zhao, S. S. Chua, M. M. Burcin, S. D. Reynolds, B. R. Stripp, R. A. Edwards, M. J. Finegold, S. Y. Tsai, and F. J. DeMayo
Phenotypic consequences of lung-specific inducible expression of FGF-3
PNAS, May 8, 2001; 98(10): 5898 - 5903.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online