AJP - Lung AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 279: L262-L272, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (36)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sham, J. S. K.
Right arrow Articles by Sylvester, J. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sham, J. S. K.
Right arrow Articles by Sylvester, J. T.
Vol. 279, Issue 2, L262-L272, August 2000

Effects of hypoxia in porcine pulmonary arterial myocytes: roles of KV channel and endothelin-1

James S. K. Sham, Benjamin R. Crenshaw Jr., Li-Hua Deng, Larissa A. Shimoda, and J. T. Sylvester

Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21224

Effects of acute hypoxia on intracellular Ca2+ concentration ([Ca2+]i) and cell length were recorded simultaneously in proximal and distal pulmonary (PASMCs) and femoral (FASMCs) arterial smooth muscle cells. Reducing PO2 from normoxia to severe hypoxia (PO2 < 10 mmHg) caused small but significant decreases in length and a reversible increase in [Ca2+]i in distal PASMCs and a small decrease in length in proximal PASMCs but had no effect in FASMCs, even though all three cell types contracted significantly to vasoactive agonists. Inhibition of voltage-dependent K+ (KV) channel with 4-aminopyridine produced a greater increase in [Ca2+]i in distal than in proximal PASMCs. In distal PASMCs, severe hypoxia caused a slight inhibition of KV currents; however, it elicited further contraction in the presence of 4-aminopyridine. Endothelin-1 (10-10 M), which itself did not alter cell length or [Ca2+]i, significantly potentiated the hypoxic contraction. These results suggest that hypoxia only has small direct effects on porcine PASMCs. These effects cannot be fully explained by inhibition of KV channels and were greatly enhanced via synergistic interactions with the endothelium-derived factor endothelin-1.

vasoconstriction; calcium; voltage-dependent potassium channel; pulmonary arterial smooth muscle cell


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. Lu, J. Wang, L. A. Shimoda, and J. T. Sylvester
Differences in STIM1 and TRPC expression in proximal and distal pulmonary arterial smooth muscle are associated with differences in Ca2+ responses to hypoxia
Am J Physiol Lung Cell Mol Physiol, July 1, 2008; 295(1): L104 - L113.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. M. Whitman, S. Pisarcik, T. Luke, M. Fallon, J. Wang, J. T. Sylvester, G. L. Semenza, and L. A. Shimoda
Endothelin-1 mediates hypoxia-induced inhibition of voltage-gated K+ channel expression in pulmonary arterial myocytes
Am J Physiol Lung Cell Mol Physiol, February 1, 2008; 294(2): L309 - L318.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Fantozzi, O. Platoshyn, A. H. Wong, S. Zhang, C. V. Remillard, M. R. Furtado, O. V. Petrauskene, and J. X.-J. Yuan
Bone morphogenetic protein-2 upregulates expression and function of voltage-gated K+ channels in human pulmonary artery smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L993 - L1004.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. P. T. Ward, T. P. Robertson, and P. I. Aaronson
Capacitative calcium entry: a central role in hypoxic pulmonary vasoconstriction?
Am J Physiol Lung Cell Mol Physiol, July 1, 2005; 289(1): L2 - L4.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Weigand, J. Foxson, J. Wang, L. A. Shimoda, and J. T. Sylvester
Inhibition of hypoxic pulmonary vasoconstriction by antagonists of store-operated Ca2+ and nonselective cation channels
Am J Physiol Lung Cell Mol Physiol, July 1, 2005; 289(1): L5 - L13.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Wang, L. A. Shimoda, L. Weigand, W. Wang, D. Sun, and J. T. Sylvester
Acute hypoxia increases intracellular [Ca2+] in pulmonary arterial smooth muscle by enhancing capacitative Ca2+ entry
Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1059 - L1069.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. B. Waypa and P. T. Schumacker
Hypoxic pulmonary vasoconstriction: redox events in oxygen sensing
J Appl Physiol, January 1, 2005; 98(1): 404 - 414.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. Hohne, M. O. Krebs, M. Seiferheld, W. Boemke, G. Kaczmarczyk, and E. R. Swenson
Acetazolamide prevents hypoxic pulmonary vasoconstriction in conscious dogs
J Appl Physiol, August 1, 2004; 97(2): 515 - 521.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
F. Zhang, J. I. Kaide, L. Yang, H. Jiang, S. Quan, R. Kemp, W. Gong, M. Balazy, N. G. Abraham, and A. Nasjletti
CO modulates pulmonary vascular response to acute hypoxia: relation to endothelin
Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H137 - H144.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W.-M. Zhang, K.-P. Yip, M.-J. Lin, L. A. Shimoda, W.-H. Li, and J. S. K. Sham
ET-1 activates Ca2+ sparks in PASMC: local Ca2+ signaling between inositol trisphosphate and ryanodine receptors
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L680 - L690.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Q. Liu, J. S. K. Sham, L. A. Shimoda, P. Kuppusamy, and J. T. Sylvester
Hypoxic constriction and reactive oxygen species in porcine distal pulmonary arteries
Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L322 - L333.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. P. Robertson, P. I. Aaronson, and J. P. T. Ward
Ca2+ sensitization during sustained hypoxic pulmonary vasoconstriction is endothelium dependent
Am J Physiol Lung Cell Mol Physiol, June 1, 2003; 284(6): L1121 - L1126.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Olschewski, Z. Hong, D. P. Nelson, and E. K. Weir
Graded response of K+ current, membrane potential, and [Ca2+]i to hypoxia in pulmonary arterial smooth muscle
Am J Physiol Lung Cell Mol Physiol, November 1, 2002; 283(5): L1143 - L1150.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J. S.K. Sham
Hypoxic Pulmonary Vasoconstriction: Ups and Downs of Reactive Oxygen Species
Circ. Res., October 18, 2002; 91(8): 649 - 651.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. V. Remillard, W.-M. Zhang, L. A. Shimoda, and J. S. K. Sham
Physiological properties and functions of Ca2+ sparks in rat intrapulmonary arterial smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L433 - L444.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. A. Coppock and M. M. Tamkun
Differential expression of KV channel alpha - and beta -subunits in the bovine pulmonary arterial circulation
Am J Physiol Lung Cell Mol Physiol, December 1, 2001; 281(6): L1350 - L1360.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. Weissmann, F. Grimminger, A. Olschewski, and W. Seeger
Hypoxic pulmonary vasoconstriction: a multifactorial response?
Am J Physiol Lung Cell Mol Physiol, August 1, 2001; 281(2): L314 - L317.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. A. Coppock, J. R. Martens, and M. M. Tamkun
Molecular basis of hypoxia-induced pulmonary vasoconstriction: role of voltage-gated K+ channels
Am J Physiol Lung Cell Mol Physiol, July 1, 2001; 281(1): L1 - L12.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J. T. Sylvester
Hypoxic Pulmonary Vasoconstriction : A Radical View
Circ. Res., June 22, 2001; 88(12): 1228 - 1230.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. A. Madden
Focus on "Hypoxic constriction of porcine distal pulmonary arteries: endothelium and endothelin dependence"
Am J Physiol Lung Cell Mol Physiol, May 1, 2001; 280(5): L853 - L855.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Q. Liu, J. S. K. Sham, L. A. Shimoda, and J. T. Sylvester
Hypoxic constriction of porcine distal pulmonary arteries: endothelium and endothelin dependence
Am J Physiol Lung Cell Mol Physiol, May 1, 2001; 280(5): L856 - L865.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
G. B. Waypa, N. S. Chandel, and P. T. Schumacker
Model for Hypoxic Pulmonary Vasoconstriction Involving Mitochondrial Oxygen Sensing
Circ. Res., June 22, 2001; 88(12): 1259 - 1266.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online