AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 279: L302-L311, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (61)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by True, A. L.
Right arrow Articles by Malik, A. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by True, A. L.
Right arrow Articles by Malik, A. B.
Vol. 279, Issue 2, L302-L311, August 2000

Activation of NF-kappa B induced by H2O2 and TNF-alpha and its effects on ICAM-1 expression in endothelial cells

Andrea L. True, Arshad Rahman, and Asrar B. Malik

Department of Pharmacology, University of Illinois College of Medicine, Chicago, Illinois 60612

Reactive oxygen species have been proposed to signal the activation of the transcription factor nuclear factor (NF)-kappa B in response to tumor necrosis factor (TNF)-alpha challenge. In the present study, we investigated the effects of H2O2 and TNF-alpha in mediating activation of NF-kappa B and transcription of the intercellular adhesion molecule (ICAM)-1 gene. Northern blot analysis showed that TNF-alpha exposure of human dermal microvascular endothelial cells (HMEC-1) induced marked increases in ICAM-1 mRNA and cell surface protein expression. In contrast, H2O2 added at subcytolytic concentrations failed to activate ICAM-1 expression. Challenge with H2O2 also failed to induce NF-kappa B-driven reporter gene expression in the transduced HMEC-1 cells, whereas TNF-alpha increased the NF-kappa B-driven gene expression ~10-fold. Gel supershift assay revealed the presence of p65 (Rel A), p50, and c-Rel in both H2O2- and TNF-alpha -induced NF-kappa B complexes bound to the ICAM-1 promoter, with the binding of the p65 subunit being the most prominent. In vivo phosphorylation studies, however, showed that TNF-alpha exposure induced marked phosphorylation of NF-kappa B p65 in HMEC-1 cells, whereas H2O2 had no effect. These results suggest that reactive oxygen species generation in endothelial cells mediates the binding of NF-kappa B to nuclear DNA, whereas TNF-alpha generates additional signals that induce phosphorylation of the bound NF-kappa B p65 and confer transcriptional competency to NF-kappa B.

redox state; intercellular adhesion molecule-1 promoter; tumor necrosis factor-alpha ; oxidants; nuclear factor-kappa B; signaling; hydrogen peroxide


This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
J. W. Zmijewski, E. Lorne, X. Zhao, Y. Tsuruta, Y. Sha, G. Liu, G. P. Siegal, and E. Abraham
Mitochondrial Respiratory Complex I Regulates Neutrophil Activation and Severity of Lung Injury
Am. J. Respir. Crit. Care Med., July 15, 2008; 178(2): 168 - 179.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. W. Zmijewski, X. Zhao, Z. Xu, and E. Abraham
Exposure to hydrogen peroxide diminishes NF-{kappa}B activation, I{kappa}B-{alpha} degradation, and proteasome activity in neutrophils
Am J Physiol Cell Physiol, July 1, 2007; 293(1): C255 - C266.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
S. Y. Eum, G. B. Rha, B. Hennig, and M. Toborek
c-Src Is the Primary Signaling Mediator of Polychlorinated Biphenyl-Induced Interleukin-8 Expression in a Human Microvascular Endothelial Cell Line
Toxicol. Sci., July 1, 2006; 92(1): 311 - 320.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
S. Y. Eum, Y. W. Lee, B. Hennig, and M. Toborek
Interplay between Epidermal Growth Factor Receptor and Janus Kinase 3 Regulates Polychlorinated Biphenyl-Induced Matrix Metalloproteinase-3 Expression and Transendothelial Migration of Tumor Cells
Mol. Cancer Res., June 1, 2006; 4(6): 361 - 370.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
N. Khan, S. S. Rahim, C. S. Boddupalli, S. Ghousunnissa, S. Padma, N. Pathak, D. Thiagarajan, S. E. Hasnain, and S. Mukhopadhyay
Hydrogen peroxide inhibits IL-12 p40 induction in macrophages by inhibiting c-rel translocation to the nucleus through activation of calmodulin protein
Blood, February 15, 2006; 107(4): 1513 - 1520.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. Mehta and A. B. Malik
Signaling Mechanisms Regulating Endothelial Permeability
Physiol Rev, January 1, 2006; 86(1): 279 - 367.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
H. Cai
Hydrogen peroxide regulation of endothelial function: Origins, mechanisms, and consequences
Cardiovasc Res, October 1, 2005; 68(1): 26 - 36.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. C. Villar, H. Kashleva, A. P. Mitchell, and A. Dongari-Bagtzoglou
Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection
Infect. Immun., August 1, 2005; 73(8): 4588 - 4595.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
Z. Biao, X. Zhanggang, J. Hao, M. Changhong, and C. Jing
The In Vitro Effect of Desflurane Preconditioning on Endothelial Adhesion Molecules and mRNA Expression
Anesth. Analg., April 1, 2005; 100(4): 1007 - 1013.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J.-M. Li and A. M Shah
Endothelial cell superoxide generation: regulation and relevance for cardiovascular pathophysiology
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2004; 287(5): R1014 - R1030.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Strassheim, K. Asehnoune, J.-S. Park, J.-Y. Kim, Q. He, D. Richter, S. Mitra, J. Arcaroli, K. Kuhn, and E. Abraham
Modulation of bone marrow-derived neutrophil signaling by H2O2: disparate effects on kinases, NF-{kappa}B, and cytokine expression
Am J Physiol Cell Physiol, March 1, 2004; 286(3): C683 - C692.
[Abstract] [Full Text]

Home page
 FASEB J.Home page
W.-J. ZHANG and B. FREI
{alpha}-Lipoic acid inhibits TNF-{alpha}-induced NF-{kappa}B activation and adhesion molecule expression in human aortic endothelial cells
FASEB J, November 1, 2001; 15(13): 2423 - 2432.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Rahman, K. N. Anwar, S. Uddin, N. Xu, R. D. Ye, L. C. Platanias, and A. B. Malik
Protein Kinase C-{delta} Regulates Thrombin-Induced ICAM-1 Gene Expression in Endothelial Cells via Activation of p38 Mitogen-Activated Protein Kinase
Mol. Cell. Biol., August 15, 2001; 21(16): 5554 - 5565.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
I. Jaspers, W. Zhang, A. Fraser, J. M. Samet, and W. Reed
Hydrogen Peroxide Has Opposing Effects on IKK Activity and Ikappa Balpha Breakdown in Airway Epithelial Cells
Am. J. Respir. Cell Mol. Biol., June 1, 2001; 24(6): 769 - 777.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Dai and A. Churg
Relationship of Fiber Surface Iron and Active Oxygen Species to Expression of Procollagen, PDGF-A, and TGF-{beta}1 in Tracheal Explants Exposed to Amosite Asbestos
Am. J. Respir. Cell Mol. Biol., April 1, 2001; 24(4): 427 - 435.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Lum and K. A. Roebuck
Oxidant stress and endothelial cell dysfunction
Am J Physiol Cell Physiol, April 1, 2001; 280(4): C719 - C741.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Ischiropoulos
Reactive species and signal transduction
Am J Physiol Lung Cell Mol Physiol, April 1, 2001; 280(4): L583 - L584.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online