AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 279: L319-L325, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hotta, K.
Right arrow Articles by Emala, C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hotta, K.
Right arrow Articles by Emala, C. W.
Vol. 279, Issue 2, L319-L325, August 2000

TNF-alpha increases transcription of Galpha i-2 in human airway smooth muscle cells

Kunihisa Hotta, Carol A. Hirshman, and Charles W. Emala

Department of Anesthesiology, College of Physicians and Surgeons of Columbia University, New York, New York 10032

Tumor necrosis factor-alpha (TNF-alpha ) is a proinflammatory cytokine that has an important role in the regulation of airway smooth muscle tone and reactivity. We have shown previously that TNF-alpha upregulates the expression of Galpha i-2 protein without significantly increasing Gsalpha protein and enhances adenylyl cyclase inhibition by carbachol in cultured human airway smooth muscle cells (Hotta K, Emala CW, and Hirshman CA. Am J Physiol Lung Cell Mol Physiol 276: L405-L411, 1999). The present study was designed to investigate the molecular mechanisms by which TNF-alpha upregulates Galpha i-2 protein in these cells. TNF-alpha pretreatment for 48 h increased the expression of Galpha i-2 protein without significantly altering the Galpha i-2 protein half-life (41.0 ± 8.2 h for control and 46.8 ± 5.2 h for TNF-alpha -treated cells). Inhibition of new protein synthesis by cycloheximide blocked the increase in Galpha i-2 protein induced by TNF-alpha . Furthermore, TNF-alpha treatment for 12-24 h increased the steady-state level of Galpha i-2 mRNA without significantly altering Galpha i-2 mRNA half-life (9.0 ± 0.75 h for control and 8.9 ± 1.1 h for TNF-alpha -treated cells). The transcription inhibitor actinomycin D blocked the increase in Galpha i-2 mRNA induced by TNF-alpha . These observations indicate that the increase in Galpha i-2 protein induced by TNF-alpha is due to an increased rate of Galpha i-2 protein synthesis, most likely as a consequence of the transcriptional increase in the steady-state levels of its mRNA.

trachea; G protein; messenger ribonucleic acid half-life; protein half-life; antinomycin D; cycloheximide


This article has been cited by other articles:


Home page
 ThoraxHome page
P H Howarth, K S Babu, H S Arshad, L Lau, M Buckley, W McConnell, P Beckett, M Al Ali, A Chauhan, S J Wilson, et al.
Tumour necrosis factor (TNF{alpha}) as a novel therapeutic target in symptomatic corticosteroid dependent asthma
Thorax, December 1, 2005; 60(12): 1012 - 1018.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. A. Deshpande, T. A. White, S. Dogan, T. F. Walseth, R. A. Panettieri, and M. S. Kannan
CD38/cyclic ADP-ribose signaling: role in the regulation of calcium homeostasis in airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L773 - L788.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
I. Hunter, H. J. Cobban, P. Vandenabeele, D. J. MacEwan, and G. F. Nixon
Tumor Necrosis Factor-alpha -Induced Activation of RhoA in Airway Smooth Muscle Cells: Role in the Ca2+ Sensitization of Myosin Light Chain20 Phosphorylation
Mol. Pharmacol., March 1, 2003; 63(3): 714 - 721.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. J. Janssen
Ionic mechanisms and Ca2+ regulation in airway smooth muscle contraction: do the data contradict dogma?
Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1161 - L1178.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online