AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 279: L390-L398, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wheeler, M. D.
Right arrow Articles by Thurman, R. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wheeler, M. D.
Right arrow Articles by Thurman, R. G.
Vol. 279, Issue 2, L390-L398, August 2000

Dietary glycine blunts lung inflammatory cell influx following acute endotoxin

Michael D. Wheeler, Michelle L. Rose, Shunhei Yamashima, Nobuyuki Enomoto, Vitor Seabra, Jonathan Madren, and Ronald G. Thurman

Laboratory of Hepatobiology and Toxicology, Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina 27599-7365

Mortality associated with endotoxin shock is likely mediated by Kupffer cells, alveolar macrophages, and circulating neutrophils. Acute dietary glycine prevents mortality and blunts increases in serum tumor necrosis factor-alpha (TNF-alpha ) following endotoxin in rats. Furthermore, acute glycine blunts activation of Kupffer cells, alveolar macrophages, and neutrophils by activating a glycine-gated chloride channel. However, in neuronal tissue, glycine rapidly downregulates chloride channel function. Therefore, the long-term effects of a glycine-containing diet on survival following endotoxin shock were investigated. Dietary glycine for 4 wk improved survival after endotoxin but did not improve liver pathology, decrease serum alanine transaminase, or effect TNF-alpha levels compared with animals fed control diet. Interestingly, dietary glycine largely prevented inflammation and injury in the lung following endotoxin. Surprisingly, Kupffer cells from animals fed glycine for 4 wk were no longer inactivated by glycine in vitro; however, isolated alveolar macrophages and neutrophils from the same animals were sensitive to glycine. These data are consistent with the hypothesis that glycine downregulates chloride channels on Kupffer cells but not on alveolar macrophages or neutrophils. Importantly, glycine diet for 4 wk protected against lung inflammation due to endotoxin. Chronic glycine improves survival by unknown mechanisms, but reduction of lung inflammation is likely involved.

endotoxin shock; Kupffer cells; alveolar macrophages; neutrophils; tumor necrosis factor-alpha


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. E. Hafidi, I. Perez, J. Zamora, V. Soto, G. Carvajal-Sandoval, and G. Banos
Glycine intake decreases plasma free fatty acids, adipose cell size, and blood pressure in sucrose-fed rats
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2004; 287(6): R1387 - R1393.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
P. A. Verhoef, S. B. Kertesy, M. Estacion, W. P. Schilling, and G. R. Dubyak
Maitotoxin Induces Biphasic Interleukin-1{beta} Secretion and Membrane Blebbing in Murine Macrophages
Mol. Pharmacol., October 1, 2004; 66(4): 909 - 920.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Froh, R. G. Thurman, and M. D. Wheeler
Molecular evidence for a glycine-gated chloride channel in macrophages and leukocytes
Am J Physiol Gastrointest Liver Physiol, October 1, 2002; 283(4): G856 - G863.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online