AJP - Lung Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 279: L863-L871, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, C.
Right arrow Articles by Nielsen, H. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, C.
Right arrow Articles by Nielsen, H. C.
Vol. 279, Issue 5, L863-L871, November 2000

Hoxa-5 in mouse developing lung: cell-specific expression and retinoic acid regulation

Chiwan Kim and Heber C. Nielsen

Division of Newborn Medicine, Floating Hospital for Children at New England Medical Center and Tufts School of Medicine, Boston, Massachusetts 02111

Hoxa-5 is a homeobox gene that is highly expressed in the developing mouse lung. However, little is known about the molecular mechanisms controlling expression. We characterized the ontogeny of Hoxa-5 gene and protein expressions during lung development and then studied the cell-specific effects of retinoic acid (RA) on Hoxa-5 mRNA in fetal lung fibroblasts and MLE-12 mouse lung epithelial cells. Strong but constant Hoxa-5 gene and protein expressions were detected from mouse lung on embryonic day 13.5 to postnatal day 2. At baseline, the gene was strongly expressed in the fibroblasts of day 17.5 fetal mouse lungs. A very weak but reproducible expression was present in the MLE-12 cells. RA stimulated gene expression in both cell types in a time- and dose-dependent manner. Peak expression occurred much later in the MLE-12 cells compared with that in fibroblasts. Cycloheximide and actinomycin D treatment studies suggested that the differences in RA effect on each cell type may involve the presence of a repressor that can be overcome by RA.

homeobox genes; fetal lung


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. V. Volpe, E. Chung, J. P. Ulm, B. F. Gilchrist, S. Ralston, K. T. Wang, and H. C. Nielsen
Aberrant cell adhesion molecule expression in human bronchopulmonary sequestration and congenital cystic adenomatoid malformation
Am J Physiol Lung Cell Mol Physiol, July 1, 2009; 297(1): L143 - L152.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
H. Chen, H. Zhang, J. Lee, X. Liang, X. Wu, T. Zhu, P.-k. Lo, X. Zhang, and S. Sukumar
HOXA5 Acts Directly Downstream of Retinoic Acid Receptor {beta} and Contributes to Retinoic Acid Induced Apoptosis and Growth Inhibition
Cancer Res., September 1, 2007; 67(17): 8007 - 8013.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online