AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 279: L884-L894, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (36)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shimoda, L. A.
Right arrow Articles by Sylvester, J. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shimoda, L. A.
Right arrow Articles by Sylvester, J. T.
Vol. 279, Issue 5, L884-L894, November 2000

L-type Ca2+ channels, resting [Ca2+]i, and ET-1-induced responses in chronically hypoxic pulmonary myocytes

Larissa A. Shimoda, James S. K. Sham, Tenille H. Shimoda, and J. T. Sylvester

Division of Pulmonary and Critical Care Medicine, Department of Medicine, The Johns Hopkins University, Baltimore, Maryland 21224

In the lung, chronic hypoxia (CH) causes pulmonary arterial smooth muscle cell (PASMC) depolarization, elevated endothelin-1 (ET-1), and vasoconstriction. We determined whether, during CH, depolarization-driven activation of L-type Ca2+ channels contributes to 1) maintenance of resting intracellular Ca2+ concentration ([Ca2+]i), 2) increased [Ca2+]i in response to ET-1 (10-8 M), and 3) ET-1-induced contraction. Using indo 1 microfluorescence, we determined that resting [Ca2+]i in PASMCs from intrapulmonary arteries of rats exposed to 10% O2 for 21 days was 293.9 ± 25.2 nM (vs. 153.6 ± 28.7 nM in normoxia). Resting [Ca2+]i was decreased after extracellular Ca2+ removal but not with nifedipine (10-6 M), an L-type Ca2+ channel antagonist. After CH, the ET-1-induced increase in [Ca2+]i was reduced and was abolished after extracellular Ca2+ removal or nifedipine. Removal of extracellular Ca2+ reduced ET-1-induced tension; however, nifedipine had only a slight effect. These data indicate that maintenance of resting [Ca2+]i in PASMCs from chronically hypoxic rats does not require activation of L-type Ca2+ channels and suggest that ET-1-induced contraction occurs by a mechanism primarily independent of changes in [Ca2+]i.

intracellular calcium concentration; endothelin-1; chronic hypoxia; pulmonary hypertension; contraction; voltage-gated calcium channels; smooth muscle; pulmonary artery smooth muscle cells


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. R. S. Broughton, B. R. Walker, and T. C. Resta
Chronic hypoxia induces Rho kinase-dependent myogenic tone in small pulmonary arteries
Am J Physiol Lung Cell Mol Physiol, April 1, 2008; 294(4): L797 - L806.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. M. Whitman, S. Pisarcik, T. Luke, M. Fallon, J. Wang, J. T. Sylvester, G. L. Semenza, and L. A. Shimoda
Endothelin-1 mediates hypoxia-induced inhibition of voltage-gated K+ channel expression in pulmonary arterial myocytes
Am J Physiol Lung Cell Mol Physiol, February 1, 2008; 294(2): L309 - L318.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. A. Shimoda, T. Luke, J. T. Sylvester, H.-W. Shih, A. Jain, and E. R. Swenson
Inhibition of hypoxia-induced calcium responses in pulmonary arterial smooth muscle by acetazolamide is independent of carbonic anhydrase inhibition
Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L1002 - L1012.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Hinton, A. Gutsol, and S. Dakshinamurti
Thromboxane hypersensitivity in hypoxic pulmonary artery myocytes: altered TP receptor localization and kinetics
Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L654 - L663.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
P. I. Aaronson
TRPC Channel Upregulation in Chronically Hypoxic Pulmonary Arteries: The HIF-1 Bandwagon Gathers Steam
Circ. Res., June 23, 2006; 98(12): 1465 - 1467.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. L. Jernigan, B. R. S. Broughton, B. R. Walker, and T. C. Resta
Impaired NO-dependent inhibition of store- and receptor-operated calcium entry in pulmonary vascular smooth muscle after chronic hypoxia
Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L517 - L525.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. R. H. Mauban, K. Wilkinson, C. Schach, and J. X.-J. Yuan
Histamine-mediated increases in cytosolic [Ca2+] involve different mechanisms in human pulmonary artery smooth muscle and endothelial cells
Am J Physiol Cell Physiol, February 1, 2006; 290(2): C325 - C336.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Weigand, J. T. Sylvester, and L. A. Shimoda
Mechanisms of endothelin-1-induced contraction in pulmonary arteries from chronically hypoxic rats
Am J Physiol Lung Cell Mol Physiol, February 1, 2006; 290(2): L284 - L290.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Hinton, L. Mellow, A. J. Halayko, A. Gutsol, and S. Dakshinamurti
Hypoxia induces hypersensitivity and hyperreactivity to thromboxane receptor agonist in neonatal pulmonary arterial myocytes
Am J Physiol Lung Cell Mol Physiol, February 1, 2006; 290(2): L375 - L384.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. J. Rios, M. Fallon, J. Wang, and L. A. Shimoda
Chronic hypoxia elevates intracellular pH and activates Na+/H+ exchange in pulmonary arterial smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L867 - L874.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Wang, L. Weigand, W. Wang, J. T. Sylvester, and L. A. Shimoda
Chronic hypoxia inhibits Kv channel gene expression in rat distal pulmonary artery
Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1049 - L1058.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. Christou, N. Bailey, M. S. Kluger, S. A. Mitsialis, and S. Kourembanas
Extracellular acidosis induces heme oxygenase-1 expression in vascular smooth muscle cells
Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2647 - H2652.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. S. Naik, S. Earley, T. C. Resta, and B. R. Walker
Pressure-induced smooth muscle cell depolarization in pulmonary arteries from control and chronically hypoxic rats does not cause myogenic vasoconstriction
J Appl Physiol, March 1, 2005; 98(3): 1119 - 1124.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. L. Jernigan, B. R. Walker, and T. C. Resta
Chronic hypoxia augments protein kinase G-mediated Ca2+ desensitization in pulmonary vascular smooth muscle through inhibition of RhoA/Rho kinase signaling
Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1220 - L1229.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. L. Jernigan, B. R. Walker, and T. C. Resta
Endothelium-derived reactive oxygen species and endothelin-1 attenuate NO-dependent pulmonary vasodilation following chronic hypoxia
Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L801 - L808.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M.-J. Lin, G. P.H. Leung, W.-M. Zhang, X.-R. Yang, K.-P. Yip, C.-M. Tse, and J. S.K. Sham
Chronic Hypoxia-Induced Upregulation of Store-Operated and Receptor-Operated Ca2+ Channels in Pulmonary Arterial Smooth Muscle Cells: A Novel Mechanism of Hypoxic Pulmonary Hypertension
Circ. Res., September 3, 2004; 95(5): 496 - 505.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
O. Pauvert, S. Bonnet, E. Rousseau, R. Marthan, and J.-P. Savineau
Sildenafil alters calcium signaling and vascular tone in pulmonary arteries from chronically hypoxic rats
Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L577 - L583.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
V. Sauzeau, M. Rolli-Derkinderen, S. Lehoux, G. Loirand, and P. Pacaud
Sildenafil Prevents Change in RhoA Expression Induced by Chronic Hypoxia in Rat Pulmonary Artery
Circ. Res., October 3, 2003; 93(7): 630 - 637.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Yu, M. Sweeney, S. Zhang, O. Platoshyn, J. Landsberg, A. Rothman, and J. X.-J. Yuan
PDGF stimulates pulmonary vascular smooth muscle cell proliferation by upregulating TRPC6 expression
Am J Physiol Cell Physiol, February 1, 2003; 284(2): C316 - C330.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Bonnet, A. Belus, J.-M. Hyvelin, E. Roux, R. Marthan, and J.-P. Savineau
Effect of chronic hypoxia on agonist-induced tone and calcium signaling in rat pulmonary artery
Am J Physiol Lung Cell Mol Physiol, July 1, 2001; 281(1): L193 - L201.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. A. Shimoda, D. J. Manalo, J. S. K. Sham, G. L. Semenza, and J. T. Sylvester
Partial HIF-1{alpha} deficiency impairs pulmonary arterial myocyte electrophysiological responses to hypoxia
Am J Physiol Lung Cell Mol Physiol, July 1, 2001; 281(1): L202 - L208.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. A. Madden
Focus on "Hypoxic constriction of porcine distal pulmonary arteries: endothelium and endothelin dependence"
Am J Physiol Lung Cell Mol Physiol, May 1, 2001; 280(5): L853 - L855.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online