AJP - Lung Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 279: L958-L966, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhao, G.
Right arrow Articles by Kao, P. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhao, G.
Right arrow Articles by Kao, P. N.
Vol. 279, Issue 5, L958-L966, November 2000

Anti-inflammatory effects of triptolide in human bronchial epithelial cells

Guohua Zhao, Laszlo T. Vaszar, Daoming Qiu, Lingfang Shi, and Peter N. Kao

Pulmonary and Critical Care Medicine, Stanford University Medical Center, Stanford, California 94305-5236

Triptolide (PG490, 97% pure) is a diterpenoid triepoxide with potent anti-inflammatory and immunosuppressive effects in transformed human bronchial epithelial cells and T cells (Qiu D, Zhao G, Aoki Y, Shi L, Uyei A, Nazarian S, Ng JC-H, and Kao PN. J Biol Chem 274: 13443-13450, 1999). Triptolide, with an IC50 of ~20-50 ng/ml, inhibits normal and transformed human bronchial epithelial cell expression of interleukin (IL)-6 and IL-8 stimulated by phorbol 12-myristate 13-acetate (PMA), tumor necrosis factor-alpha , or IL-1beta . Nuclear runoff and luciferase reporter gene assays demonstrate that triptolide inhibits IL-8 transcription. Triptolide also inhibits the transcriptional activation, but not the DNA binding, of nuclear factor-kappa B. A cDNA array and clustering algorithm analysis reveals that triptolide inhibits expression of the PMA-induced genes tumor necrosis factor-alpha , IL-8, macrophage inflammatory protein-2alpha , intercellular adhesion molecule-1, integrin beta 6, vascular endothelial growth factor, granulocyte-macrophage colony-stimulating factor, GATA-3, fra-1, and NF45. Triptolide also inhibits constitutively expressed cell cycle regulators and survival genes cyclins D1, B1, and A1, cdc-25, bcl-x, and c-jun. Thus anti-inflammatory, antiproliferative, and proapoptotic properties of triptolide are associated with inhibition of nuclear factor-kappa B signaling and inhibition of genes known to regulate cell cycle progression and survival.

Tripterygium; interleukin-8; transcription; nuclear factor-kappa B; Atlas cDNA array; cyclin


This article has been cited by other articles:


Home page
 Cancer Res.Home page
S. J. Leuenroth and C. M. Crews
Triptolide-Induced Transcriptional Arrest Is Associated with Changes in Nuclear Substructure
Cancer Res., July 1, 2008; 68(13): 5257 - 5266.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Y. Lu, Y. Liu, K. Fukuda, Y. Nakamura, N. Kumagai, and T. Nishida
Inhibition by triptolide of chemokine, proinflammatory cytokine, and adhesion molecule expression induced by lipopolysaccharide in corneal fibroblasts.
Invest. Ophthalmol. Vis. Sci., September 1, 2006; 47(9): 3796 - 3800.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
X. Chen, T. Murakami, J. J. Oppenheim, and O. M. Z. Howard
Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking
Blood, October 1, 2005; 106(7): 2409 - 2416.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
L. T. Vaszar, T. Nishimura, J. D. Storey, G. Zhao, D. Qiu, J. L. Faul, R. G. Pearl, and P. N. Kao
Longitudinal transcriptional analysis of developing neointimal vascular occlusion and pulmonary hypertension in rats
Physiol Genomics, April 13, 2004; 17(2): 150 - 156.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Y. Ma, G. K. Iwamoto, N. T. Hoa, V. Akotia, A. Pedram, M. A. Boivin, and H. M. Said
TNF-{alpha}-induced increase in intestinal epithelial tight junction permeability requires NF-{kappa}B activation
Am J Physiol Gastrointest Liver Physiol, March 1, 2004; 286(3): G367 - G376.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
T. Nishimura, J. L. Faul, G. J. Berry, L. T. Vaszar, D. Qiu, R. G. Pearl, and P. N. Kao
Simvastatin Attenuates Smooth Muscle Neointimal Proliferation and Pulmonary Hypertension in Rats
Am. J. Respir. Crit. Care Med., November 15, 2002; 166(10): 1403 - 1408.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
T. M. Kiviharju, P. S. Lecane, R. G. Sellers, and D. M. Peehl
Antiproliferative and Proapoptotic Activities of Triptolide (PG490), a Natural Product Entering Clinical Trials, on Primary Cultures of Human Prostatic Epithelial Cells
Clin. Cancer Res., August 1, 2002; 8(8): 2666 - 2674.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online