| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Anesthesiology, 2 Biochemistry and Molecular Genetics, and 3 Pharmacology and 4 The Center for Free Radical Biology, The University of Alabama at Birmingham, Birmingham, Alabama 35233-4234
Ventilator strategies allowing for
increases in carbon dioxide (CO2) tensions (hypercapnia)
are being emphasized to ameliorate the consequences of
inflammatory-mediated lung injury. Inflammatory responses lead to the
generation of reactive species including superoxide
(O2
), nitric oxide (·NO), and their product
peroxynitrite (ONOO). The reaction of CO2 and
ONOO can yield the nitrosoperoxocarbonate adduct
ONOOCO2, a more potent nitrating species than
ONOO. Based on these premises, monolayers of fetal rat
alveolar epithelial cells were utilized to investigate whether
hypercapnia would modify pathways of ·NO production and reactivity
that impact pulmonary metabolism and function. Stimulated cells exposed
to 15% CO2 (hypercapnia) revealed a significant increase
in ·NO production and nitric oxide synthase (NOS) activity. Cell
3-nitrotyrosine content as measured by both HPLC and immunofluorescence
staining also increased when exposed to these same conditions.
Hypercapnia significantly enhanced cell injury as evidenced by
impairment of monolayer barrier function and increased induction of
apoptosis. These results were attenuated by the NOS inhibitor
N-monomethyl-L-arginine. Our studies reveal that
hypercapnia modifies ·NO-dependent pathways to amplify cell injury.
These results affirm the underlying role of ·NO in tissue inflammatory reactions and reveal the impact of hypercapnia on inflammatory reactions and its potential detrimental influences.
carbon dioxide; nitration; inflammation; superoxide; free radical; peroxynitrite
This article has been cited by other articles:
|
|
 |
|
  I. T. Helenius, T. Krupinski, D. W. Turnbull, Y. Gruenbaum, N. Silverman, E. A. Johnson, P. H. S. Sporn, J. I. Sznajder, and G. J. Beitel Elevated CO2 suppresses specific Drosophila innate immune responses and resistance to bacterial infection PNAS, November 3, 2009; 106(44): 18710 - 18715. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Ketabchi, B. Egemnazarov, R. T. Schermuly, H. A. Ghofrani, W. Seeger, F. Grimminger, M. Shid-Moosavi, G. A. Dehghani, N. Weissmann, and N. Sommer Effects of hypercapnia with and without acidosis on hypoxic pulmonary vasoconstriction Am J Physiol Lung Cell Mol Physiol, November 1, 2009; 297(5): L977 - L983. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D O'Toole, P Hassett, M Contreras, B D Higgins, S T W McKeown, D F McAuley, T O'Brien, and J G Laffey Hypercapnic acidosis attenuates pulmonary epithelial wound repair by an NF-{kappa}B dependent mechanism Thorax, November 1, 2009; 64(11): 976 - 982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Abolhassani, A. Guais, P. Chaumet-Riffaud, A. J. Sasco, and L. Schwartz Carbon dioxide inhalation causes pulmonary inflammation Am J Physiol Lung Cell Mol Physiol, April 1, 2009; 296(4): L657 - L665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Caples, D. L. Rasmussen, W. Y. Lee, M. Z. Wolfert, and R. D. Hubmayr Impact of buffering hypercapnic acidosis on cell wounding in ventilator-injured rat lungs Am J Physiol Lung Cell Mol Physiol, January 1, 2009; 296(1): L140 - L144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Wang, F. Su, A. Bruhn, X. Yang, and J.-L. Vincent Acute Hypercapnia Improves Indices of Tissue Oxygenation More than Dobutamine in Septic Shock Am. J. Respir. Crit. Care Med., January 15, 2008; 177(2): 178 - 183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kantores, P. J. McNamara, L. Teixeira, D. Engelberts, P. Murthy, B. P. Kavanagh, and R. P. Jankov Therapeutic hypercapnia prevents chronic hypoxia-induced pulmonary hypertension in the newborn rat Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L912 - L922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Lang, P. Dong, E. K. Hosszu, and I. R. Doyle Effect of CO2 on LPS-induced cytokine responses in rat alveolar macrophages Am J Physiol Lung Cell Mol Physiol, July 1, 2005; 289(1): L96 - L103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Janssen-Heininger, K. Ckless, N. Reynaert, and A. van der Vliet SOD Inactivation in Asthma: Bad News or NO News? Am. J. Pathol., March 1, 2005; 166(3): 649 - 652. [Full Text] [PDF]
|
|
|
|
|
|
B. P. Kavanagh Therapeutic Hypercapnia: Careful Science, Better Trials Am. J. Respir. Crit. Care Med., January 15, 2005; 171(2): 96 - 97. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Arbogast and M. B. Reid Oxidant activity in skeletal muscle fibers is influenced by temperature, CO2 level, and muscle-derived nitric oxide Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2004; 287(4): R698 - R705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Laffey, D. Honan, N. Hopkins, J.-M. Hyvelin, J. F. Boylan, and P. McLoughlin Hypercapnic Acidosis Attenuates Endotoxin-induced Acute Lung Injury Am. J. Respir. Crit. Care Med., January 1, 2004; 169(1): 46 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Gerlach, D. Keh, A. Semmerow, T. Busch, K. Lewandowski, D. M. Pappert, R. Rossaint, and K. J. Falke Dose-Response Characteristics during Long-Term Inhalation of Nitric Oxide in Patients with Severe Acute Respiratory Distress Syndrome: A Prospective, Randomized, Controlled Study Am. J. Respir. Crit. Care Med., April 1, 2003; 167(7): 1008 - 1015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Baldus, L. Castro, J. P. Eiserich, and B. A. Freeman Is {middle dot}NO News Bad News in Acute Respiratory Distress Syndrome? Am. J. Respir. Crit. Care Med., February 1, 2001; 163(2): 308 - 310. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
