AJP - Lung AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 279: L1103-L1109, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gladwin, M. T.
Right arrow Articles by Shelhamer, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gladwin, M. T.
Right arrow Articles by Shelhamer, J. H.
Vol. 279, Issue 6, L1103-L1109, December 2000

Retinoic acid reduces p11 protein levels in bronchial epithelial cells by a posttranslational mechanism

M. T. Gladwin, X. L. Yao, M. Cowan, X. L. Huang, R. Schneider, L. R. Grant, C. Logun, and J. H. Shelhamer

Critical Care Medicine Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892

p11 is a member of the S100 family of proteins, is the cellular ligand of annexin II, and interacts with the carboxyl region of 85-kDa cytosolic phospholipase A2 (cPLA2), inhibiting cPLA2 activity and arachidonic acid (AA) release. We studied the effect of retinoic acid (RA) on PLA2 activity in human bronchial epithelial cells and whether p11 contributes to these effects. The addition of 10-6 M RA resulted in reduced p11 protein levels at 4 days, with the greatest effect observed on days 6 and 7. This effect was dose related (10-6 to 10-9 M). RA treatment (10-6 M) had no effect on cPLA2 protein levels. p11 mRNA levels were unchanged at 6 and 8 days of treatment (correlating with maximum p11 protein reduction). Treatment with RA reduced p11 levels in control cells and in cells transfected with a p11 expression vector, suggesting a posttranslational mechanism. Lactacystin (10-6 M), an inhibitor of the human 26S proteasome, blocked the decrease in p11 observed with RA treatment. Compared with control cells (n = 3), RA-treated cells (n = 3) had significantly increased AA release after treatment with the calcium ionophore A-23187 (P = 0.006). Therefore, RA reduces p11 protein expression and increases PLA2 activity and AA release.

annexin II light chain; cytosolic phospholipase A2; arachidonic acid


This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
J. S. Moreb, A. Gabr, G. R. Vartikar, S. Gowda, J. R. Zucali, and D. Mohuczy
Retinoic Acid Down-Regulates Aldehyde Dehydrogenase and Increases Cytotoxicity of 4-Hydroperoxycyclophosphamide and Acetaldehyde
J. Pharmacol. Exp. Ther., January 1, 2005; 312(1): 339 - 345.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X.-l. Huang, R. Pawliczak, X.-l. Yao, M. J. Cowan, M. T. Gladwin, M. J. Walter, M. J. Holtzman, P. Madara, C. Logun, and J. H. Shelhamer
Interferon-gamma Induces p11 Gene and Protein Expression in Human Epithelial Cells through Interferon-gamma -activated Sequences in the p11 Promoter
J. Biol. Chem., March 7, 2003; 278(11): 9298 - 9308.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online