AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 279: L1110-L1119, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (41)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wodopia, R.
Right arrow Articles by Mairbäurl, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wodopia, R.
Right arrow Articles by Mairbäurl, H.
Vol. 279, Issue 6, L1110-L1119, December 2000

Hypoxia decreases proteins involved in epithelial electrolyte transport in A549 cells and rat lung

Ralf Wodopia1, Hyun Soo Ko1, Javiera Billian1, Rudolf Wiesner2, Peter Bärtsch1, and Heimo Mairbäurl1

1 Medical Clinic, Section Sports Medicine, University of Heidelberg, 69115 Heidelberg; and 2 Department of Vegetative Physiology, University of Cologne, 50931 Cologne, Germany

Fluid reabsorption from alveolar space is driven by active Na reabsorption via epithelial Na channels (ENaCs) and Na-K-ATPase. Both are inhibited by hypoxia. Here we tested whether hypoxia decreases Na transport by decreasing the number of copies of transporters in alveolar epithelial cells and in lungs of hypoxic rats. Membrane fractions were prepared from A549 cells exposed to hypoxia (3% O2) as well as from whole lung tissue and alveolar type II cells from rats exposed to hypoxia. Transport proteins were measured by Western blot analysis. In A549 cells, alpha 1- and beta 1-Na-K-ATPase, Na/K/2Cl cotransport, and ENaC proteins decreased during hypoxia. In whole lung tissue, alpha 1-Na-K-ATPase and Na/K/2Cl cotransport decreased. alpha - and beta -ENaC mRNAs also decreased in hypoxic lungs. Similar results were seen in alveolar type II cells from hypoxic rats. These results indicate a slow decrease in the amount of Na-transporting proteins in alveolar epithelial cells during exposure to hypoxia that also occurs in vivo in lungs from hypoxic animals. The reduced number of transporters might account for the decreased transport activity and impaired edema clearance in hypoxic lungs.

hypoxic pulmonary edema; sodium-potassium-adenosine 5'-triphosphatase; sodium/potassium/2 chloride cotransport; epithelial sodium channel; alveolar epithelium; expression


This article has been cited by other articles:


Home page
 Eur Respir JHome page
G. Zhou, L. A. Dada, and J. I. Sznajder
Regulation of alveolar epithelial function by hypoxia
Eur. Respir. J., May 1, 2008; 31(5): 1107 - 1113.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Guney, A. Schuler, A. Ott, S. Hoschele, S. Zugel, E. Baloglu, P. Bartsch, and H. Mairbaurl
Dexamethasone prevents transport inhibition by hypoxia in rat lung and alveolar epithelial cells by stimulating activity and expression of Na+-K+-ATPase and epithelial Na+ channels
Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1332 - L1338.
[Abstract] [Full Text] [PDF]

Home page
 Integr. Comp. Biol.Home page
D. Hoogewijs, N. B. Terwilliger, K. A. Webster, J. A. Powell-Coffman, S. Tokishita, H. Yamagata, T. Hankeln, T. Burmester, K. T. Rytkonen, M. Nikinmaa, et al.
From critters to cancers: bridging comparative and clinical research on oxygen sensing, HIF signaling, and adaptations towards hypoxia
Integr. Comp. Biol., October 1, 2007; 47(4): 552 - 577.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. Andrade, A. C. Rodrigues Jr., T. R. C. Sanches, R. B. Souza, and A. C. Seguro
Leptospirosis leads to dysregulation of sodium transporters in the kidney and lung
Am J Physiol Renal Physiol, February 1, 2007; 292(2): F586 - F592.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. Maggiorini
High altitude-induced pulmonary oedema
Cardiovasc Res, October 1, 2006; 72(1): 41 - 50.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. M. Mutlu and J. I. Sznajder
Mechanisms of pulmonary edema clearance
Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L685 - L695.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
M. Jain and J. I. Sznajder
Effects of Hypoxia on the Alveolar Epithelium
Proceedings of the ATS, October 1, 2005; 2(3): 202 - 205.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. Karle, T. Gehrig, R. Wodopia, S. Hoschele, V. A. W. Kreye, H. A. Katus, P. Bartsch, and H. Mairbaurl
Hypoxia-induced inhibition of whole cell membrane currents and ion transport of A549 cells
Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1154 - L1160.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. M. Hardiman, C. M. McNicholas-Bevensee, J. Fortenberry, C. T. Myles, B. Malik, D. C. Eaton, and S. Matalon
Regulation of Amiloride-Sensitive Na+ Transport by Basal Nitric Oxide
Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 720 - 728.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Dagenais, R. Frechette, Y. Yamagata, T. Yamagata, J.-F. Carmel, M.-E. Clermont, E. Brochiero, C. Masse, and Y. Berthiaume
Downregulation of ENaC activity and expression by TNF-{alpha} in alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L301 - L311.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
H. Mairbaurl, F. Schwobel, S. Hoschele, M. Maggiorini, S. Gibbs, E. R. Swenson, and P. Bartsch
Altered ion transporter expression in bronchial epithelium in mountaineers with high-altitude pulmonary edema
J Appl Physiol, November 1, 2003; 95(5): 1843 - 1850.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. P. Comellas, L. M. Pesce, Z. Azzam, F. J. Saldias, and J. I. Sznajder
Scorpion Venom Decreases Lung Liquid Clearance in Rats
Am. J. Respir. Crit. Care Med., April 15, 2003; 167(8): 1064 - 1067.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
S. Hoschele and H. Mairbaurl
Alveolar Flooding at High Altitude: Failure of Reabsorption?
Physiology, April 1, 2003; 18(2): 55 - 59.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Jovanovic, R. M. Crawford, H. J. Ranki, and A. Jovanovic
Large Conductance Ca2+-Activated K+ Channels Sense Acute Changes in Oxygen Tension in Alveolar Epithelial Cells
Am. J. Respir. Cell Mol. Biol., March 1, 2003; 28(3): 363 - 372.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
U. H. Thome, I. C. Davis, S. V. Nguyen, B. J. Shelton, and S. Matalon
Modulation of sodium transport in fetal alveolar epithelial cells by oxygen and corticosterone
Am J Physiol Lung Cell Mol Physiol, February 1, 2003; 284(2): L376 - L385.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. C. Carpenter, S. Schomberg, C. Nichols, K. R. Stenmark, and J. V. Weil
Hypoxia reversibly inhibits epithelial sodium transport but does not inhibit lung ENaC or Na-K-ATPase expression
Am J Physiol Lung Cell Mol Physiol, January 1, 2003; 284(1): L77 - L83.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. A. Matthay, H. G. Folkesson, and C. Clerici
Lung Epithelial Fluid Transport and the Resolution of Pulmonary Edema
Physiol Rev, July 1, 2002; 82(3): 569 - 600.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. M. Hardiman and S. Matalon
Modification of Sodium Transport and Alveolar Fluid Clearance by Hypoxia . Mechanisms and Physiological Implications
Am. J. Respir. Cell Mol. Biol., November 1, 2001; 25(5): 538 - 541.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Mairbaurl, K. Mayer, K.-J. Kim, Z. Borok, P. Bartsch, and E. D. Crandall
Alveolar Epithelial Ion and Fluid Transport: Hypoxia decreases active Na transport across primary rat alveolar epithelial cell monolayers
Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L659 - L665.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online