AJP - Lung AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 279: L1129-L1136, 2000;
1040-0605/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Browning, D. D.
Right arrow Articles by Ye, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Browning, D. D.
Right arrow Articles by Ye, R. D.
Vol. 279, Issue 6, L1129-L1136, December 2000

Autocrine regulation of interleukin-8 production in human monocytes

Darren D. Browning1,2, Wade C. Diehl2, Matthew H. Hsu2, Ingrid U. Schraufstatter2, and Richard D. Ye1,2

1 Department of Pharmacology, University of Illinois College of Medicine, Chicago, Illinois 60612; and 2 Department of Immunology, The Scripps Research Institute, La Jolla, California 92037

Interleukin (IL)-8 is a C-X-C chemokine that plays an important role in acute inflammation through its G protein-coupled receptors CXCR1 and CXCR2. In this study, we investigated the role of IL-8 as an autocrine regulator of IL-8 production and the signaling mechanisms involved in human peripheral blood mononuclear cells (MNCs). Sepharose-immobilized IL-8 stimulated a sevenfold increase in IL-8 production within 2 h. IL-8 induced the expression of its own message, and IL-8 biosynthesis was inhibited by cycloheximide and actinomycin D, indicating de novo RNA and protein synthesis. In contrast to MNCs, polymorphonuclear neutrophils did not respond to the immobilized IL-8 with IL-8 production despite cell surface expression of CXCR1 and CXCR2. Melanoma growth-stimulatory activity/growth-related protein-alpha (MGSA/GROalpha ), which binds CXCR2 but not CXCR1, was unable to either stimulate IL-8 secretion in MNCs or desensitize these cells to respond to immobilized IL-8. The involvement of mitogen-activated protein kinase (MAPK) in IL-8-induced IL-8 biosynthesis was suggested by the ability of PD-98059, an inhibitor of MAPK kinase, to block this function. Furthermore, IL-8 induced a significant increase in extracellular signal-regulated kinase 2 phosphorylation, whereas MGSA/GROalpha was much less effective. These findings support the role of IL-8 as an autocrine regulator of IL-8 production and suggest that this function is mediated by CXCR1 through activation of MAPK.

cytokines; chemokines; inflammation; monocytes


This article has been cited by other articles:


Home page
 Exp. Biol. Med.Home page
L. Stefanovic, D. A. Brenner, and B. Stefanovic
Direct Hepatotoxic Effect of KC Chemokine in the Liver Without Infiltration of Neutrophils
Experimental Biology and Medicine, September 1, 2005; 230(8): 573 - 586.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
W. C. Tsai, M. L. Rodriguez, K. S. Young, J. C. Deng, V. J. Thannickal, K. Tateda, M. B. Hershenson, and T. J. Standiford
Azithromycin Blocks Neutrophil Recruitment in Pseudomonas Endobronchial Infection
Am. J. Respir. Crit. Care Med., December 15, 2004; 170(12): 1331 - 1339.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Schraufstatter, H. Takamori, L. Sikora, P. Sriramarao, and R. G. DiScipio
Eosinophils and monocytes produce pulmonary and activation-regulated chemokine, which activates cultured monocytes/macrophages
Am J Physiol Lung Cell Mol Physiol, March 1, 2004; 286(3): L494 - L501.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. L. Podolin, B. J. Bolognese, J. J. Foley, D. B. Schmidt, P. T. Buckley, K. L. Widdowson, Q. Jin, J. R. White, J. M. Lee, R. B. Goodman, et al.
A Potent and Selective Nonpeptide Antagonist of CXCR2 Inhibits Acute and Chronic Models of Arthritis in the Rabbit
J. Immunol., December 1, 2002; 169(11): 6435 - 6444.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
R. D. Ye
Regulation of nuclear factor {kappa}B activation by G-protein-coupled receptors
J. Leukoc. Biol., December 1, 2001; 70(6): 839 - 848.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. W. Shepard, M. Yang, P. Xie, D. D. Browning, T. Voyno-Yasenetskaya, T. Kozasa, and R. D. Ye
Constitutive Activation of NF-kappa B and Secretion of Interleukin-8 Induced by the G Protein-coupled Receptor of Kaposi's Sarcoma-associated Herpesvirus Involve Galpha 13 and RhoA
J. Biol. Chem., November 30, 2001; 276(49): 45979 - 45987.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. B. Kuhns, E. L. Nelson, W. G. Alvord, and J. I. Gallin
Fibrinogen Induces IL-8 Synthesis in Human Neutrophils Stimulated with Formyl-Methionyl-Leucyl-Phenylalanine or Leukotriene B4
J. Immunol., September 1, 2001; 167(5): 2869 - 2878.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. R. Fischer, Y. Luo, M. Luo, L. Santambrogio, and M. E. Dorf
RANTES-Induced Chemokine Cascade in Dendritic Cells
J. Immunol., August 1, 2001; 167(3): 1637 - 1643.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online