AJP - Lung Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 280: L214-L220, 2001;
1040-0605/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Paananen, R.
Right arrow Articles by Hallman, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Paananen, R.
Right arrow Articles by Hallman, M.
Vol. 280, Issue 2, L214-L220, February 2001

Expression and localization of lung surfactant protein B in Eustachian tube epithelium

Reija Paananen1,2, Virpi Glumoff1,2, Raija Sormunen1,3, Wim Voorhout4, and Mikko Hallman1,2

1 Biocenter Oulu, 2 Department of Pediatrics, and 3 Department of Pathology, University of Oulu, 90014 Oulu, Finland; and 4 Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, 3508 TD Utrecht, The Netherlands

Surfactant protein (SP) B is an essential component of the pulmonary surfactant complex, which participates in reducing the surface tension across the alveolar air-liquid interface. The Eustachian tube (ET) connects the upper respiratory tract to the middle ear, serving as an intermittent airway between the pharynx and the middle ear. Recently, we described the expression of SP-A and SP-D in the ET, suggesting their role in middle ear host defense. Our present aim was to detect whether the expression of SP-B is evident in the porcine ET. With Northern blot analysis, RT-PCR, and in situ hybridizations, SP-B mRNA was identified and localized in the ET epithelium. The cellular localization of SP-B was revealed with immunohistochemistry, electron microscopy, and immunoelectron microscopy. The protein was found in the secretory granules of epithelial cells and also attached to the microvilli at the luminal side of these cells. The SP-B immunoreactivity of aggregates isolated from ET lavage fluid was similar to that isolated from bronchoalveolar lavage fluid. We conclude that there are specialized cells in the ET epithelium expressing and secreting SP-B and propose that SP-B may facilitate normal opening of the tube and mucociliary transport.

in situ hybridization; immunoelectron microscopy; messenger ribonucleic acid


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. K. Kim, S.-S. Kim, K. W. Rha, C.-H. Kim, J. H. Cho, C.-H. Lee, J.-G. Lee, and J.-H. Yoon
Expression and localization of surfactant proteins in human nasal epithelium
Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L879 - L884.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Strayer, R. C. Savani, L. W. Gonzales, A. Zaman, Z. Cui, E. Veszelovszky, E. Wood, Y.-S. Ho, and P. L. Ballard
Pre- and Postnatal Lung Development, Maturation, and Plasticity: Human surfactant protein B promoter in transgenic mice: temporal, spatial, and stimulus-responsive regulation
Am J Physiol Lung Cell Mol Physiol, March 1, 2002; 282(3): L394 - L404.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
R. Paananen, A. D. Postle, G. Clark, V. Glumoff, and M. Hallman
Eustachian tube surfactant is different from alveolar surfactant: determination of phospholipid composition of porcine eustachian tube lavage fluid
J. Lipid Res., January 1, 2002; 43(1): 99 - 106.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. Paananen, R. Sormunen, V. Glumoff, M. van Eijk, and M. Hallman
Surfactant proteins A and D in Eustachian tube epithelium
Am J Physiol Lung Cell Mol Physiol, September 1, 2001; 281(3): L660 - L667.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online