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Departments of 1 Neurology and 3 Medicine, The Medical College of Wisconsin, Milwaukee 53226; and 2 Research Service, Zablocki Veterans Affairs Medical Center, Milwaukee, Wisconsin 53295
The purposes of this study were to
determine 1) the presence of the major ion transport
activities that regulate cytoplasmic pH (pHc) in cat
pulmonary artery smooth muscle cells, i.e.,
Na+/H+ and the Na+-dependent and
-independent Cl
/HCO3 exchange,
2) whether pHc changes in cells from small
(SPAs) and large (LPAs) pulmonary arteries during hypoxia, and
3) whether changes in pHc are due to changes in
the balance of exchange activities. Exchange activities as defined by
physiological maneuvers rather than molecular identity were ascertained
with fluorescence microscopy to document changes in the ratio of the
pHc indicator
2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein. Steady-state
pHc was higher in LPA than in SPA normoxic smooth muscle
cells. SPAs and LPAs possessed all three transport activities; in
HCO3-containing normoxic solutions,
Cl/HCO3 exchange rather than
Na+/H+ exchange set the level of
pHc; in HCO3-containing hypoxic
solutions, pHc increased in SPA and decreased in LPA cells;
altering the baseline pHc of a cell type to that of the
other did not change the direction of the pHc response during hypoxia. The absence of Na+ prevented
hypoxia-induced alkalinization in SPA cells; in both cell types,
inhibiting the Cl/HCO3 exchange
activities reversed the normal direction of pHc changes during hypoxia.
cat; sodium/hydrogen exchange; sodium-dependent chloride/bicarbonate exchange; sodium-independent chloride/bicarbonate exchange
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