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1 Center For Environmental Medicine and Lung Biology, University of North Carolina, Chapel Hill 27599; and 2 National Health and Environmental Effects Research Laboratory, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711
Little is known
about the functional capabilities of bronchial macrophages (BMs) and
their relationship to airway disease such as asthma. We hypothesize
that BMs from asthmatics may be modulated in their function compared
with similar cells from healthy individuals. BMs obtained by induced
sputum from mild asthmatics (n = 20) and healthy
individuals (n = 20) were analyzed using flow cytometry for
CD16, CD64, CD11b, CD14, and human leukocyte antigen-DR expression,
phagocytosis of IgG opsonized yeast, and oxidant production. Asthma
status was assessed by lung function [percent predicted forced vital
capacity and forced expiratory volume in 1 s
(FEV1)], percent sputum eosinophils, and nonspecific airway responsiveness [provocative concentration that produces a
20% fall in FEV1 (PC20,FEV1)]. Asthmatics
with >5% airway eosinophils (AEo+) had decreased BM CD64 expression
and phagocytosis compared with asthmatics with <5% eosinophils
(AEo
). Among asthmatics, a significant correlation was found between
CD64 expression and BM phagocytosis (R = 0.7, P < 0.009). Phagocytosis was also correlated with
PC20,FEV1 (R = 0.6, P < 0.007), lung function (%predicted FEV1, R = 0.7, P < 0.002) and percent eosinophils (R
= 0.6, P < 0.01). In conclusion, BM from
asthmatics are functionally modulated, possibly by Th2 cytokines
involved in asthma pathology.
asthma; bronchial macrophages; induced sputum; flow cytometry analysis of surface receptors; CD64; CD11b; phagocytosis
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