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Am J Physiol Lung Cell Mol Physiol 280: L1057-L1065, 2001;
1040-0605/01 $5.00
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Vol. 280, Issue 5, L1057-L1065, May 2001

SPECIAL COMMUNICATION
Isolation of pulmonary interstitial fluid in rabbits by a modified wick technique

Daniela Negrini1, Alberto Passi2, Katia Bertin1, Federica Bosi3, and Helge Wiig4

1 Department of Medicine, Surgery and Dentistry, University of Milano, 20133 Milan; 2 Department of Experimental and Clinical Biomedical Science and 3 Department of Clinical and Biological Sciences, University of Insubria, 21100 Varese, Italy; and 4 Department of Physiology, University of Bergen, N-5000 Bergen, Norway

Interstitial fluid protein concentration (Cprotein) values in perivascular and peribronchial lung tissues were never simultaneously measured in mammals; in this study, perivascular and peribronchial interstitial fluids were collected from rabbits under control conditions and rabbits with hydraulic edema or lesional edema. Postmortem dry wicks were implanted in the perivascular and peribronchial tissues; after 20 min, the wicks were withdrawn and the interstitial fluid was collected to measure Cprotein and colloid osmotic pressure. Plasma, perivascular, and peribronchial Cprotein values averaged 6.4 ± 0.7 (SD), 3.7 ± 0.5, and 2.4 ± 0.7 g/dl, respectively, in control rabbits; 4.8 ± 0.7, 2.5 ± 0.6, and 2.4 ± 0.4 g/dl, respectively, in rabbits with hydraulic edema; and 5.1 ± 0.3, 4.3 ± 0.4 and 3.3 ± 0.6 g/dl, respectively, in rabbits with lesional edema. Contamination of plasma proteins from microvascular lesions during wick insertion was 14% of plasma Cprotein. In control animals, pulmonary interstitial Cprotein was lower than previous estimates from pre- and postnodal pulmonary lymph; furthermore, although the interstitium constitutes a continuum within the lung parenchyma, regional differences in tissue content seem to exist in the rabbit lung.

lung edema; lung fluid balance; regional pulmonary interstitial protein concentration


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