AJP - Lung Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 280: L965-L973, 2001;
1040-0605/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (27)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Benoit, C.
Right arrow Articles by Rousseau, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Benoit, C.
Right arrow Articles by Rousseau, E.
Vol. 280, Issue 5, L965-L973, May 2001

EETs relax airway smooth muscle via an EpDHF effect: BKCa channel activation and hyperpolarization

Catherine Benoit, Barbara Renaudondagger, Dany Salvail, and Eric Rousseau

Le Bilarium, Faculty of Medicine, Department of Physiology and Biophysics, University of Sherbrooke, Sherbrooke, Quebec, Canada J1H 5N4

Epoxyeicosatrienoic acids (EETs) are produced from arachidonic acid via the cytochrome P-450 epoxygenase pathway. EETs are able to modulate smooth muscle tone by increasing K+ conductance, hence generating hyperpolarization of the tissues. However, the molecular mechanisms by which EETs induce smooth muscle relaxation are not fully understood. In the present study, the effects of EETs on airway smooth muscle (ASM) were investigated using three electrophysiological techniques. 8,9-EET and 14,15-EET induced concentration-dependent relaxations of the ASM precontracted with a muscarinc agonist (carbamylcholine chloride), and these relaxations were partly inhibited by 10 nM iberiotoxin (IbTX), a specific large-conductance Ca2+-activated K+ (BKCa) channel blocker. Moreover, 3 µM 8,9- or 14,15-EET induced hyperpolarizations of -12 ± 3.5 and -16 ± 3 mV, with EC50 values of 0.13 and 0.14 µM, respectively, which were either reversed or blocked on addition of 10 nM IbTX. These results indicate that BKCa channels are involved in hyperpolarization and participate in the relaxation of ASM. In addition, complementary experiments demonstrated that 8,9- and 14,15-EET activate reconstituted BKCa channels at low free Ca2+ concentrations without affecting their unitary conductance. These increases in channel activity were IbTX sensitive and correlated well with the IbTX-sensitive hyperpolarization and relaxation of ASM. Together these results support the view that, in ASM, the EETs act through an epithelium-derived hyperpolarizing factorlike effect.

epoxyeicosatrienoic acids; bronchorelaxation; membrane potential; potassium conductance; eicosanoids; planar lipid bilayers; epithelium-derived hyperpolarizing factors; large-conductance calcium-activated potassium channel

dagger Deceased 30 October 2000.




This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
P. Sun, W. Liu, D.-H. Lin, P. Yue, R. Kemp, L. M. Satlin, and W.-H. Wang
Epoxyeicosatrienoic Acid Activates BK Channels in the Cortical Collecting Duct
J. Am. Soc. Nephrol., March 1, 2009; 20(3): 513 - 523.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
E. K. Hoffmann, I. H. Lambert, and S. F. Pedersen
Physiology of Cell Volume Regulation in Vertebrates
Physiol Rev, January 1, 2009; 89(1): 193 - 277.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. Morin, M. Sirois, V. Echave, E. Rizcallah, and E. Rousseau
Relaxing effects of 17(18)-EpETE on arterial and airway smooth muscles in human lung
Am J Physiol Lung Cell Mol Physiol, January 1, 2009; 296(1): L130 - L139.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. Morin, C. Guibert, M. Sirois, V. Echave, M. M. Gomes, and E. Rousseau
Effects of {omega}-hydroxylase product on distal human pulmonary arteries
Am J Physiol Heart Circ Physiol, March 1, 2008; 294(3): H1435 - H1443.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. Morin, M. Sirois, V. Echave, M. M. Gomes, and E. Rousseau
EET Displays Anti-Inflammatory Effects in TNF-{alpha} Stimulated Human Bronchi: Putative Role of CPI-17
Am. J. Respir. Cell Mol. Biol., February 1, 2008; 38(2): 192 - 201.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. Morin, M. Sirois, V. Echave, M. M. Gomes, and E. Rousseau
Functional effects of 20-HETE on human bronchi: hyperpolarization and relaxation due to BKCa channel activation
Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L1037 - L1044.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. Morin, M. Sirois, V. Echave, M. M. Gomes, and E. Rousseau
Epoxyeicosatrienoic Acid Relaxing Effects Involve Ca2+-Activated K+ Channel Activation and CPI-17 Dephosphorylation in Human Bronchi
Am. J. Respir. Cell Mol. Biol., May 1, 2007; 36(5): 633 - 641.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. A. Spector and A. W. Norris
Action of epoxyeicosatrienoic acids on cellular function
Am J Physiol Cell Physiol, March 1, 2007; 292(3): C996 - C1012.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
Y.-Y. Dong, M. Wu, A. P.C. Yim, and G.-W. He
Hypoxia-Reoxygenation, St. Thomas Cardioplegic Solution, and Nicorandil on Endothelium-derived Hyperpolarizing Factor in Coronary Microarteries
Ann. Thorac. Surg., November 1, 2005; 80(5): 1803 - 1811.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. Hichami, C. Morin, E. Rousseau, and N. A. Khan
Diacylglycerol-Containing Docosahexaenoic Acid in Acyl Chain Modulates Airway Smooth Muscle Tone
Am. J. Respir. Cell Mol. Biol., October 1, 2005; 33(4): 378 - 386.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E Rousseau, M. Cloutier, C. Morin, and S. Proteau
Capsazepine, a vanilloid antagonist, abolishes tonic responses induced by 20-HETE on guinea pig airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L460 - L470.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
F. Mercier, C. Morin, M. Cloutier, S. Proteau, J. Rokach, W. S. Powell, and E. Rousseau
5-Oxo-ETE regulates tone of guinea pig airway smooth muscle via activation of Ca2+ pools and Rho-kinase pathway
Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L631 - L640.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Cloutier, S. Campbell, N. Basora, S. Proteau, M. D. Payet, and E. Rousseau
20-HETE inotropic effects involve the activation of a nonselective cationic current in airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L560 - L568.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. L. Clarke, S. Petrou, J. V. Walsh Jr., and J. J. Singer
Modulation of BKCa channel activity by fatty acids: structural requirements and mechanism of action
Am J Physiol Cell Physiol, November 1, 2002; 283(5): C1441 - C1453.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Salvail, M. Cloutier, and E. Rousseau
Functional reconstitution of an eicosanoid-modulated Cl- channel from bovine tracheal smooth muscle
Am J Physiol Cell Physiol, March 1, 2002; 282(3): C567 - C577.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online