AJP - Lung Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 280: L1094-L1103, 2001;
1040-0605/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schraufstatter, I. U.
Right arrow Articles by Burger, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schraufstatter, I. U.
Right arrow Articles by Burger, M.
Vol. 280, Issue 6, L1094-L1103, June 2001

IL-8 activates endothelial cell CXCR1 and CXCR2 through Rho and Rac signaling pathways

Ingrid U. Schraufstatter, Janice Chung, and Meike Burger

La Jolla Institute for Molecular Medicine, San Diego, California 92121

Stimulation of microvascular endothelial cells with interleukin (IL)-8 leads to cytoskeletal reorganization, which is mediated by combined activation of the CXCR1 and the CXCR2. In the early phase actin stress fibers appear, followed by cortical actin accumulation and cell retraction leading to gap formation between cells. The early response (between 1 and 5 min) is inhibited by an antibody that blocks the CXCR1. The later phase (from about 5 to 60 min), which is associated with cell retraction, is prevented by anti-CXCR2 antibody. Furthermore, anti-CXCR2, but not anti-CXCR1, antibody blocked IL-8-mediated haptotaxis of endothelial cells on collagen. The later phase of the IL-8-mediated actin response is inhibited by pertussis toxin, indicating that the CXCR2 couples to Gi. In contrast, the early phase is blocked by C3 botulinum toxin, which inactivates Rho, and by Y-27632, which inhibits Rho kinase, but not by pertussis toxin. Furthermore, the early CXCR1-mediated formation of stress fibers was prevented by dominant negative Rho. Dominant negative Rac on the other hand initially translocated to actin-rich filopodia after stimulation with IL-8 and later prevented cell retraction by blocking the CXCR2-mediated cytoskeletal response. These results indicate that IL-8 activates both the CXCR1 and the CXCR2 on microvascular endothelial cells, using different signal transduction cascades. The retraction of endothelial cells due to activation of the CXCR2 may contribute to the increased vascular permeability observed in acute inflammation and during the angiogenic response.

C-X-C chemokines; endothelial cells; interleukin-8 receptors; inflammation


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. C. Russo, R. Guabiraba, C. C. Garcia, L. S. Barcelos, E. Roffe, A. L. S. Souza, F. A. Amaral, D. Cisalpino, G. D. Cassali, A. Doni, et al.
Role of the Chemokine Receptor CXCR2 in Bleomycin-Induced Pulmonary Inflammation and Fibrosis
Am. J. Respir. Cell Mol. Biol., April 1, 2009; 40(4): 410 - 421.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
D. J.J. Waugh and C. Wilson
The Interleukin-8 Pathway in Cancer
Clin. Cancer Res., November 1, 2008; 14(21): 6735 - 6741.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
N. de la Iglesia, G. Konopka, K.-L. Lim, C. L. Nutt, J. F. Bromberg, D. A. Frank, P. S. Mischel, D. N. Louis, and A. Bonni
Deregulation of a STAT3-Interleukin 8 Signaling Pathway Promotes Human Glioblastoma Cell Proliferation and Invasiveness
J. Neurosci., June 4, 2008; 28(23): 5870 - 5878.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
L. Zheng, C.-n. Njauw, and M. Martins-Green
A hCXCR1 transgenic mouse model containing a conditional color-switching system for imaging of hCXCL8/IL-8 functions in vivo
J. Leukoc. Biol., November 1, 2007; 82(5): 1247 - 1256.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
D. J. Grab, E. Nyarko, N. C. Barat, O. V. Nikolskaia, and J. S. Dumler
Anaplasma phagocytophilum-Borrelia burgdorferi Coinfection Enhances Chemokine, Cytokine, and Matrix Metalloprotease Expression by Human Brain Microvascular Endothelial Cells
Clin. Vaccine Immunol., November 1, 2007; 14(11): 1420 - 1424.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. Sanchez, A. Moldobaeva, J. McClintock, J. Jenkins, and E. Wagner
The role of CXCR2 in systemic neovascularization of the mouse lung
J Appl Physiol, August 1, 2007; 103(2): 594 - 599.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
N. Inoue, T. Kondo, K. Kobayashi, M. Aoki, Y. Numaguchi, M. Shibuya, and T. Murohara
Therapeutic Angiogenesis Using Novel Vascular Endothelial Growth Factor-E/Human Placental Growth Factor Chimera Genes
Arterioscler. Thromb. Vasc. Biol., January 1, 2007; 27(1): 99 - 105.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. Yao, R.-H. Zhou, M. Petreaca, L. Zheng, J. Shyy, and M. Martins-Green
Activation of sterol regulatory element-binding proteins (SREBPs) is critical in IL-8-induced angiogenesis
J. Leukoc. Biol., September 1, 2006; 80(3): 608 - 620.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
H. C. Lane, A. R. Anand, and R. K. Ganju
Cbl and Akt regulate CXCL8-induced and CXCR1- and CXCR2-mediated chemotaxis
Int. Immunol., August 1, 2006; 18(8): 1315 - 1325.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
H. Miyazaki, V. Patel, H. Wang, R. K. Edmunds, J. S. Gutkind, and W. A. Yeudall
Down-regulation of CXCL5 Inhibits Squamous Carcinogenesis.
Cancer Res., April 15, 2006; 66(8): 4279 - 4284.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
D. M. Elaraj, D. M. Weinreich, S. Varghese, M. Puhlmann, S. M. Hewitt, N. M. Carroll, E. D. Feldman, E. M. Turner, and H. R. Alexander
The Role of Interleukin 1 in Growth and Metastasis of Human Cancer Xenografts
Clin. Cancer Res., February 15, 2006; 12(4): 1088 - 1096.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. Charalambous, L. B. Pen, Y. S. Su, J. Milan, T. C. Chen, and F. M. Hofman
Interleukin-8 Differentially Regulates Migration of Tumor-Associated and Normal Human Brain Endothelial Cells
Cancer Res., November 15, 2005; 65(22): 10347 - 10354.
[Abstract] [Full Text] [PDF]

Home page
 Neuro OncolHome page
D. J. Brat, A. C. Bellail, and E. G. Van Meir
The role of interleukin-8 and its receptors in gliomagenesis and tumoral angiogenesis
Neuro-oncol, April 1, 2005; 7(2): 122 - 133.
[Abstract] [PDF]

Home page
 BloodHome page
S. Emadi, D. Clay, C. Desterke, B. Guerton, E. Maquarre, A. Charpentier, C. Jasmin, M.-C. Le Bousse-Kerdiles, and for the French INSERM Research Network on MMM
IL-8 and its CXCR1 and CXCR2 receptors participate in the control of megakaryocytic proliferation, differentiation, and ploidy in myeloid metaplasia with myelofibrosis
Blood, January 15, 2005; 105(2): 464 - 473.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Zhao, A. Wimmer, K. Trieu, R. G. DiScipio, and I. U. Schraufstatter
Arrestin Regulates MAPK Activation and Prevents NADPH Oxidase-dependent Death of Cells Expressing CXCR2
J. Biol. Chem., November 19, 2004; 279(47): 49259 - 49267.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
D. Talavera, A. M. Castillo, M. C. Dominguez, A. E. Gutierrez, and I. Meza
IL8 release, tight junction and cytoskeleton dynamic reorganization conducive to permeability increase are induced by dengue virus infection of microvascular endothelial monolayers
J. Gen. Virol., July 1, 2004; 85(7): 1801 - 1813.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. N. Vanderbilt, E. M. Mager, L. Allen, T. Sawa, J. Wiener-Kronish, R. Gonzalez, and L. G. Dobbs
CXC Chemokines and Their Receptors Are Expressed in Type II Cells and Upregulated following Lung Injury
Am. J. Respir. Cell Mol. Biol., December 1, 2003; 29(6): 661 - 668.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. M. Stamatovic, R. F. Keep, S. L. Kunkel, and A. V. Andjelkovic
Potential role of MCP-1 in endothelial cell tight junction `opening': signaling via Rho and Rho kinase
J. Cell Sci., November 15, 2003; 116(22): 4615 - 4628.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Li, S. Dubey, M. L. Varney, B. J. Dave, and R. K. Singh
IL-8 Directly Enhanced Endothelial Cell Survival, Proliferation, and Matrix Metalloproteinases Production and Regulated Angiogenesis
J. Immunol., March 15, 2003; 170(6): 3369 - 3376.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
B. Heit, S. Tavener, E. Raharjo, and P. Kubes
An intracellular signaling hierarchy determines direction of migration in opposing chemotactic gradients
J. Cell Biol., October 14, 2002; 159(1): 91 - 102.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
I. U. Schraufstatter, K. Trieu, L. Sikora, P. Sriramarao, and R. DiScipio
Complement C3a and C5a Induce Different Signal Transduction Cascades in Endothelial Cells
J. Immunol., August 15, 2002; 169(4): 2102 - 2110.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. B. Huber, H. C. Reinhardt, M. Exner, J. A. Burger, D. Kerjaschki, M. A. Saleem, and H. Pavenstadt
Expression of Functional CCR and CXCR Chemokine Receptors in Podocytes
J. Immunol., June 15, 2002; 168(12): 6244 - 6252.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. A. Dzenko, A. V. Andjelkovic, W. A. Kuziel, and J. S. Pachter
The Chemokine Receptor CCR2 Mediates the Binding and Internalization of Monocyte Chemoattractant Protein-1 along Brain Microvessels
J. Neurosci., December 1, 2001; 21(23): 9214 - 9223.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online