AJP - Lung AJP: Renal Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 280: L1128-L1137, 2001;
1040-0605/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (23)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maeyama, T.
Right arrow Articles by Hara, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maeyama, T.
Right arrow Articles by Hara, N.
Vol. 280, Issue 6, L1128-L1137, June 2001

Attenuation of bleomycin-induced pneumopathy in mice by monoclonal antibody to interleukin-12

Takashige Maeyama, Kazuyoshi Kuwano, Masayuki Kawasaki, Ritsuko Kunitake, Naoki Hagimoto, and Nobuyuki Hara

Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Higashiku, Fukuoka 812-8582, Japan

We previously demonstrated essential roles of the Fas-Fas ligand (FasL) pathway in bleomycin-induced pneumopathy in mice. T lymphocytes and natural killer cells express FasL on activation and use it as a cytotoxic effector molecule. Because interleukin (IL)-12 is known to play a critical role in cell-mediated immunity, we investigated whether anti-IL-12 antibody treatment suppresses the development of this model. The anti-IL-12 antibody treatment decreased the number of apoptotic cells and the degree of inflammation and fibrosis in lung tissue. The results of RT-PCR showed that IL-12p40, IL-12 receptor (R) beta 2, interferon-gamma , tumor necrosis factor-alpha and FasL mRNAs were upregulated after bleomycin instillation. The upregulation of FasL, IL-12Rbeta 2, and tumor necrosis factor-alpha mRNA expression in lung tissue was suppressed by anti-IL-12 antibody treatment. The results of enzyme-linked immunosorbent assay showed that the levels of IL-12p40, but not of IL-12p70, were increased in lung tissue after bleomycin instillation. Although the increase in IL-12Rbeta 2 mRNA levels suggests that the T helper type 1 cell response may participate in lung injury, the increase in IL-12p40 supports T helper type 2 cell predominance in the fibrotic process of this model. The administration of anti-IL-12 antibody could be a novel therapy against lung injury and pulmonary fibrosis.

T helper type 1 cell; T helper type 2 cell; Fas-Fas ligand pathway; apoptosis


This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
T. R. McMillan, B. B. Moore, J. B. Weinberg, K. M. Vannella, W. B. Fields, P. J. Christensen, L. F. van Dyk, and G. B. Toews
Exacerbation of Established Pulmonary Fibrosis in a Murine Model by Gammaherpesvirus
Am. J. Respir. Crit. Care Med., April 1, 2008; 177(7): 771 - 780.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
M. Yamada, K. Kuwano, T. Maeyama, M. Yoshimi, N. Hamada, J. Fukumoto, K. Egashira, K. Hiasa, K. Takayama, and Y. Nakanishi
Gene transfer of soluble transforming growth factor type II receptor by in vivo electroporation attenuates lung injury and fibrosis
J. Clin. Pathol., August 1, 2007; 60(8): 916 - 920.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
L. R. Young, R. Pasula, P. M. Gulleman, G. H. Deutsch, and F. X. McCormack
Susceptibility of Hermansky-Pudlak Mice to Bleomycin-Induced Type II Cell Apoptosis and Fibrosis
Am. J. Respir. Cell Mol. Biol., July 1, 2007; 37(1): 67 - 74.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Inoshima, K. Kuwano, N. Hamada, N. Hagimoto, M. Yoshimi, T. Maeyama, A. Takeshita, S. Kitamoto, K. Egashira, and N. Hara
Anti-monocyte chemoattractant protein-1 gene therapy attenuates pulmonary fibrosis in mice
Am J Physiol Lung Cell Mol Physiol, May 1, 2004; 286(5): L1038 - L1044.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Inoshima, K. Kuwano, N. Hamada, M. Yoshimi, T. Maeyama, N. Hagimoto, Y. Nakanishi, and N. Hara
Induction of CDK inhibitor p21 gene as a new therapeutic strategy against pulmonary fibrosis
Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L727 - L733.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. J. Segel, G. Izbicki, P. Y. Cohen, R. Or, T. G. Christensen, S. B. Wallach-Dayan, and R. Breuer
Role of interferon-{gamma} in the evolution of murine bleomycin lung fibrosis
Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1255 - L1262.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Huaux, T. Liu, B. McGarry, M. Ullenbruch, and S. H. Phan
Dual Roles of IL-4 in Lung Injury and Fibrosis
J. Immunol., February 15, 2003; 170(4): 2083 - 2092.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Huaux, M. Arras, D. Tomasi, V. Barbarin, M. Delos, J.-P. Coutelier, A. Vink, S. H. Phan, J.-C. Renauld, and D. Lison
A Profibrotic Function of IL-12p40 in Experimental Pulmonary Fibrosis
J. Immunol., September 1, 2002; 169(5): 2653 - 2661.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online