AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 280: L1225-L1232, 2001;
1040-0605/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lahiri, T.
Right arrow Articles by Shore, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lahiri, T.
Right arrow Articles by Shore, S. A.
Vol. 280, Issue 6, L1225-L1232, June 2001

Interleukin-6 family cytokines: signaling and effects in human airway smooth muscle cells

Thomas Lahiri1, Johanne D. Laporte1, Paul E. Moore1, Reynold A. Panettieri Jr.2, and Stephanie A. Shore1

1 Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115; and 2 Pulmonary and Critical Care Division, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104

Interleukin (IL)-1beta induces cyclooxygenase (COX)-2 expression and prostanoid formation in cultured human airway smooth muscle (HASM) cells. In other cell types, IL-6 family cytokines induce COX-2 or augment IL-1beta -induced COX-2 expression. The purpose of this study was to determine whether IL-6 family cytokines were involved in COX-2 expression in HASM cells. RT-PCR was used to demonstrate that the necessary receptor components for IL-6-type cytokine binding are expressed in HASM cells. IL-6 and oncostatin M (OSM) each caused a dose-dependent phosphorylation of signal transducer and activator of transcription-3, whereas IL-11 did not. IL-6, IL-11, and OSM alone had no effect on COX-2 expression. However, OSM caused dose-dependent augmentation of COX-2 expression and prostaglandin (PG) E2 release induced by IL-1beta . In contrast, IL-6 and IL-11 did not alter IL-1beta -induced COX-2 expression. IL-6 did increase IL-1beta -induced PGE2 formation in unstimulated cells but not in cells stimulated with arachidonic acid (AA; 10-5 M), suggesting that IL-6 effects were mediated at the level of AA release. Our results indicate that IL-6 and OSM are capable of inducing signaling in HASM cells. In addition, OSM and IL-1beta synergistically cause COX-2 expression and PGE2 release.

oncostatin; cyclooxygenase; prostaglandin; signal transducer and activator of transcription


This article has been cited by other articles:


Home page
 Reproductive SciencesHome page
N. Engineer, S. R. Sooranna, Z. Liang, P. R. Bennett, and M. R. Johnson
Influence of Extracellular Matrix on Cytokine Stimulated Pro-labour Gene Expression in Human Uterine Myocytes
Reproductive Sciences, November 1, 2008; 15(9): 950 - 960.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Demyanets, C. Kaun, K. Rychli, G. Rega, S. Pfaffenberger, T. Afonyushkin, V. N. Bochkov, G. Maurer, K. Huber, and J. Wojta
The inflammatory cytokine oncostatin M induces PAI-1 in human vascular smooth muscle cells in vitro via PI 3-kinase and ERK1/2-dependent pathways
Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1962 - H1968.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. J. Ammit, L. M. Moir, B. G. Oliver, J. M. Hughes, H. Alkhouri, Q. Ge, J. K. Burgess, J. L. Black, and M. Roth
Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L199 - L206.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Nishida, S. Tsuji, A. Kimura, M. Tsujii, S. Ishii, T. Yoshio, S. Shinzaki, S. Egawa, T. Irie, M. Yasumaru, et al.
Endothelin-1, an ulcer inducer, promotes gastric ulcer healing via mobilizing gastric myofibroblasts and stimulates production of stroma-derived factors
Am J Physiol Gastrointest Liver Physiol, May 1, 2006; 290(5): G1041 - G1050.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. M. Queen, R. E. Ryan, R. G. Holzer, C. R. Keller-Peck, and C. L. Jorcyk
Breast Cancer Cells Stimulate Neutrophils to Produce Oncostatin M: Potential Implications for Tumor Progression
Cancer Res., October 1, 2005; 65(19): 8896 - 8904.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. S. Faffe, L. Flynt, M. Mellema, T. R. Whitehead, K. Bourgeois, R. A. Panettieri Jr., E. S. Silverman, and S. A. Shore
Oncostatin M causes VEGF release from human airway smooth muscle: synergy with IL-1{beta}
Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1040 - L1048.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. S. Faffe, T. Whitehead, P. E. Moore, S. Baraldo, L. Flynt, K. Bourgeois, R. A. Panettieri, and S. A. Shore
IL-13 and IL-4 promote TARC release in human airway smooth muscle cells: role of IL-4 receptor genotype
Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L907 - L914.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. R. Simon, S. Takahashi, M. Severgnini, B. L. Fanburg, and B. H. Cochran
Role of the JAK-STAT pathway in PDGF-stimulated proliferation of human airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1296 - L1304.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online