AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 280: L1258-L1265, 2001;
1040-0605/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (45)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fukuda, N.
Right arrow Articles by Matthay, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fukuda, N.
Right arrow Articles by Matthay, M. A.
Vol. 280, Issue 6, L1258-L1265, June 2001

Mechanisms of TNF-alpha stimulation of amiloride-sensitive sodium transport across alveolar epithelium

Norimasa Fukuda1, Christian Jayr1, Ahmed Lazrak2, Yibing Wang1, Rudolf Lucas3, Sadis Matalon2, and Michael A. Matthay1

1 Cardiovascular Research Institute, University of California, San Francisco, California 94143-0130; 2 Department of Anesthesiology, Physiology, and Biophysics, University of Alabama, Birmingham, Alabama 35223; and 3 Department of Biological Chemistry, Weizmann Institute of Science, 76100 Rehovot, Israel

Because tumor necrosis factor (TNF)-alpha can upregulate alveolar fluid clearance (AFC) in pneumonia or septic peritonitis, the mechanisms responsible for the TNF-alpha -mediated increase in epithelial fluid transport were studied. In rats, 5 µg of TNF-alpha in the alveolar instillate increased AFC by 67%. This increase was inhibited by amiloride but not by propranolol. We also tested a triple-mutant TNF-alpha that is deficient in the lectinlike tip portion of the molecule responsible for its membrane conductance effect; the mutant also has decreased binding affinity to both TNF-alpha receptors. The triple-mutant TNF-alpha did not increase AFC. Perfusion of human A549 cells, patched in the whole cell mode, with TNF-alpha (120 ng/ml) resulted in a sustained increase in Na+ currents from 82 ± 9 to 549 ± 146 pA (P < 0.005; n = 6). The TNF-alpha -elicited Na+ current was inhibited by amiloride, and there was no change when A549 cells were perfused with the triple-mutant TNF-alpha or after preincubation with blocking antibodies to the two TNF-alpha receptors before perfusion with TNF-alpha . In conclusion, although TNF- alpha  can initiate acute inflammation and edema formation in the lung, TNF-alpha can also increase AFC by an amiloride-sensitive, cAMP-independent mechanism that enhances the resolution of alveolar edema in pathological conditions by either binding to its receptors or activating Na+ channels by means of its lectinlike domain.

tumor necrosis factor-alpha ; tumor necrosis factor receptor; patch clamp; alveolar epithelial cell; acute lung injury; pulmonary edema; pneumonia


This article has been cited by other articles:


Home page
 Chem SensesHome page
P. L. Wall and L. P. McCluskey
Rapid Changes in Gustatory Function Induced by Contralateral Nerve Injury and Sodium Depletion
Chem Senses, February 1, 2008; 33(2): 125 - 135.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H.-F. Bao, Z.-R. Zhang, Y.-Y. Liang, J. J. Ma, D. C. Eaton, and H.-P. Ma
Ceramide mediates inhibition of the renal epithelial sodium channel by tumor necrosis factor-{alpha} through protein kinase C
Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1178 - F1186.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. R. Wilson, M. E. Goddard, K. P. O'Dea, S. Choudhury, and M. Takata
Differential roles of p55 and p75 tumor necrosis factor receptors on stretch-induced pulmonary edema in mice
Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L60 - L68.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. A. Matthay and H. G. Folkesson
Reduction of Alveolar Epithelial Ion and Fluid Transport by Inflammatory Mediators
Am. J. Respir. Cell Mol. Biol., March 1, 2007; 36(3): 389 - 389.
[Full Text] [PDF]

Home page
 Int ImmunolHome page
M. Bilej, R. Joskova, R. Van den Bergh, P. Prochazkova, M. Silerova, P. Ameloot, P. De Baetselier, and A. Beschin
An invertebrate TNF functional analogue activates macrophages via lectin-saccharide interaction with ion channels
Int. Immunol., December 1, 2006; 18(12): 1663 - 1670.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Dagenais, R. Frechette, M.-E. Clermont, C. Masse, A. Prive, E. Brochiero, and Y. Berthiaume
Dexamethasone inhibits the action of TNF on ENaC expression and activity
Am J Physiol Lung Cell Mol Physiol, December 1, 2006; 291(6): L1220 - L1231.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
H. G. Folkesson and M. A. Matthay
Alveolar Epithelial Ion and Fluid Transport: Recent Progress
Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 10 - 19.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
I. C. Davis, E. R. Lazarowski, J. M. Hickman-Davis, J. A. Fortenberry, F.-P. Chen, X. Zhao, E. Sorscher, L. M. Graves, W. M. Sullender, and S. Matalon
Leflunomide Prevents Alveolar Fluid Clearance Inhibition by Respiratory Syncytial Virus
Am. J. Respir. Crit. Care Med., March 15, 2006; 173(6): 673 - 682.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. M. Mutlu and J. I. Sznajder
Mechanisms of pulmonary edema clearance
Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L685 - L695.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
G. M. Mutlu, W. J. Koch, and P. Factor
Alveolar Epithelial {beta}2-Adrenergic Receptors: Their Role in Regulation of Alveolar Active Sodium Transport
Am. J. Respir. Crit. Care Med., December 15, 2004; 170(12): 1270 - 1275.
[Full Text] [PDF]

Home page
 PhysiologyHome page
K. Kunzelmann and B. McMorran
First Encounter: How Pathogens Compromise Epithelial Transport
Physiology, October 1, 2004; 19(5): 240 - 244.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Lebowitz, R. S. Edinger, B. An, C. J. Perry, S. Onate, T. R. Kleyman, and J. P. Johnson
I{kappa}B Kinase-{beta} (IKK{beta}) Modulation of Epithelial Sodium Channel Activity
J. Biol. Chem., October 1, 2004; 279(40): 41985 - 41990.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
L. B. Ware
Modulation of Alveolar Fluid Clearance by Acute Inflammation: The Plot Thickens
Am. J. Respir. Crit. Care Med., February 1, 2004; 169(3): 332 - 333.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Dagenais, R. Frechette, Y. Yamagata, T. Yamagata, J.-F. Carmel, M.-E. Clermont, E. Brochiero, C. Masse, and Y. Berthiaume
Downregulation of ENaC activity and expression by TNF-{alpha} in alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L301 - L311.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
Y. Berthiaume
Tumor Necrosis Factor and Lung Edema Clearance: The Tip of the Iceberg?
Am. J. Respir. Crit. Care Med., November 1, 2003; 168(9): 1022 - 1023.
[Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
N. Elia, M. Tapponnier, M. A. Matthay, J. Hamacher, J.-C. Pache, M.-A. Brundler, M. Totsch, P. De Baetselier, L. Fransen, N. Fukuda, et al.
Functional Identification of the Alveolar Edema Reabsorption Activity of Murine Tumor Necrosis Factor-{alpha}
Am. J. Respir. Crit. Care Med., November 1, 2003; 168(9): 1043 - 1050.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
N. C. Chung, B. Illek, J. H. Widdicombe, and H. Fischer
Measurement of Nasal Potential Difference in Mild Asthmatics
Chest, May 1, 2003; 123(5): 1467 - 1471.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Y. Berthiaume, H. G. Folkesson, and M. A. Matthay
Lung Edema Clearance: 20 Years of Progress: Invited Review: Alveolar edema fluid clearance in the injured lung
J Appl Physiol, December 1, 2002; 93(6): 2207 - 2213.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. Sartori, X. Fang, D. W. McGraw, P. Koch, M. E. Snider, H. G. Folkesson, and M. A. Matthay
Lung Edema Clearance: 20 Years of Progress: Selected Contribution: Long-term effects of beta 2-adrenergic receptor stimulation on alveolar fluid clearance in mice
J Appl Physiol, November 1, 2002; 93(5): 1875 - 1880.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. P. McCluskey and D. L. Hill
Sensitive periods for the effect of dietary sodium restriction on intact and denervated taste receptor cells
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2002; 283(5): R1275 - R1284.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. A. Matthay, H. G. Folkesson, and C. Clerici
Lung Epithelial Fluid Transport and the Resolution of Pulmonary Edema
Physiol Rev, July 1, 2002; 82(3): 569 - 600.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. J. Haddad and S. C. Land
Amiloride Blockades Lipopolysaccharide-Induced Proinflammatory Cytokine Biosynthesis in an Ikappa B-alpha /NF-kappa B-Dependent Mechanism . Evidence for the Amplification of an Antiinflammatory Pathway in the Alveolar Epithelium
Am. J. Respir. Cell Mol. Biol., January 1, 2002; 26(1): 114 - 126.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Danahay, H. Atherton, G. Jones, R. J. Bridges, and C. T. Poll
Interleukin-13 induces a hypersecretory ion transport phenotype in human bronchial epithelial cells
Am J Physiol Lung Cell Mol Physiol, February 1, 2002; 282(2): L226 - L236.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online