AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 280: L1266-L1272, 2001;
1040-0605/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gurel, O.
Right arrow Articles by Jobe, A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gurel, O.
Right arrow Articles by Jobe, A. H.
Vol. 280, Issue 6, L1266-L1272, June 2001

Macrophage and type II cell catabolism of SP-A and saturated phosphatidylcholine in mouse lungs

Okyanus Gurel, Machiko Ikegami, Zissis C. Chroneos, and Alan H. Jobe

Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, Ohio 45229

Type II cells and macrophages are the major cells involved in the alveolar clearance and catabolism of surfactant. We measured type II cell and macrophage contributions to the catabolism of saturated phosphatidylcholine and surfactant protein A (SP-A) in mice. We used intratracheally administered SP-A labeled with residualizing 125I-dilactitol-tyramine, radiolabeled dipalmitoylphosphatidylcholine ([3H]DPPC), and its degradation-resistant analog [14C]DPPC-ether. At 15 min and 7, 19, 29, and 48 h after intratracheal injection, the mice were killed; alveolar lavage was then performed to recover macrophages and surfactant. Type II cells and macrophages not recovered by the lavage were subsequently isolated by enzymatic digestion of the lung. Radioactivity was measured in total lung, lavage fluid macrophages, alveolar washes, type II cells, and lung digest macrophages. Approximately equal amounts of 125I-dilactitol-tyramine-SP-A and [14C]DPPC-ether associated with the macrophages (lavage fluid plus lung digest) and type II cells when corrected for the efficiency of type II cell isolation. Eighty percent of the macrophage-associated radiolabel was recovered from lung digest macrophages. We conclude that macrophages and type II cells contribute equally to saturated phosphatidylcholine and SP-A catabolism in mice.

dipalmitoylphosphatidylcholine; cell isolation; residualizing label; surfactant protein A


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
A. Forbes, M. Pickell, M. Foroughian, L.-J. Yao, J. Lewis, and R. Veldhuizen
Alveolar macrophage depletion is associated with increased surfactant pool sizes in adult rats
J Appl Physiol, August 1, 2007; 103(2): 637 - 645.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Akei, J. A. Whitsett, M. Buroker, T. Ninomiya, H. Tatsumi, T. E. Weaver, and M. Ikegami
Surface tension influences cell shape and phagocytosis in alveolar macrophages
Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L572 - L579.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. Jain, C. Dodia, A. B. Fisher, and S. R. Bates
Pathways for clearance of surfactant protein A from the lung
Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L1011 - L1018.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Ikegami, C.-L. Na, T. R. Korfhagen, and J. A. Whitsett
Surfactant protein D influences surfactant ultrastructure and uptake by alveolar type II cells
Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L552 - L561.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. L. Malloy, R. A. W. Veldhuizen, B. A. Thibodeaux, R. J. O'Callaghan, and J. R. Wright
Pseudomonas aeruginosa protease IV degrades surfactant proteins and inhibits surfactant host defense and biophysical functions
Am J Physiol Lung Cell Mol Physiol, February 1, 2005; 288(2): L409 - L418.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Ikegami, R. Dhami, and E. H. Schuchman
Alveolar lipoproteinosis in an acid sphingomyelinase-deficient mouse model of Niemann-Pick disease
Am J Physiol Lung Cell Mol Physiol, March 1, 2003; 284(3): L518 - L525.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. L. H. Poelma, L. J. I. Zimmermann, H. H. Scholten, B. Lachmann, and J. F. van Iwaarden
In vivo and in vitro uptake of surfactant lipids by alveolar type II cells and macrophages
Am J Physiol Lung Cell Mol Physiol, September 1, 2002; 283(3): L648 - L654.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. F. Seymour and J. J. Presneill
Pulmonary Alveolar Proteinosis: Progress in the First 44 Years
Am. J. Respir. Crit. Care Med., July 15, 2002; 166(2): 215 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Ikegami, W. M. Hull, M. Yoshida, S. E. Wert, and J. A. Whitsett
SP-D and GM-CSF regulate surfactant homeostasis via distinct mechanisms
Am J Physiol Lung Cell Mol Physiol, September 1, 2001; 281(3): L697 - L703.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online