AJP - Lung Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 280: L1359-L1369, 2001;
1040-0605/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jourdan, K. B.
Right arrow Articles by Morrell, N. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jourdan, K. B.
Right arrow Articles by Morrell, N. W.
Vol. 280, Issue 6, L1359-L1369, June 2001

Characterization of adenylyl cyclase isoforms in rat peripheral pulmonary arteries

Karen B. Jourdan1, Nicola A. Mason2, Lu Long2, Peter G. Philips2, Martin R. Wilkins2, and Nicholas W. Morrell1

1 Department of Medicine, Addenbrooke's Hospital, University of Cambridge, Cambridge CB2 2QQ; and 2 Section on Clinical Pharmacology, Hammersmith Campus, Imperial College School of Medicine, London W12 0NN, United Kingdom

Activation of adenylyl cyclase (AC), of which there are 10 diversely regulated isoforms, is important in regulating pulmonary vascular tone and remodeling. Immunohistochemistry in rat lungs demonstrated that AC2, AC3, and AC5/6 predominated in vascular and bronchial smooth muscle. Isoforms 1, 4, 7, and 8 localized to the bronchial epithelium. Exposure of animals to hypoxia did not change the pattern of isoform expression. RT-PCR confirmed mRNA expression of AC2, AC3, AC5, and AC6 and demonstrated AC7 and AC8 transcripts in smooth muscle. Western blotting confirmed the presence of AC2, AC3, and AC5/6 proteins. Functional studies provided evidence of cAMP regulation by Ca2+ and protein kinase C-activated but not Gi-inhibited pathways, supporting a role for AC2 and a Ca2+-stimulated isoform, AC8. However, NKH-477, an AC5-selective activator, was more potent than forskolin in elevating cAMP and inhibiting serum-stimulated [3H]thymidine incorporation, supporting the presence of AC5. These studies demonstrate differential expression of AC isoforms in rat lungs and provide evidence that AC2, AC5, and AC8 are functionally important in cAMP regulation and growth pathways in pulmonary artery myocytes.

vascular smooth muscle; pulmonary hypertension; hypoxia; adenosine 3',5'-cyclic monophosphate; proliferation


This article has been cited by other articles:


Home page
 CirculationHome page
L. Long, A. Crosby, X. Yang, M. Southwood, P. D. Upton, D.-K. Kim, and N. W. Morrell
Altered Bone Morphogenetic Protein and Transforming Growth Factor-{beta} Signaling in Rat Models of Pulmonary Hypertension: Potential for Activin Receptor-Like Kinase-5 Inhibition in Prevention and Progression of Disease
Circulation, February 3, 2009; 119(4): 566 - 576.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. Matsumoto, K. Wakabayashi, T. Kobayashi, and K. Kamata
Functional changes in adenylyl cyclases and associated decreases in relaxation responses in mesenteric arteries from diabetic rats
Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H2234 - H2243.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Sobolewski, K. B. Jourdan, P. D. Upton, L. Long, and N. W. Morrell
Mechanism of cicaprost-induced desensitization in rat pulmonary artery smooth muscle cells involves a PKA-mediated inhibition of adenylyl cyclase
Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L352 - L359.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
C. Zeng, D. Wang, Z. Yang, Z. Wang, L. D. Asico, C. S. Wilcox, G. M. Eisner, W. J. Welch, R. A. Felder, and P. A. Jose
Dopamine D1 Receptor Augmentation of D3 Receptor Action in Rat Aortic or Mesenteric Vascular Smooth Muscles
Hypertension, March 1, 2004; 43(3): 673 - 679.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
H. El-Haroun, D. Bradbury, A. Clayton, and A. J. Knox
Interleukin-1{beta}, Transforming Growth Factor-{beta}1, and Bradykinin Attenuate Cyclic AMP Production by Human Pulmonary Artery Smooth Muscle Cells in Response to Prostacyclin Analogues and Prostaglandin E2 by Cyclooxygenase-2 Induction and Downregulation of Adenylyl Cyclase Isoforms 1, 2, and 4
Circ. Res., February 20, 2004; 94(3): 353 - 361.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
D. L. Cioffi, T. M. Moore, J. Schaack, J. R. Creighton, D. M.F. Cooper, and T. Stevens
Dominant regulation of interendothelial cell gap formation by calcium-inhibited type 6 adenylyl cyclase
J. Cell Biol., June 24, 2002; 157(7): 1267 - 1278.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. T. Chaytor, H. J. Taylor, and T. M. Griffith
Gap junction-dependent and -independent EDHF-type relaxations may involve smooth muscle cAMP accumulation
Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1548 - H1555.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online