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Am J Physiol Lung Cell Mol Physiol 281: L231-L242, 2001;
1040-0605/01 $5.00
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Vol. 281, Issue 1, L231-L242, July 2001

Surfactant protein interactions with neutral and acidic phospholipid films

Karina Rodriguez-Capote1,2, Kaushik Nag1, Samuel Schürch3, and Fred Possmayer1

1 Departments of Obstetrics and Gynaecology and Biochemistry, University of Western Ontario, London, Ontario N6A 5A5; 3 Departments of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada T2N 4N1; and 2 Departamento de Bioquimica, Instituto Superior de Ciencias Médicas de la Habana-Instituto de Ciencias Básicas y Preclínicas Victoria de Giron, Havana, Cuba

The captive bubble tensiometer was employed to study interactions of phospholipid (PL) mixtures of dipalmitoylphosphatidylcholine (DPPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) or 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (POPG) at 50 µg/ml with physiological levels of the surfactant protein (SP) A SP-B, and SP-C alone and in combination at 37°C. All surfactant proteins enhanced lipid adsorption to equilibrium surface tension (gamma ), with SP-C being most effective. Kinetics were consistent with the presence of two adsorption phases. Under the conditions employed, SP-A did not affect the rate of film formation in the presence of SP-B or SP-C. Little difference in gamma min was observed between the acidic POPG and the neutral POPC systems with SP-B or SP-C with and without SP-A. However, gamma max was lower with the acidic POPG system during dynamic, but not during quasi-static, cycling. Considerably lower compression ratios were required to generate low gamma min values with SP-B than SP-C. DPPC-POPG-SP-B was superior to the neutral POPC-SP-B system. Although SP-A had little effect on film formation with SP-B, surface activity during compression was enhanced with both PL systems. In the presence of SP-C, lower compression ratios were required with the acidic system, and with this mixture, SP-A addition adversely affected surface activity. The results suggest specific interactions between SP-B and phosphatidylglycerol, and between SP-B and SP-A. These observations are consistent with the presence of a surface-associated surfactant reservoir which is involved in generating low gamma  during film compression and lipid respreading during film expansion.

acute lung injury; acute respiratory distress syndrome; captive bubble tensiometer; dipalmitoylphosphatidylcholine; lipid monolayer; phosphatidylglycerol; phosphatidylcholine; respiratory distress syndrome; surface area cycling; surface tension; synergism


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