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Am J Physiol Lung Cell Mol Physiol 281: L704-L712, 2001;
1040-0605/01 $5.00
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Vol. 281, Issue 3, L704-L712, September 2001

Persistent mucin glycoprotein alterations in equine recurrent airway obstruction

A. M. Jefcoat1, J. A. Hotchkiss2, V. Gerber1, J. R. Harkema2, C. B. Basbaum3, and N. E. Robinson1

1 Department of Large Animal Clinical Sciences and 2 Department of Veterinary Pathology, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan 48824; and 3 Department of Anatomy, School of Medicine, University of California, San Francisco, California 94143

Horses with the episodic asthmalike condition of recurrent airway obstruction (RAO) have bouts of inflammation and bronchoconstriction associated with indoor housing. To assess the potential differences in airway secretions between RAO-affected and control horses, methods to quantify mucus secretions were developed and applied to bronchoalveolar lavage fluid. The relative difference in the amount of mucin glycoproteins between control and RAO-affected horses was assessed with a carbohydrate side chain-specific monoclonal antibody (4E4) in an enzyme-linked immunosorbent assay and by carbohydrate-specific enzyme-linked lectin assays. Significantly increased levels of 4E4-immunoreactive glycoprotein and the mucin-associated carbohydrates fucose (alpha -1,2 linkage) and N-acetylglucosamine were detected in RAO-affected horses in acute disease. RAO-affected horses in remission maintained significantly elevated levels of alpha -1,2-fucose and N-acetylglucosamine, whereas the 4E4-immunoreactive glycoprotein levels displayed a trend toward an increase over control levels. These results indicated that persistent changes in the quantity and/or quality of mucus glycoproteins occurred in the RAO-affected horses.

bronchoalveolar lavage fluid; O-linked mucin glycoproteins; oligosaccharide side chains; lectins


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