AJP - Lung Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 281: L887-L894, 2001;
1040-0605/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (27)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krick, S.
Right arrow Articles by Yuan, J. X.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krick, S.
Right arrow Articles by Yuan, J. X.-J.
Vol. 281, Issue 4, L887-L894, October 2001

Augmented K+ currents and mitochondrial membrane depolarization in pulmonary artery myocyte apoptosis

Stefanie Krick, Oleksandr Platoshyn, Sharon S. McDaniel, Lewis J. Rubin, and Jason X.-J. Yuan

Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of California School of Medicine, San Diego, California 92103

The balance between apoptosis and proliferation in pulmonary artery smooth muscle cells (PASMCs) is important in maintaining normal pulmonary vascular structure. Activity of voltage-gated K+ (KV) channels has been demonstrated to regulate cell apoptosis and proliferation. Treatment of PASMCs with staurosporine (ST) induced apoptosis in PASMCs, augmented KV current [IK(V)], and induced mitochondrial membrane depolarization. High K+ (40 mM) negligibly affected the ST-induced mitochondrial membrane depolarization but inhibited the ST-induced IK(V) increase and apoptosis. Blockade of KV channels with 4-aminopyridine diminished IK(V) and markedly decreased the ST-mediated apoptosis. Furthermore, the ST-induced apoptosis was preceded by the increase in IK(V). These results indicate that ST induces PASMC apoptosis by activation of plasmalemmal KV channels and mitochondrial membrane depolarization. The increased IK(V) would result in an apoptotic volume decrease due to a loss of cytosolic K+ and induce apoptosis. The mitochondrial membrane depolarization would cause cytochrome c release, activate the cytosolic caspases, and induce apoptosis. Inhibition of KV channels would thus attenuate PASMC apoptosis.

apoptosis; voltage-gated potassium channels; potassium loss; caspases; mitochondrial membrane potential depolarization


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
C. V. Remillard, D. D. Tigno, O. Platoshyn, E. D. Burg, E. E. Brevnova, D. Conger, A. Nicholson, B. K. Rana, R. N. Channick, L. J. Rubin, et al.
Function of Kv1.5 channels and genetic variations of KCNA5 in patients with idiopathic pulmonary arterial hypertension
Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1837 - C1853.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Fantozzi, O. Platoshyn, A. H. Wong, S. Zhang, C. V. Remillard, M. R. Furtado, O. V. Petrauskene, and J. X.-J. Yuan
Bone morphogenetic protein-2 upregulates expression and function of voltage-gated K+ channels in human pulmonary artery smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L993 - L1004.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
S. Bonnet, E. D. Michelakis, C. J. Porter, M. A. Andrade-Navarro, B. Thebaud, S. Bonnet, A. Haromy, G. Harry, R. Moudgil, M. S. McMurtry, et al.
An Abnormal Mitochondrial-Hypoxia Inducible Factor-1{alpha}-Kv Channel Pathway Disrupts Oxygen Sensing and Triggers Pulmonary Arterial Hypertension in Fawn Hooded Rats: Similarities to Human Pulmonary Arterial Hypertension
Circulation, June 6, 2006; 113(22): 2630 - 2641.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. F. Stowe, M. Aldakkak, A. K. S. Camara, M. L. Riess, A. Heinen, S. G. Varadarajan, and M.-T. Jiang
Cardiac mitochondrial preconditioning by Big Ca2+-sensitive K+ channel opening requires superoxide radical generation
Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H434 - H440.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
E. K. Weir, J. Lopez-Barneo, K. J. Buckler, and S. L. Archer
Acute Oxygen-Sensing Mechanisms.
N. Engl. J. Med., November 10, 2005; 353(19): 2042 - 2055.
[Full Text] [PDF]

Home page
 CirculationHome page
J. H. Newman, B. L. Fanburg, S. L. Archer, D. B. Badesch, R. J. Barst, J. G.N. Garcia, P. N. Kao, J. A. Knowles, J. E. Loyd, M. D. McGoon, et al.
Pulmonary Arterial Hypertension: Future Directions: Report of a National Heart, Lung and Blood Institute/Office of Rare Diseases Workshop
Circulation, June 22, 2004; 109(24): 2947 - 2952.
[Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
L. J. Rubin and N. Galie
Pulmonary arterial hypertension: a look to the future
J. Am. Coll. Cardiol., June 16, 2004; 43(12_Suppl_S): 89S - 90S.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. V. Remillard and J. X.-J. Yuan
Activation of K+ channels: an essential pathway in programmed cell death
Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L49 - L67.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
O. Platoshyn, S. Zhang, S. S. McDaniel, and J. X.-J. Yuan
Cytochrome c activates K+ channels before inducing apoptosis
Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1298 - C1305.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online