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Am J Physiol Lung Cell Mol Physiol 281: L1058-L1067, 2001;
1040-0605/01 $5.00
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Vol. 281, Issue 5, L1058-L1067, November 2001

ET-1 stimulates pulmonary arterial smooth muscle cell proliferation via induction of reactive oxygen species

Stephen Wedgwood1, Robert W. Dettman1, and Stephen M. Black1,2

Departments of 1 Pediatrics and 2 Molecular Pharmacology, Northwestern University Medical School, Chicago, Illinois 60611-3008

Recent studies implicate reactive oxygen species (ROS) such as superoxide anions and H2O2 in the proliferation of systemic vascular smooth muscle cells (SMCs). However, the role of ROS in SMC proliferation within the pulmonary circulation remains unclear. We investigated the effects of endothelin-1 (ET-1), a potential SMC mitogen, on ROS production and proliferation of fetal pulmonary artery SMCs (FPASMCs). Exposure to ET-1 resulted in increases in superoxide production and viable FPASMCs after 72 h. These increases were prevented by pretreatment with PD-156707. Treatment with pertussis toxin blocked the effects of ET-1, whereas cholera toxin stimulated superoxide production and increased viable cell numbers even in the absence of ET-1. Wortmannin, LY-294002, diphenyleneiodonium (DPI), 4-(2-aminoethyl)benzenesulfonyl fluoride, and apocynin also prevented the ET-1-mediated increases in superoxide production and viable cell numbers. Exposure to H2O2 or diethyldithiocarbamate increased viable cell number by 37% and 50%, respectively. Conversely, ascorbic acid and DPI decreased viable cell number, which appeared to be due to an increase in programmed cell death. Our data suggest that ET-1 exerts a mitogenic effect on FPASMCs via an increase in ROS production and that antioxidants can block this effect via induction of apoptosis. Antioxidant treatment may therefore represent a potential therapy for pulmonary vascular diseases.

endothelin-1; free radicals; mitogenesis; hypertension; apoptosis


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