AJP - Lung Columbus Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 281: L1130-L1137, 2001;
1040-0605/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grunstein, M. M.
Right arrow Articles by Chuang, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grunstein, M. M.
Right arrow Articles by Chuang, S.
Vol. 281, Issue 5, L1130-L1137, November 2001

Autocrine signaling by IL-10 mediates altered responsiveness of atopic sensitized airway smooth muscle

M. M. Grunstein, H. Hakonarson, J. Leiter, M. Chen, R. Whelan, J. S. Grunstein, and S. Chuang

Division of Pulmonary Medicine, Joseph Stokes, Jr. Research Institute, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104

To elucidate the role and mechanism of action of interleukin (IL)-10 in regulating airway smooth muscle (ASM) responsiveness in the atopic asthmatic state, isolated rabbit tracheal ASM segments were passively sensitized with serum from atopic asthmatic patients or nonatopic nonasthmatic (control) subjects in both the absence and presence of an anti-IL-10 receptor blocking antibody (Ab). Relative to control ASM, atopic asthmatic serum-sensitized tissues exhibited enhanced isometric constrictor responses to administered acetylcholine and attenuated the relaxation responses to isoproterenol. These proasthmatic effects were prevented in atopic asthmatic serum-sensitized ASM that was pretreated with anti-IL-10 receptor Ab. In complementary experiments, exposure of cultured human ASM cells to atopic asthmatic serum induced upregulated expression of IL-10 mRNA. Moreover, extended studies demonstrated that 1) exogenous IL-10 administration to naive ASM elicited augmented contractility to acetylcholine and impaired relaxation to isoproterenol, 2) these effects of IL-10 were prevented by pretreating the tissues with an IL-5 receptor Ab, and 3) IL-10 administration induced upregulated mRNA expression and release of IL-5 protein from cultured ASM cells. Collectively, these findings provide new evidence demonstrating that the altered responsiveness of atopic asthmatic serum-sensitized ASM is largely attributed to activation of an intrinsic T helper type 2-type autocrine mechanism involving IL-10-mediated release and the action of IL-5 in the sensitized ASM itself.

interleukin; T helper type 2 cytokines; signal transduction; asthma


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. H. Kim, D. Jain, O. Tliba, B. Yang, W. F. Jester Jr., R. A. Panettieri Jr., Y. Amrani, and E. Pure
TGF-{beta} potentiates airway smooth muscle responsiveness to bradykinin
Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L511 - L520.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. T. Ameredes, J. M. Sethi, H.-L. Liu, A. M. K. Choi, and W. J. Calhoun
Enhanced nitric oxide production associated with airway hyporesponsiveness in the absence of IL-10
Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L868 - L873.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
D. P. Ennis, J. P. Cassidy, and B. P. Mahon
Acellular Pertussis Vaccine Protects against Exacerbation of Allergic Asthma Due to Bordetella pertussis in a Murine Model
Clin. Vaccine Immunol., March 1, 2005; 12(3): 409 - 417.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
Z.-H. Cui, A. Joetham, M. K. Aydintug, Y.-S. Hahn, W. K. Born, and E. W. Gelfand
Reversal of Allergic Airway Hyperreactivity after Long-term Allergen Challenge Depends on {gamma}{delta} T Cells
Am. J. Respir. Crit. Care Med., December 1, 2003; 168(11): 1324 - 1332.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online