AJP - Lung Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 281: L1350-L1360, 2001;
1040-0605/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coppock, E. A.
Right arrow Articles by Tamkun, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coppock, E. A.
Right arrow Articles by Tamkun, M. M.
Vol. 281, Issue 6, L1350-L1360, December 2001

EDITORIAL FOCUS
Differential expression of KV channel alpha - and beta -subunits in the bovine pulmonary arterial circulation

Elizabeth A. Coppock and Michael M. Tamkun

Department of Physiology and Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523

Resistance pulmonary arteries constrict in response to hypoxia, whereas conduit pulmonary arteries typically do not respond or dilate slightly. One proposed mechanism for this differential response is the variable expression of pulmonary arterial smooth muscle cell voltage-gated K+ (KV) channel subunits (Kv1.2, Kv2.1, Kv1.5, and Kv3.1b) shown to be O2 sensitive in heterologous expression systems. In this study, immunoblotting and immunohistochemistry were used to examine the expression of KV channel alpha - and beta -subunits in the bovine pulmonary arterial circulation to determine whether differential KV channel subunit distribution is responsible for the distinct sensitivities of pulmonary arteries to hypoxia. Surprisingly, there was little difference in the expression levels of Kv1.2, Kv1.5, and Kv2.1 between conduit and resistance pulmonary arteries. In contrast, expression of the Kv3.1b alpha -subunit and Kvbeta .1, Kvbeta 1.2, and Kvbeta 1.3 accessory subunits dramatically increased along the pulmonary arterial tree. The differential expression of all the beta -subunits but of only one of the putative O2-sensitive alpha -subunits suggests that the alpha -subunits alone are not the O2 sensors but further implicates the auxiliary beta -subunits in pulmonary arterial O2 sensing.

pulmonary artery; oxygen sensor; smooth muscle; voltage-gated potassium channel localization; voltage-gated potassium beta -subunit


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. Lu, J. Wang, L. A. Shimoda, and J. T. Sylvester
Differences in STIM1 and TRPC expression in proximal and distal pulmonary arterial smooth muscle are associated with differences in Ca2+ responses to hypoxia
Am J Physiol Lung Cell Mol Physiol, July 1, 2008; 295(1): L104 - L113.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
O. Platoshyn, Y. Yu, E. A Ko, C. V. Remillard, and J. X.-J. Yuan
Heterogeneity of hypoxia-mediated decrease in IK(V) and increase in [Ca2+]cyt in pulmonary artery smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L402 - L416.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
O. Platoshyn, E. E. Brevnova, E. D. Burg, Y. Yu, C. V. Remillard, and J. X.-J. Yuan
Acute hypoxia selectively inhibits KCNA5 channels in pulmonary artery smooth muscle cells
Am J Physiol Cell Physiol, March 1, 2006; 290(3): C907 - C916.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Wang, L. Weigand, W. Wang, J. T. Sylvester, and L. A. Shimoda
Chronic hypoxia inhibits Kv channel gene expression in rat distal pulmonary artery
Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1049 - L1058.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
F. Plane, R. Johnson, P. Kerr, W. Wiehler, K. Thorneloe, K. Ishii, T. Chen, and W. Cole
Heteromultimeric Kv1 Channels Contribute to Myogenic Control of Arterial Diameter
Circ. Res., February 4, 2005; 96(2): 216 - 224.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
O. Platoshyn, C. V. Remillard, I. Fantozzi, M. Mandegar, T. T. Sison, S. Zhang, E. Burg, and J. X.-J. Yuan
Diversity of voltage-dependent K+ channels in human pulmonary artery smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L226 - L238.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. Lopez-Barneo, R. del Toro, K. L. Levitsky, M. D. Chiara, and P. Ortega-Saenz
Regulation of oxygen sensing by ion channels
J Appl Physiol, March 1, 2004; 96(3): 1187 - 1195.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. M. Littler, K. G. Morris Jr., K. A. Fagan, I. F. McMurtry, R. O. Messing, and E. C. Dempsey
Protein kinase C-epsilon -null mice have decreased hypoxic pulmonary vasoconstriction
Am J Physiol Heart Circ Physiol, April 1, 2003; 284(4): H1321 - H1331.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Conforti, M. Petrovic, D. Mohammad, S. Lee, Q. Ma, S. Barone, and A. H. Filipovich
Hypoxia Regulates Expression and Activity of Kv1.3 Channels in T Lymphocytes: A Possible Role in T Cell Proliferation
J. Immunol., January 15, 2003; 170(2): 695 - 702.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
T. Gonzalez, R. Navarro-Polanco, C. Arias, R. Caballero, I. Moreno, E. Delpon, J. Tamargo, M. M. Tamkun, and C. Valenzuela
Assembly with the Kvbeta 1.3 Subunit Modulates Drug Block of hKv1.5 Channels
Mol. Pharmacol., December 1, 2002; 62(6): 1456 - 1463.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. X.-J. Yuan
Oxygen-sensitive K+ channel(s): where and what?
Am J Physiol Lung Cell Mol Physiol, December 1, 2001; 281(6): L1345 - L1349.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online