AJP - Lung Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 282: L115-L123, 2002;
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (31)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Howat, W. J.
Right arrow Articles by Lackie, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Howat, W. J.
Right arrow Articles by Lackie, P. M.
Vol. 282, Issue 1, L115-L123, January 2002

TGF-beta isoform release and activation during in vitro bronchial epithelial wound repair

William J. Howat, Stephen T. Holgate, and Peter M. Lackie

Division of Respiratory, Cell and Molecular Biology, School of Medicine, University of Southampton, Southampton General Hospital, Southampton, SO16 6YD, United Kingdom

Restitution of an epithelial layer after environmental or biological damage is important to maintain the normal function of the respiratory tract. We have investigated the role of transforming growth factor (TGF)-beta isoforms in the repair of layers of 16HBE 14o- bronchial epithelial-derived cells after damage by multiple scoring. ELISA showed that both latent TGF-beta 1 and TGF-beta 2 were converted to their active forms 2 h after wounding. Time-lapse microscopy showed that the addition of TGF-beta 1, but not TGF-beta 2, progressively increased the rate of migration of damaged monolayers at concentrations down to 250 pg/ml. This increase was blocked by addition of a neutralizing TGF-beta 1 antibody. Phase-contrast microscopy and inhibition of proliferation with mitomycin C showed that proliferation was not required for migration. These results demonstrate that conversion of latent to active TGF-beta 1 and TGF-beta 2 during in vitro epithelial wound repair occurs quickly and that TGF-beta 1 speeds epithelial repair. A faster repair may be advantageous in preventing access of environmental agents to the internal milieu of the lung although the production of active TGF-beta molecules may augment subepithelial fibrosis.

transforming growth factor-beta ; 16HBE 14o- bronchial epithelial cell; cell migration; wound healing; primary bronchial epithelial cells


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. E. Olsen, A. E. Liguori, Y. Zong, R. C. Lantz, J. L. Burgess, and S. Boitano
Arsenic upregulates MMP-9 and inhibits wound repair in human airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L293 - L302.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
K. L. McCann and F. Imani
Transforming Growth Factor {beta} Enhances Respiratory Syncytial Virus Replication and Tumor Necrosis Factor Alpha Induction in Human Epithelial Cells
J. Virol., March 15, 2007; 81(6): 2880 - 2886.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. C. Adam, S. T. Holgate, and P. M. Lackie
Epithelial repair is inhibited by an {alpha}1,6-fucose binding lectin
Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L462 - L468.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Kicic, E. N. Sutanto, P. T. Stevens, D. A. Knight, and S. M. Stick
Intrinsic Biochemical and Functional Differences in Bronchial Epithelial Cells of Children with Asthma
Am. J. Respir. Crit. Care Med., November 15, 2006; 174(10): 1110 - 1118.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. Neurohr, S. L. Nishimura, and D. Sheppard
Activation of Transforming Growth Factor-beta by the Integrin {alpha}vbeta8 Delays Epithelial Wound Closure
Am. J. Respir. Cell Mol. Biol., August 1, 2006; 35(2): 252 - 259.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. Lechapt-Zalcman, V. Pruliere-Escabasse, D. Advenier, S. Galiacy, C. Charriere-Bertrand, A. Coste, A. Harf, M.-P. d'Ortho, and E. Escudier
Transforming growth factor-beta1 increases airway wound repair via MMP-2 upregulation: a new pathway for epithelial wound repair?
Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1277 - L1282.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
C. Boxall, S. T. Holgate, and D. E. Davies
The contribution of transforming growth factor-{beta} and epidermal growth factor signalling to airway remodelling in chronic asthma
Eur. Respir. J., January 1, 2006; 27(1): 208 - 229.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. Wenzel
Severe Asthma in Adults
Am. J. Respir. Crit. Care Med., July 15, 2005; 172(2): 149 - 160.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. Farooqui and G. Fenteany
Multiple rows of cells behind an epithelial wound edge extend cryptic lamellipodia to collectively drive cell-sheet movement
J. Cell Sci., January 1, 2005; 118(1): 51 - 63.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. S. Undevia, D. R. Dorscheid, B. A. Marroquin, W. L. Gugliotta, R. Tse, and S. R. White
Smad and p38-MAPK signaling mediates apoptotic effects of transforming growth factor-{beta}1 in human airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L515 - L524.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Kikuchi, J. D. Shively, J. S. Foley, J. M. Drazen, and D. J. Tschumperlin
Differentiation-dependent responsiveness of bronchial epithelial cells to IL-4/13 stimulation
Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L119 - L126.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
S. Wenzel
Severe/Fatal Asthma
Chest, March 1, 2003; 123(2007): 405S - 410S.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online