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Am J Physiol Lung Cell Mol Physiol 282: L83-L90, 2002;
1040-0605/02 $5.00
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Vol. 282, Issue 1, L83-L90, January 2002

Myosin cross-bridge kinetics in airway smooth muscle: a comparative study of humans, rats, and rabbits

Y. Lecarpentier, F.-X. Blanc, S. Salmeron, J.-C. Pourny, D. Chemla, and C. Coirault

Services de Physiologie et de Médecine Interne, Hôpital de Bicêtre, Assistance Publique-Hôpitaux de Paris, Unité de Formation et de Recherche Paris XI, 94275 Le Kremlin-Bicêtre; Laboratoire d'Optique Appliquée-Ecole Nationale Supérieure des Techniques Avancées-Centre National de la Recherche Scientifique-Unité Mixte de Recherche 76 39-Ecole Polytechnique-Institut National de la Santé et de la Recherche Médicale, 91761 Palaiseau, France

To analyze the kinetics and unitary force of cross bridges (CBs) in airway smooth muscle (ASM), we proposed a new formalism of Huxley's equations adapted to nonsarcomeric muscles (Huxley AF. Prog Biophys Biophys Chem 7: 255-318, 1957). These equations were applied to ASM from rabbits, rats, and humans (n = 12/group). We tested the hypothesis that species differences in whole ASM mechanics were related to differences in CB mechanics. We calculated the total CB number per square millimeter at peak isometric tension (Psi  ×109), CB unitary force (Pi ), and the rate constants for CB attachment (f1) and detachment (g1 and g2). Total tension, Psi , and Pi  were significantly higher in rabbits than in humans and rats. Values of Pi  were 8.6 ± 0.1 pN in rabbits, 7.6 ± 0.3 pN in humans, and 7.7 ± 0.2 pN in rats. Values of Psi  were 4.0 ± 0.5 in rabbits, 1.2 ± 0.1 in humans, and 1.9 ± 0.2 in rats; f1 was lower in humans than in rabbits and rats; g2 was higher in rabbits than in rats and in rats than in humans. In conclusion, ASM mechanical behavior of different species was characterized by specific CB kinetics and CB unitary force.

molecular motors; trachea; bronchi


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