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Am J Physiol Lung Cell Mol Physiol 282: L621-L630, 2002. First published October 26, 2001; doi:10.1152/ajplung.00142.2001
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Vol. 282, Issue 4, L621-L630, April 2002

SPECIAL TOPIC
Alveolar Epithelial Ion and Fluid Transport
beta -Adrenoceptor-mediated control of apical membrane conductive properties in fetal distal lung epithelia

A. Collett, S. J. Ramminger, R. E. Olver, and S. M. Wilson

Lung Membrane Transport Group, Tayside Institute of Child Health, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, United Kingdom

Distal lung epithelial cells isolated from fetal rats were cultured (48 h) on permeable supports so that transepithelial ion transport could be quantified electrometrically. Unstimulated cells generated a short-circuit current (Isc) that was inhibited (~80%) by apical amiloride. The current is thus due, predominantly, to the absorption of Na+ from the apical solution. Isoprenaline increased the amiloride-sensitive Isc about twofold. Experiments in which apical membrane Na+ currents were monitored in basolaterally permeabilized cells showed that this was accompanied by a rise in apical Na+ conductance (GNa+). Isoprenaline also increased apical Cl- conductance (GCl-) by activating an anion channel species sensitive to glibenclamide but unaffected by 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). The isoprenaline-evoked changes in GNa+ and GCl- could account for the changes in Isc observed in intact cells. Glibenclamide had no effect upon the isoprenaline-evoked stimulation of Isc or GNa+ demonstrating that the rise in GCl- is not essential to the stimulation of Na+ transport.

alveolar ion transport; Ussing chambers; permeabilized epithelia


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