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Am J Physiol Lung Cell Mol Physiol 282: L1092-L1098, 2002. First published December 21, 2001; doi:10.1152/ajplung.00378.2001
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Vol. 282, Issue 5, L1092-L1098, May 2002

Activity of pulmonary edema fluid interleukin-8 bound to alpha 2-macroglobulin in patients with acute lung injury

Anna K. Kurdowska1, Thomas K. Geiser2, Susanne M. Alden1, Bozena R. Dziadek1, James M. Noble1, Thomas J. Nuckton2, and Michael A. Matthay2

1 Department of Biochemistry, University of Texas Health Center, Tyler, Texas 75708; and 2 School of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143-0130

The formation of alpha 2-macroglobulin (alpha 2-M)/interleukin-8 (IL-8) complexes may influence the biological activity of IL-8 and the quantitative assessment of IL-8 activity. Therefore, in this study, concentrations of free IL-8 and IL-8 complexes with alpha 2-M were measured in pulmonary edema fluid samples from patients with acute lung injury/acute respiratory distress syndrome (ALI/ARDS) and compared with control patients with hydrostatic pulmonary edema. Patients with ALI/ARDS had significantly higher concentrations of alpha 2-M (P < 0.01) as well as alpha 2-M/IL-8 complexes (P < 0.05). Because a substantial amount of IL-8 is complexed to alpha 2-M, standard assays of free IL-8 may significantly underestimate the concentration of biologically active IL-8 in the distal air spaces of patients with ALI/ARDS. Furthermore, IL-8 bound to alpha 2-M retained its biological activity, and this fraction of IL-8 was protected from proteolytic degradation. Thus complex formation may modulate the acute inflammatory process in the lung.

alpha 2-M in pulmonary edema fluid; neutrophils; IL-8 receptors; specific binding


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