AJP - Lung Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 282: L1339-L1348, 2002. First published January 12, 2002; doi:10.1152/ajplung.00392.2001
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/6/L1339    most recent
00392.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ubl, J. J.
Right arrow Articles by Reiser, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ubl, J. J.
Right arrow Articles by Reiser, G.
Vol. 282, Issue 6, L1339-L1348, June 2002

Human bronchial epithelial cells express PAR-2 with different sensitivity to thermolysin

Joachim J. Ubl1, Zoryana V. Grishina1,2, Tatiana K. Sukhomlin1,3, Tobias Welte4, Fariba Sedehizade1, and Georg Reiser1

1 Institut für Neurobiochemie, 4 Abteilung für Pneumologie der Medizinischen Fakultät der Otto-von-Guericke-Universität Magdeburg, 39120 Magdeburg, Germany; 2 A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899 Moscow; and 3 Research Centre of Molecular Diagnostics and Therapy, 113149 Moscow, Russia

Protease-activated receptor-2 (PAR-2) plays a role in inflammatory reactions in airway physiology. Proteases cleaving the extracellular NH2 terminus of receptors activate or inactivate PAR, thus possessing a therapeutic potential. Using RT-PCR and immunocytochemistry, we show PAR-2 in human airway epithelial cell lines human bronchial epithelial (HBE) and A549. Functional expression of PAR-2 was confirmed by Ca2+ imaging studies using the receptor agonist protease trypsin. The effect was abolished by soybean trypsin inhibitor and mimicked by the specific PAR-2 peptide agonist SLIGKV. Amplitude and duration of PAR-2-elicited Ca2+ response in HBE and A549 cells depend on concentration and time of agonist superfusion. The response is partially pertussis toxin (PTX) insensitive, abolished by the phospholipase C inhibitor U-73122, and diminished by the inositol 1,4,5-trisphosphate receptor antagonist 2-aminoethoxydiphenyl borate. Cathepsin G altered neither the resting Ca2+ level nor PAR-2-elicited Ca2+ response. Thermolysin, a prototypic bacterial metalloprotease, induced a dose-dependent Ca2+ response in HBE, but not A549, cells. In both cell lines, thermolysin abolished the response to a subsequent trypsin challenge but not to SLIGKV. Thus different epithelial cell types express different PAR-2 with identical responses to physiological stimuli (trypsin, SLIGKV) but different sensitivity to modifying proteases, such as thermolysin.

calcium signaling; cathepsin G; trypsin; protease-activated receptor-2


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. J. Moraes, R. Martin, J. D. Plumb, E. Vachon, C. M. Cameron, A. Danesh, D. J. Kelvin, W. Ruf, and G. P. Downey
Role of PAR2 in murine pulmonary pseudomonal infection
Am J Physiol Lung Cell Mol Physiol, February 1, 2008; 294(2): L368 - L377.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. Ostrowska, E. Sokolova, and G. Reiser
PAR-2 activation and LPS synergistically enhance inflammatory signaling in airway epithelial cells by raising PAR expression level and interleukin-8 release
Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1208 - L1218.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Chignard and D. Pidard
Neutrophil and Pathogen Proteinases versus Proteinase-Activated Receptor-2 Lung Epithelial Cells: More Terminators than Activators.
Am. J. Respir. Cell Mol. Biol., April 1, 2006; 34(4): 394 - 398.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. Sokolova, Z. Grishina, F. Buhling, T. Welte, and G. Reiser
Protease-activated receptor-1 in human lung fibroblasts mediates a negative feedback downregulation via prostaglandin E2
Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L793 - L802.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
L. A. Espinoza, M. Valikhani, M. J. Cossio, T. Carr, M. Jung, J. Hyde, M. L. Witten, and M. E. Smulson
Altered Expression of {gamma}-Synuclein and Detoxification-Related Genes in Lungs of Rats Exposed to JP-8
Am. J. Respir. Cell Mol. Biol., March 1, 2005; 32(3): 192 - 200.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. S. Lan, G. A. Stewart, R. G. Goldie, and P. J. Henry
Altered expression and in vivo lung function of protease-activated receptors during influenza A virus infection in mice
Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L388 - L398.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Dulon, C. Cande, N. W. Bunnett, M. D. Hollenberg, M. Chignard, and D. Pidard
Proteinase-Activated Receptor-2 and Human Lung Epithelial Cells: Disarming by Neutrophil Serine Proteinases
Am. J. Respir. Cell Mol. Biol., March 1, 2003; 28(3): 339 - 346.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online