AJP - Lung Columbus Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 282: L1382-L1390, 2002. First published February 1, 2002; doi:10.1152/ajplung.00447.2001
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/6/L1382    most recent
00447.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Palestini, P.
Right arrow Articles by Miserocchi, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Palestini, P.
Right arrow Articles by Miserocchi, G.
Vol. 282, Issue 6, L1382-L1390, June 2002

Composition, biophysical properties, and morphometry of plasma membranes in pulmonary interstitial edema

Paola Palestini1, Chiara Calvi1, Elena Conforti1, Laura Botto1, Carla Fenoglio2, and Giuseppe Miserocchi1

1 Department of Experimental, Environmental Medicine and Biotechnology, University of Milano-Bicocca, Monza 20052; and 2 Department of Animal Biology, University of Pavia, Pavia 27100, Italy

We evaluated the changes in plasma membrane composition, biophysical properties, and morphology of pulmonary endothelial cells in anesthetized rabbits receiving 0.5 ml · kg-1 · min-1 saline infusion for 180 min, causing mild interstitial edema. Plasma membrane fractions were obtained from lung homogenates with gradient centrifugation, allowing a sixfold enrichment in caveolin-1. In edematous lungs, cholesterol content and phospholipidic phosphorus increased by 15 and 40%, respectively. These data correlated with morphometric analysis of lungs fixed in situ by vascular perfusion with 2.5% glutaraldehyde, suggesting a relative increase in surface of luminal to interstitial front of the capillary endothelial cells, due to a convoluted luminal profile. In edematous lungs, the fraction of double-bound fatty acids increased in membrane lipids; moreover, the phosphatidylcholine/phosphatidylethanolamine and the cholesterol/phospholipid ratios decreased. These changes were consistent with the increase in fluorescence anisotropy of plasma membrane, indicating an increase in its fluidity. Data suggest that mechanical stimuli elicited by a modest (~4%) increase in extravascular water cause marked changes in plasma membranes that may be of relevance in signal transduction and endothelial cell activation.

rabbit; air-blood barrier; endothelial cells; membrane composition and fluidity


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
R. Daffara, L. Botto, E. Beretta, E. Conforti, A. Faini, P. Palestini, and G. Miserocchi
Endothelial cells as early sensors of pulmonary interstitial edema
J Appl Physiol, October 1, 2004; 97(4): 1575 - 1583.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Bhattacharya
The alveolar water gate
Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L257 - L258.
[Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. Palestini, C. Calvi, E. Conforti, R. Daffara, L. Botto, and G. Miserocchi
Compositional changes in lipid microdomains of air-blood barrier plasma membranes in pulmonary interstitial edema
J Appl Physiol, October 1, 2003; 95(4): 1446 - 1452.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Bhattacharya
Pressure-induced capillary stress failure: is it regulated?
Am J Physiol Lung Cell Mol Physiol, May 1, 2003; 284(5): L701 - L702.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online