AJP - Lung Columbus Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 283: L205-L210, 2002. First published March 1, 2002; doi:10.1152/ajplung.00443.2001
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/1/L205    most recent
00443.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (23)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dobson, A. W.
Right arrow Articles by Gillespie, M. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dobson, A. W.
Right arrow Articles by Gillespie, M. N.
Vol. 283, Issue 1, L205-L210, July 2002

Enhanced mtDNA repair capacity protects pulmonary artery endothelial cells from oxidant-mediated death

Allison W. Dobson1, Valentina Grishko2, Susan P. LeDoux1, Mark R. Kelley3, Glenn L. Wilson1, and Mark N. Gillespie2

Departments of 1 Cell Biology and Neuroscience and 2 Pharmacology, College of Medicine, University of South Alabama, Mobile, Alabama 36688; and 3 Wells Center for Pediatric Research, Indiana University Medical School, Indianapolis, Indiana 46202

In rat cultured pulmonary arterial (PA), microvascular, and venous endothelial cells (ECs), the rate of mitochondrial (mt) DNA repair is predictive of the severity of xanthine oxidase (XO)-induced mtDNA damage and the sensitivity to XO-mediated cell death. To examine the importance of mtDNA damage and repair more directly, we determined the impact of mitochondrial overexpression of the DNA repair enzyme, Ogg1, on XO-induced mtDNA damage and cell death in PAECs. PAECs were transiently transfected with an Ogg1-mitochondrial targeting sequence construct. Mitochondria-selective overexpression of the transgene product was confirmed microscopically by the observation that immunoreactive Ogg1 colocalized with a mitochondria-specific tracer and, with an oligonucleotide cleavage assay, by a selective enhancement of mitochondrial Ogg1 activity. Overexpression of Ogg1 protected against both XO-induced mtDNA damage, determined by quantitative Southern analysis, and cell death as assessed by trypan blue exclusion and MTS assays. These findings show that mtDNA damage is a direct cause of cell death in XO-treated PAECs.

mitochondrial deoxyribonucleic acid; xanthine oxidase; Ogg1; cytotoxicity


This article has been cited by other articles:


Home page
 Evid Based Complement Alternat MedHome page
S. Zhong, Z. Li, L. Huan, and B.-Y. Chen
Neurochemical Mechanism of Electroacupuncture: Anti-injury Effect on Cerebral Function after Focal Cerebral Ischemia in Rats
Evid. Based Complement. Altern. Med., March 1, 2009; 6(1): 51 - 56.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
T. A. Klimova, E. L. Bell, E. H. Shroff, F. D. Weinberg, C. M. Snyder, G. P. Dimri, P. T. Schumacker, G. R. S. Budinger, and N. S. Chandel
Hyperoxia-induced premature senescence requires p53 and pRb, but not mitochondrial matrix ROS
FASEB J, March 1, 2009; 23(3): 783 - 794.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
L. Xie, X. Zhu, Y. Hu, T. Li, Y. Gao, Y. Shi, and S. Tang
Mitochondrial DNA Oxidative Damage Triggering Mitochondrial Dysfunction and Apoptosis in High Glucose-Induced HRECs
Invest. Ophthalmol. Vis. Sci., September 1, 2008; 49(9): 4203 - 4209.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Cai, Y. Xu, R. J. Cooper, M. J. Ferkowicz, J. R. Hartwell, K. E. Pollok, and M. R. Kelley
Mitochondrial Targeting of Human O6-Methylguanine DNA Methyltransferase Protects against Cell Killing by Chemotherapeutic Alkylating Agents
Cancer Res., April 15, 2005; 65(8): 3319 - 3327.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Ruchko, O. Gorodnya, S. P. LeDoux, M. F. Alexeyev, A.-B. Al-Mehdi, and M. N. Gillespie
Mitochondrial DNA damage triggers mitochondrial dysfunction and apoptosis in oxidant-challenged lung endothelial cells
Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L530 - L535.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. I. Rachek, V. I. Grishko, M. F. Alexeyev, V. V. Pastukh, S. P. LeDoux, and G. L. Wilson
Endonuclease III and endonuclease VIII conditionally targeted into mitochondria enhance mitochondrial DNA repair and cell survival following oxidative stress
Nucleic Acids Res., June 15, 2004; 32(10): 3240 - 3247.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. Panduri, S. A. Weitzman, N. S. Chandel, and D. W. Kamp
Mitochondrial-derived free radicals mediate asbestos-induced alveolar epithelial cell apoptosis
Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1220 - L1227.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M.-R. Lee, S.-H. Kim, H.-J. Cho, K.-Y. Lee, A. R. Moon, H. G. Jeong, J.-S. Lee, J.-W. Hyun, M.-H. Chung, and H. J. You
Transcription Factors NF-YA Regulate the Induction of Human OGG1 Following DNA-alkylating Agent Methylmethane Sulfonate (MMS) Treatment
J. Biol. Chem., March 12, 2004; 279(11): 9857 - 9866.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. T. Lightfoot, S. Khov, and H. Ischiropoulos
Transient injury to rat lung mitochondrial DNA after exposure to hyperoxia and inhaled nitric oxide
Am J Physiol Lung Cell Mol Physiol, January 1, 2004; 286(1): L23 - L29.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. I. Rachek, V. I. Grishko, S. I. Musiyenko, M. R. Kelley, S. P. LeDoux, and G. L. Wilson
Conditional Targeting of the DNA Repair Enzyme hOGG1 into Mitochondria
J. Biol. Chem., November 15, 2002; 277(47): 44932 - 44937.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online