| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1 Pediatrics, 2 Internal Medicine, and 4 Microbiology and Immunology and 3 Sealy Center for Molecular Sciences, University of Texas Medical Branch, Galveston, Texas 77555
Airway epithelial cells represent the primary cell target of respiratory syncytial virus (RSV) infection. They actively participate in the lung immune/inflammatory response that follows RSV infection by expressing chemokines, small chemotactic cytokines that recruit and activate leukocytes. Regulated on activation, normal T cell expressed, and presumably secreted (RANTES) is a member of the CC chemokine subfamily and is strongly chemotactic for T lymphocytes, monocytes, basophils, and eosinophils, cell types that are present or activated in the inflammatory infiltrate that follows RSV infection of the lung. RSV infection of airway epithelial cells induces RANTES expression by increasing gene transcription and stabilizing RNA transcripts. The signaling pathway regulating RANTES gene expression after RSV infection has not been determined. In this study, we examined the role of extracellular signal-regulated kinase (ERK) and p38, members of the mitogen-activated protein (MAP) kinase (MAPK) family, in RSV-induced RANTES production. RSV infection of alveolar epithelial cells induced increased phosphorylation and catalytic activity of ERK and the upstream kinases Raf-1 and MAP ERK kinase. Induction of the MAP signaling cascade required a replication-competent virus. RSV infection of alveolar epithelial cells also induced activation of p38 MAPK. Inhibition of ERK and p38 activation significantly reduced RSV-induced RANTES mRNA and protein secretion without affecting RANTES gene transcription or transcription factor activation. These results indicate that the MAPK signaling cascade regulates RANTES production in alveolar epithelial cells through a posttranscriptional mechanism.
respiratory syncytial virus; chemokine; signal transduction; gene regulation; mitogen-activated protein kinase; regulated on activation; normal T cell expressed, and presumably secreted
This article has been cited by other articles:
|
|
 |
|
  J. Eckardt-Michel, M. Lorek, D. Baxmann, T. Grunwald, G. M. Keil, and G. Zimmer The Fusion Protein of Respiratory Syncytial Virus Triggers p53-Dependent Apoptosis J. Virol., April 1, 2008; 82(7): 3236 - 3249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Saez-Rodriguez, A. Goldsipe, J. Muhlich, L. G. Alexopoulos, B. Millard, D. A. Lauffenburger, and P. K. Sorger Flexible informatics for linking experimental data to mathematical models via DataRail Bioinformatics, March 15, 2008; 24(6): 840 - 847. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Proud and C.-W. Chow Role of Viral Infections in Asthma and Chronic Obstructive Pulmonary Disease Am. J. Respir. Cell Mol. Biol., November 1, 2006; 35(5): 513 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakamichi, M. Saiki, M. Sawada, M. Takayama-Ito, Y. Yamamuro, K. Morimoto, and I. Kurane Rabies Virus-Induced Activation of Mitogen-Activated Protein Kinase and NF-{kappa}B Signaling Pathways Regulates Expression of CXC and CC Chemokine Ligands in Microglia J. Virol., September 15, 2005; 79(18): 11801 - 11812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hargett, T. McLean, and S. L. Bachenheimer Herpes Simplex Virus ICP27 Activation of Stress Kinases JNK and p38 J. Virol., July 1, 2005; 79(13): 8348 - 8360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. W. McL. Rixon, G. Brown, J. T. Murray, and R. J. Sugrue The respiratory syncytial virus small hydrophobic protein is phosphorylated via a mitogen-activated protein kinase p38-dependent tyrosine kinase activity during virus infection J. Gen. Virol., February 1, 2005; 86(2): 375 - 384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. I. Wickremasinghe, L. H. Thomas, C. M. O'Kane, J. Uddin, and J. S. Friedland Transcriptional Mechanisms Regulating Alveolar Epithelial Cell-specific CCL5 Secretion in Pulmonary Tuberculosis J. Biol. Chem., June 25, 2004; 279(26): 27199 - 27210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Melchjorsen and S. R. Paludan Induction of RANTES/CCL5 by herpes simplex virus is regulated by nuclear factor {kappa}B and interferon regulatory factor 3 J. Gen. Virol., September 1, 2003; 84(9): 2491 - 2495. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Melchjorsen, L. N. Sorensen, and S. R. Paludan Expression and function of chemokines during viral infections: from molecular mechanisms to in vivo function J. Leukoc. Biol., September 1, 2003; 74(3): 331 - 343. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
