AJP - Lung AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 283: L541-L548, 2002. First published May 3, 2002; doi:10.1152/ajplung.00413.2001
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/3/L541    most recent
00413.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frossard, J. L.
Right arrow Articles by Steer, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frossard, J. L.
Right arrow Articles by Steer, M. L.
Vol. 283, Issue 3, L541-L548, September 2002

In vivo evidence for the role of GM-CSF as a mediator in acute pancreatitis-associated lung injury

Jean Louis Frossard1,2, Ashok K. Saluja1, Nicolas Mach3,4, Hong Sik Lee1, Lakshmi Bhagat1, Antoine Hadenque2, Laura Rubbia-Brandt5, Glenn Dranoff3, and Michael L. Steer1

1 Department of Surgery, Beth Israel Hospital Deaconess Medical Center, Harvard Medical School; 3 Department of Adult Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115; and Divisions of 2 Gastroenterology, 4 Oncology, and 5 Clinical Pathology, Geneva University Hospitals, 1211 Geneva 14, Switzerland

Severe pancreatitis is frequently associated with acute lung injury (ALI) and the respiratory distress syndrome. The role of granulocyte-macrophage colony-stimulating factor (GM-CSF) in mediating the ALI associated with secretagogue-induced experimental pancreatitis was evaluated with GM-CSF knockout mice (GM-CSF -/-). Pancreatitis was induced by hourly (12×) intraperitoneal injection of a supramaximally stimulating dose of the cholecystokinin analog caerulein. The resulting pancreatitis was similar in GM-CSF-sufficient (GM-CSF +/+) control animals and GM-CSF -/- mice. Lung injury, quantitated by measuring lung myeloperoxidase activity (an indicator of neutrophil sequestration), alveolar-capillary permeability, and alveolar membrane thickness was less severe in GM-CSF -/- than in GM-CSF +/+ mice. In GM-CSF +/+ mice, pancreas, lung and serum GM-CSF levels increase during pancreatitis. Lung levels of macrophage inflammatory protein (MIP)-2 are also increased during pancreatitis, but, in this case, the rise is less profound in GM-CSF -/- mice than in GM-CSF +/+ controls. Administration of anti-MIP-2 antibodies was found to reduce the severity of pancreatitis-associated ALI. Our findings indicate that GM-CSF plays a critical role in coupling pancreatitis to ALI and suggest that GM-CSF may act indirectly by regulating the release of other proinflammatory factors including MIP-2.

granulocyte-macrophage colony-stimulating factor; caerulein; inflammation; adult respiratory distress syndrome; adhesion molecules; neutrophils


This article has been cited by other articles:


Home page
 GutHome page
R Sharif, R Dawra, K Wasiluk, P Phillips, V Dudeja, E Kurt-Jones, R Finberg, and A Saluja
Impact of toll-like receptor 4 on the severity of acute pancreatitis and pancreatitis-associated lung injury in mice
Gut, June 1, 2009; 58(6): 813 - 819.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. C. Parker and M. I. Townsley
Evaluation of lung injury in rats and mice
Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L231 - L246.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
B. Ji, X.-q. Chen, D. E. Misek, R. Kuick, S. Hanash, S. Ernst, R. Najarian, and C. D. Logsdon
Pancreatic gene expression during the initiation of acute pancreatitis: identification of EGR-1 as a key regulator
Physiol Genomics, June 24, 2003; 14(1): 59 - 72.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online