AJP - Lung AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 283: L791-L798, 2002. First published June 5, 2002; doi:10.1152/ajplung.00398.2001
1040-0605/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/4/L791    most recent
00398.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Turi, J. L.
Right arrow Articles by Ghio, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Turi, J. L.
Right arrow Articles by Ghio, A. J.
Vol. 283, Issue 4, L791-L798, October 2002

Oxidative stress activates anion exchange protein 2 and AP-1 in airway epithelial cells

Jennifer L. Turi1, Ilona Jaspers2, Lisa A. Dailey3, Michael C. Madden3, Luisa E. Brighton2, Jacqueline D. Carter3, Eva Nozik-Grayck1, Claude A. Piantadosi4, and Andrew J. Ghio3

1 Department of Pediatrics, Duke University Medical Center; 4 Department of Internal Medicine, Duke University Medical Center, Durham 27710; 2 Center for Environmental Medicine and Lung Biology, University of North Carolina, Chapel Hill 27599; and 3 National Health and Environmental Effects Research Laboratory, Office of Research and Development, Environmental Protection Agency, Research Triangle Park, North Carolina 27711

Anion exchange protein 2 (AE2) is a membrane-bound protein that mediates chloride-bicarbonate exchange. In addition to regulating intracellular pH and cell volume, AE2 exports superoxide (O<UP><SUB>2</SUB><SUP>−</SUP></UP>·) to the extracellular matrix in an HCO<UP><SUB>3</SUB><SUP>−</SUP></UP>-dependent process. Given this ability to export O<UP><SUB>2</SUB><SUP>−</SUP></UP>·, we hypothesized that expression of AE2 in the lung is regulated by oxidative stress. AE2 mRNA and protein expression was measured by RT-PCR and Western blot analysis, respectively, in differentiated human bronchial epithelial cells exposed to H2O2 (100 µM). Alterations in in vivo AE2 protein expression were evaluated in lung tissue of rats exposed to 70% O2. The role of transcription factor activator protein (AP)-1 in oxidant regulation of AE2 was evaluated by EMSA and by immunoblotting of nuclear phospho-c-jun. Results show increased AE2 mRNA and protein expression after oxidant exposure. This was preceded by transient increases in DNA binding of AE2-specific AP-1 and phosphorylation of c-jun. This study demonstrates that AE2 expression is regulated by oxidative stress in airway epithelial cells and that this regulation correlates with activation of AP-1.

superoxide; ion transport; gene regulation; activator protein-1


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. J. Ross, L. A. Dailey, L. E. Brighton, and R. B. Devlin
Transcriptional Profiling of Mucociliary Differentiation in Human Airway Epithelial Cells
Am. J. Respir. Cell Mol. Biol., August 1, 2007; 37(2): 169 - 185.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. L. Turi, X. Wang, A. T. McKie, E. Nozik-Grayck, L. B. Mamo, K. Crissman, C. A. Piantadosi, and A. J. Ghio
Duodenal cytochrome b: a novel ferrireductase in airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2006; 291(2): L272 - L280.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
F. Yang, D. J. Haile, X. Wang, L. A. Dailey, J. G. Stonehuerner, and A. J. Ghio
Apical location of ferroportin 1 in airway epithelia and its role in iron detoxification in the lung
Am J Physiol Lung Cell Mol Physiol, July 1, 2005; 289(1): L14 - L23.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. S. Powell, M. M. Wright, and R. M. Jackson
p38mapk and MEK1/2 inhibition contribute to cellular oxidant injury after hypoxia
Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L826 - L833.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online