AJP - Lung AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 284: L290-L297, 2003. First published October 18, 2002; doi:10.1152/ajplung.00197.2002
1040-0605/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/2/L290    most recent
00197.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fujii, T.
Right arrow Articles by Hayashi, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fujii, T.
Right arrow Articles by Hayashi, S.
Vol. 284, Issue 2, L290-L297, February 2003

Adenoviral E1A modulates inflammatory mediator expression by lung epithelial cells exposed to PM10

Takeshi Fujii1, James C. Hogg1, Naoto Keicho1, Renaud Vincent2, Stephan F. Van Eeden1, and Shizu Hayashi1

1 McDonald Research Laboratory and iCAPTURE Center, University of British Columbia, Vancouver, British Columbia V6Z 1Y6; and 2 Environmental Health Directorate, Health Canada, Ottawa, Ontario K1A 0L2, Canada

We examined the hypothesis that ambient particulate matter with a diameter of <10 µm (PM10)-induced lung inflammation is amplified by latent adenovirus infection. Inflammatory mediator expression in response to PM10 exposure was compared between adenovirus E1A-transfected A549 alveolar epithelial cells and cells transfected with control plasmid. Messenger RNA was measured by the RNase protection assay and protein by ELISA or immunocytochemistry. Intercellular adhesion molecule-1 and IL-8 mRNA and protein were increased in E1A-positive cells exposed to 500 µg/ml PM10. Monocyte chemoattractant protein-1 mRNA and protein were unchanged in E1A-positive cells but increased in E1A-negative cells after 100 and 500 µg/ml PM10 exposure. Electrophoretic mobility shift assays showed increased NF-kappa B and decreased specificity protein 1 nuclear binding in E1A-positive cells exposed to PM10. These results indicate that E1A modulates cytokine and adhesion molecule expression in epithelial cells in a manner that could amplify PM10-induced lung inflammation. We suggest that this amplified inflammatory response may contribute to the pathogenesis of exacerbations of chronic obstructive pulmonary disease associated with exposure to particulate matter air pollution.

interleukin-8; monocyte chemoattractant protein-1; intercellular adhesion molecule-1; nuclear factor-kappa B; transcription factor specificity protein 1; particulate matter


This article has been cited by other articles:


Home page
 J. Epidemiol. Community HealthHome page
J.-H. Choi, Q.-S. Xu, S.-Y. Park, J.-H. Kim, S.-S. Hwang, K.-H. Lee, H.-J. Lee, and Y.-C. Hong
Seasonal variation of effect of air pollution on blood pressure
J Epidemiol Community Health, April 1, 2007; 61(4): 314 - 318.
[Abstract] [Full Text] [PDF]

Home page
 ERRHome page
R. Lutter and M. Spiteri
Current perspectives in epithelial cell injury and repair: consequences for epithelial functions
Eur. Respir. Rev., December 1, 2005; 14(97): 126 - 130.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
T. Muller, H. Bayer, D. Myrtek, D. Ferrari, S. Sorichter, M. W. Ziegenhagen, G. Zissel, J. C. Virchow Jr., W. Luttmann, J. Norgauer, et al.
The P2Y14 Receptor of Airway Epithelial Cells: Coupling to Intracellular Ca2+ and IL-8 Secretion
Am. J. Respir. Cell Mol. Biol., December 1, 2005; 33(6): 601 - 609.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online