AJP - Lung Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 284: L316-L323, 2003. First published September 27, 2002; doi:10.1152/ajplung.00228.2002
1040-0605/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/2/L316    most recent
00228.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fike, C. D.
Right arrow Articles by Pfister, S. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fike, C. D.
Right arrow Articles by Pfister, S. L.
Vol. 284, Issue 2, L316-L323, February 2003

Arachidonic acid metabolites and an early stage of pulmonary hypertension in chronically hypoxic newborn pigs

Candice D. Fike, Mark R. Kaplowitz, and Sandra L. Pfister

Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226; and Department of Pediatrics, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157

Our purpose was to determine whether production of arachidonic acid metabolites, particularly cyclooxygenase (COX) metabolites, is altered in 100-400-µm-diameter pulmonary arteries of piglets at an early stage of pulmonary hypertension. Piglets were raised in either room air (control) or hypoxia for 3 days. A cannulated artery technique was used to measure responses of 100-400-µm-diameter pulmonary arteries to arachidonic acid, a prostacyclin analog, or the thromboxane mimetic U46619. Radioimmunoassay was used to determine pulmonary artery production of thromboxane B2 (TxB2) and 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha ), the stable metabolites of thromboxane and prostacyclin, respectively. Assessment of abundances of COX pathway enzymes in pulmonary arteries was determined by immunoblot technique. Arachidonic acid induced less dilation in pulmonary arteries from hypoxic than in pulmonary arteries from control piglets. Pulmonary artery responses to prostacyclin and U46619 were similar for both groups. 6-Keto-PGF1alpha production was reduced, whereas TxB2 production was increased in pulmonary arteries from hypoxic piglets. Abundances of both COX-1 and prostacyclin synthase were reduced, whereas abundances of both COX-2 and thromboxane synthase were unaltered in pulmonary arteries from hypoxic piglets. At least partly due to altered abundances of COX pathway enzymes, a shift in production of arachidonic acid metabolites, away from dilators toward constrictors, may contribute to the early phase of chronic hypoxia-induced pulmonary hypertension in newborn piglets.

cyclooxygenase-1; cyclooxygenase-2; prostacyclin; thromboxane


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. M. Sellers and J. N. Stallone
Sympathy for the devil: the role of thromboxane in the regulation of vascular tone and blood pressure
Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H1978 - H1986.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Hinton, A. Gutsol, and S. Dakshinamurti
Thromboxane hypersensitivity in hypoxic pulmonary artery myocytes: altered TP receptor localization and kinetics
Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L654 - L663.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Hinton, L. Mellow, A. J. Halayko, A. Gutsol, and S. Dakshinamurti
Hypoxia induces hypersensitivity and hyperreactivity to thromboxane receptor agonist in neonatal pulmonary arterial myocytes
Am J Physiol Lung Cell Mol Physiol, February 1, 2006; 290(2): L375 - L384.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C.-F. Lam, T. E. Peterson, A. J. Croatt, K. A. Nath, and Z. S. Katusic
Functional adaptation and remodeling of pulmonary artery in flow-induced pulmonary hypertension
Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2334 - H2341.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Li and J. N. Stallone
Estrogen potentiates vasopressin-induced contraction of female rat aorta by enhancing cyclooxygenase-2 and thromboxane function
Am J Physiol Heart Circ Physiol, October 1, 2005; 289(4): H1542 - H1550.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. D. Fike, Y. Zhang, and M. R. Kaplowitz
Thromboxane inhibition reduces an early stage of chronic hypoxia-induced pulmonary hypertension in piglets
J Appl Physiol, August 1, 2005; 99(2): 670 - 676.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. D. Fike, M. R. Kaplowitz, Y. Zhang, and S. L. Pfister
Cyclooxygenase-2 and an early stage of chronic hypoxia-induced pulmonary hypertension in newborn pigs
J Appl Physiol, March 1, 2005; 98(3): 1111 - 1118.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
S. C. Park, Y. Liu-Stratton, L. C. Medeiros, S. A. Mccune, and M. J. Radin
Effect of Male Sex and Obesity on Platelet Arachidonic Acid in Spontaneous Hypertensive Heart Failure Rats
Experimental Biology and Medicine, July 1, 2004; 229(7): 657 - 664.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. D. Fike, J. L. Aschner, Y. Zhang, and M. R. Kaplowitz
Impaired NO signaling in small pulmonary arteries of chronically hypoxic newborn piglets
Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1244 - L1254.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. L. Conhaim, K. E. Watson, D. M. Heisey, G. E. Leverson, and B. A. Harms
Thromboxane receptor analog, U-46619, redistributes pulmonary microvascular perfusion in isolated rat lungs
J Appl Physiol, January 1, 2004; 96(1): 245 - 252.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online