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Am J Physiol Lung Cell Mol Physiol 284: L376-L385, 2003. First published November 15, 2002; doi:10.1152/ajplung.00218.2002
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Vol. 284, Issue 2, L376-L385, February 2003

Modulation of sodium transport in fetal alveolar epithelial cells by oxygen and corticosterone

Ulrich H. Thome1, Ian C. Davis2, Susie Vo Nguyen2, Brent Jay Shelton3, and Sadis Matalon1,2

1 Departments of Pediatrics, 2 Anesthesiology, and 3 Biostatistics, University of Alabama at Birmingham, Birmingham Alabama 35226

Regulation of active Na+ transport across fetal distal lung epithelial cells (FDLE) by corticosterone (CST), corticotropin-releasing hormone (CRH), and oxygen tension may be crucial for postnatal adaptation. FDLE isolated from 19-day rat fetuses (term: 22 days) were grown on permeable supports to confluent monolayers (duration 3 days) in 2.5, 5, 12, or 20% O2 with 5% CO2-balance N2 and mounted in Ussing chambers for measurement of short-circuit currents (Isc). FDLE monolayers grown in 20% O2 had significantly higher levels of total Isc and of their amiloride-sensitive (Iamil) and ouabain-sensitive (Iouab) components than hypoxic cells. Values (µA/cm2 ± SE) for 2.5-5% O2 and 20% O2 were, respectively, Isc 5.3 ± 0.2 vs. 8.4 ± 0.3 (P < 0.001), Iamil 3.4 ± 0.2 vs. 4.3 ± 0.2 (P < 0.01), and Iouab 3.4 ± 0.6 vs. 9.1 ± 0.6 (P < 0.001). Addition of CST but not CRH to the culture medium at any O2 concentration increased Iamil. FDLE cells grown at 5% O2 expressed significantly lower levels of alpha -, beta -, and gamma -epithelial Na+ channel (ENaC), and of the alpha 1-Na+-K+-ATPase, as determined by Western blotting. We conclude that higher O2 concentrations increased total vectorial Na+ transport, and the function of Na+-K+-ATPase and apical amiloride-sensitive Na+ conductance, whereas CST only increased ENaC function.

Ussing chamber; short-circuit current; sodium-potassium-adenosinetriphosphatase; epithelial sodium channel; oxygen; corticosterone; corticotropin-releasing hormone


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