AJP - Lung AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 284: L882-L890, 2003. First published December 13, 2002; doi:10.1152/ajplung.00211.2002
1040-0605/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/5/L882    most recent
00211.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Numanami, H.
Right arrow Articles by Robbins, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Numanami, H.
Right arrow Articles by Robbins, R. A.
Vol. 284, Issue 5, L882-L890, May 2003

Serine protease inhibitors modulate chemotactic cytokine production by human lung fibroblasts in vitro

Hiroki Numanami1, Sekiya Koyama2, Esturo Sato2, Masayuki Haniuda3, Dan K. Nelson1, Jeffrey C. Hoyt1, Jon L. Freels1, Michael P. Habib1, and Richard A. Robbins1

1 Research Service, Southern Arizona Veterans Health Care System, and Arizona Respiratory Center, University of Arizona, Tucson, Arizona 85723; and 2 The First Department of Internal Medicine and 3 The Second Department of Surgery, School of Medicine, Shinshu University, Matsumoto, Japan 390-0802

Chemotactic chemokines can be released from lung fibroblasts in response to interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha . An imbalance between proteases and antiproteases has been observed at inflammatory sites, and, therefore, protease inhibitors might modulate fibroblast release of chemotactic cytokines. To test this hypothesis, serine protease inhibitors (FK-706, alpha 1-antitrypsin, or Nalpha -p-tosyl-L-lysine chloromethyl ketone) were evaluated for their capacity to attenuate the release of neutrophil chemotactic activity (NCA) or monocyte chemotactic activity (MCA) from human fetal lung fibroblasts (HFL-1). Similarly, the release of the chemoattractants IL-8, granulocyte colony-stimulating factor, monocyte chemoattractant protein-1, macrophage colony-stimulating factor, and granulocyte/macrophage colony-stimulating factor, from HFL-1, were evaluated in response to IL-1beta and TNF-alpha . NCA, MCA, and chemotactic cytokines were attenuated by FK-706. However, matrix metalloproteinase inhibitors were without effect, and cysteine protease inhibitors only slightly attenuated chemotactic or cytokine release. These data suggest that IL-1beta and TNF-alpha may stimulate lung fibroblasts to release NCA and MCA by a protease-dependent mechanism and that serine protease inhibitors may attenuate the release.

neutrophil; monocyte; interleukin-8; monocyte chemoattractant protein-1; granulocyte/macrophage colony- stimulating factor


This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. Paul-Clark, S. K. McMaster, R. Sorrentino, S. Sriskandan, L. K. Bailey, L. Moreno, B. Ryffel, V. F. Quesniaux, and J. A. Mitchell
Toll-like Receptor 2 Is Essential for the Sensing of Oxidants during Inflammation
Am. J. Respir. Crit. Care Med., February 15, 2009; 179(4): 299 - 306.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. D. Bland, R. Ertsey, L. M. Mokres, L. Xu, B. E. Jacobson, S. Jiang, C. M. Alvira, M. Rabinovitch, E. S. Shinwell, and A. Dixit
Mechanical ventilation uncouples synthesis and assembly of elastin and increases apoptosis in lungs of newborn mice.: Prelude to defective alveolar septation during lung development?
Am J Physiol Lung Cell Mol Physiol, January 1, 2008; 294(1): L3 - L14.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online