AJP - Lung Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 284: L917-L925, 2003. First published January 10, 2003; doi:10.1152/ajplung.00374.2002
1040-0605/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/6/L917    most recent
00374.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gao, B.
Right arrow Articles by McCord, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gao, B.
Right arrow Articles by McCord, J. M.
Vol. 284, Issue 6, L917-L925, June 2003

EDITORIAL FOCUS
Synthesis and anti-inflammatory activity of a chimeric recombinant superoxide dismutase: SOD2/3

Bifeng Gao, Sonia C. Flores, Jonathan A. Leff, Swapan K. Bose, and Joe M. McCord

Webb-Waring Institute, University of Colorado Health Sciences Center, Denver, Colorado 80262

External surfaces of cells are normally protected by extracellular superoxide dismutase, SOD3, which binds to polyanions such as heparan sulfate. We constructed a fusion gene encoding a chimeric SOD consisting of the mature human mitochondrial SOD2 plus the COOH-terminal 26-amino acid heparin-binding "tail" from SOD3. This tail is responsible for the enzyme's affinity for endothelial surfaces. The fusion gene was expressed in Escherichia coli, and the fully active enzyme SOD2/3 was purified. Although native SOD2 has no affinity for heparin, SOD2/3 binds to a heparin-agarose column. In a rat model of acute lung injury induced by intratracheal instillation of IL-1, SOD2/3, SOD2, and denatured SOD2/3 showed 92%, 13.8%, and 0% reduction of lung leak, respectively. Only SOD2/3 prevented neutrophil accumulation. In the carrageenan-induced foot edema model in the rat, SOD2/3 reduced edema by 62% (P < 0.003) at a dose in which native SOD2 produced no significant effect. Thus SOD2/3 appears to have properties as a therapeutic anti-inflammatory agent that are greatly superior to other available forms of the enzyme.

inflammation; lung injury; interleukin-1; free radicals; oxidative injury


This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
V. V. Shuvaev, M. Christofidou-Solomidou, F. Bhora, K. Laude, H. Cai, S. Dikalov, E. Arguiri, C. C. Solomides, S. M. Albelda, D. G. Harrison, et al.
Targeted Detoxification of Selected Reactive Oxygen Species in the Vascular Endothelium
J. Pharmacol. Exp. Ther., November 1, 2009; 331(2): 404 - 411.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Muro, M. Mateescu, C. Gajewski, M. Robinson, V. R. Muzykantov, and M. Koval
Control of intracellular trafficking of ICAM-1-targeted nanocarriers by endothelial Na+/H+ exchanger proteins
Am J Physiol Lung Cell Mol Physiol, May 1, 2006; 290(5): L809 - L817.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
Y. Chu, A. Alwahdani, S. Iida, D. D. Lund, F. M. Faraci, and D. D. Heistad
Vascular Effects of the Human Extracellular Superoxide Dismutase R213G Variant
Circulation, August 16, 2005; 112(7): 1047 - 1053.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. P. Bowler, M. Nicks, K. Tran, G. Tanner, L.-Y. Chang, S. K. Young, and G. S. Worthen
Extracellular Superoxide Dismutase Attenuates Lipopolysaccharide-Induced Neutrophilic Inflammation
Am. J. Respir. Cell Mol. Biol., October 1, 2004; 31(4): 432 - 439.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. S. Bonder, D. Knight, D. Hernandez-Saavedra, J. M. McCord, and P. Kubes
Chimeric SOD2/3 inhibits at the endothelial-neutrophil interface to limit vascular dysfunction in ischemia-reperfusion
Am J Physiol Gastrointest Liver Physiol, September 1, 2004; 287(3): G676 - G684.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Gow and H. Ischiropoulos
Super-SOD: superoxide dismutase chimera fights off inflammation
Am J Physiol Lung Cell Mol Physiol, June 1, 2003; 284(6): L915 - L916.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.