HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/4/L931    most recent 00175.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (13) Citing Articles via Google Scholar Google Scholar Articles by Evans, M. J. Articles by Plopper, C. G. Search for Related Content PubMed PubMed Citation Articles by Evans, M. J. Articles by Plopper, C. G.

Atypical development of the tracheal basement membrane zone of infant rhesus monkeys exposed to ozone and allergen

¹Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, Center for Comparative Respiratory Biology and Medicine, University of California, Davis, California 95616; and ²Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606

Submitted 30 May 2003 ; accepted in final form 19 June 2003

Development of the basement membrane zone (BMZ) occurs postnatally in the rhesus monkey. The purpose of this study was to determine whether house dust mite allergen (HDMA) plus ozone altered this process. Rhesus monkeys were exposed to a regimen of HDMA and/or ozone or filtered air for 6 mo. To detect structural changes in the BMZ, we measured immunoreactivity of collagen I. To detect functional changes in the BMZ, we measured perlecan and fibroblast growth factor-2 (FGF-2). We also measured components of the FGF-2 ternary signaling complex [fibroblast growth factor receptor-1 (FGFR-1) and syndecan-4]. The width of the BMZ was irregular in the ozone groups, suggesting atypical development of the BMZ. Perlecan was also absent from the BMZ. In the absence of perlecan, FGF-2 was not bound to the BMZ. However, FGF-2 immunoreactivity was present in basal cells, the lateral intercellular space (LIS), and attenuated fibroblasts. FGFR-1 immunoreactivity was downregulated, and syndecan-4 immunoreactivity was upregulated in the basal cells. This suggests that FGF-2 in basal cells and LIS may be bound to the syndecan-4. We conclude that ozone and HDMA plus ozone effected incorporation of perlecan into the BMZ, resulting in atypical development of the BMZ. These changes are associated with specific alterations in the regulation of FGF-2, FGFR-1, and syndecan-4 in the airway epithelial-mesenchymal trophic unit, which may be associated with the developmental problems of lungs associated with exposure to ozone.

perlecan; fibroblast growth factor-2; fibroblast growth factor receptor-1; syndecan-4; epithelial-mesenchymal trophic unit

This article has been cited by other articles:

				D. M. Hyde, L. A. Miller, E. S. Schelegle, M. V. Fanucchi, L. S. Van Winkle, N. K. Tyler, M. V. Avdalovic, M. J. Evans, R. Kajekar, A. R. Buckpitt, et al. Asthma: a comparison of animal models using stereological methods Eur. Respir. Rev., December 1, 2006; 15(101): 122 - 135. [Abstract] [Full Text] [PDF]

				M. V. Fanucchi, C. G. Plopper, M. J. Evans, D. M. Hyde, L. S. Van Winkle, L. J. Gershwin, and E. S. Schelegle Cyclic exposure to ozone alters distal airway development in infant rhesus monkeys Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L644 - L650. [Abstract] [Full Text] [PDF]

				M. R. Van Scott, D. Aycock, E. Cozzi, K. Salleng, and H. W. Stallings III Separation of bronchoconstriction from increased ventilatory drive in a nonhuman primate model of chronic allergic asthma J Appl Physiol, December 1, 2005; 99(6): 2080 - 2086. [Abstract] [Full Text] [PDF]