AJP - Lung Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 285: L1087-L1098, 2003. First published July 18, 2003; doi:10.1152/ajplung.00409.2002
1040-0605/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/5/L1087    most recent
00409.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Singer, C. A.
Right arrow Articles by Gerthoffer, W. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Singer, C. A.
Right arrow Articles by Gerthoffer, W. T.

p38 MAPK and NF-{kappa}B mediate COX-2 expression in human airway myocytes

Cherie A. Singer,1 Kimberly J. Baker,2 Alan McCaffrey,1 David P. AuCoin,2 Melissa A. Dechert,2 and William T. Gerthoffer1,2

1Department of Pharmacology and 2Cell and Molecular Biology Program, University of Nevada School of Medicine, Reno, Nevada 89557-0046

Submitted 27 November 2002 ; accepted in final form 17 July 2003

We have previously demonstrated that p38 and extracellular signal-regulated protein kinase (ERK) mitogen-activated protein kinases (MAPK) are components of proinflammatory induced cytokine expression in human airway myocytes. The experiments described here further these studies by examining p38 MAPK and NF-{kappa}B regulation of cyclooxygenase-2 (COX-2) expression in response to a complex inflammatory stimulus consisting of 10 ng/ml interleukin (IL)-1{beta}, tumor necrosis factor-{alpha} (TNF-{alpha}), and interferon (IFN)-{gamma}. COX-2 expression was induced with this stimulus in a time-dependent manner, with maximal expression seen 12-20 h after treatment. Semiquantitative RT-PCR and immunoblotting experiments demonstrate decreased COX-2 expression following treatment with the p38 MAPK inhibitor SB-203580 (25 µM) or the proteosome inhibitor MG-132 (1 µM). SB-203580 did not affect cytokine-stimulated I{kappa}B{alpha} degradation, NF-{kappa}B nuclear binding activity, or NF-{kappa}B-dependent signaling from the COX-2 promoter, indicating that p38 MAPK and NF-{kappa}B may affect COX-2 expression via separate signaling pathways. SB-203580, but not MG-132, also increased the initial rate of COX-2 mRNA decay, indicating p38 MAPK, but not NF-{kappa}B, participates in the regulation of COX-2 mRNA stability. These findings suggest that although p38 MAPK and NF-{kappa}B signaling regulate steady-state levels of COX-2 expression, p38 MAPK additionally affects stability of COX-2 mRNA in cytokine-stimulated human airway myocytes.

inflammation; mitogen-activated protein kinase; mRNA stability; cytokines


Address for reprint requests and other correspondence: C. A. Singer, Dept. of Pharmacology/318, Univ. of Nevada School of Medicine, Reno, NV 89557-0046 (E-mail: cas{at}med.unr.edu).




This article has been cited by other articles:


Home page
 Proc Am Thorac SocHome page
O. Tliba and Y. Amrani
Airway Smooth Muscle Cell as an Inflammatory Cell: Lessons Learned from Interferon Signaling Pathways
Proceedings of the ATS, January 1, 2008; 5(1): 106 - 112.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
Y. T.Y. Li, K. E. Swales, G. J. Thomas, T. D. Warner, and D. Bishop-Bailey
Farnesoid X Receptor Ligands Inhibit Vascular Smooth Muscle Cell Inflammation and Migration
Arterioscler. Thromb. Vasc. Biol., December 1, 2007; 27(12): 2606 - 2611.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
H. F. Kramer and L. J. Goodyear
Exercise, MAPK, and NF-{kappa}B signaling in skeletal muscle
J Appl Physiol, July 1, 2007; 103(1): 388 - 395.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Kanefsky, M. Lenburg, and C.-M. Hai
Cholinergic Receptor and Cyclic Stretch-Mediated Inflammatory Gene Expression in Intact ASM
Am. J. Respir. Cell Mol. Biol., April 1, 2006; 34(4): 417 - 425.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. M. Pascual, E. M. Carr, M. C. Seeds, M. Guo, R. A. Panettieri Jr., S. P. Peters, and R. B. Penn
Regulatory features of interleukin-1beta-mediated prostaglandin E2 synthesis in airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L501 - L508.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Wahl, T. J. Eddinger, and C.-M. Hai
Sinusoidal length oscillation- and receptor-mediated mRNA expression of myosin isoforms and {alpha}-SM actin in airway smooth muscle
Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1697 - C1708.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
A. K. Mandal, Z. Zhang, R. Ray, M. S. Choi, B. Chowdhury, N. Pattabiraman, and A. B. Mukherjee
Uteroglobin Represses Allergen-induced Inflammatory Response by Blocking PGD2 Receptor-mediated Functions
J. Exp. Med., May 17, 2004; 199(10): 1317 - 1330.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. Y. Park, M. Joo, T. Pedchenko, T. S. Blackwell, and J. W. Christman
Regulation of macrophage cyclooxygenase-2 gene expression by modifications of histone H3
Am J Physiol Lung Cell Mol Physiol, May 1, 2004; 286(5): L956 - L962.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.