AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 285: L1166-L1178, 2003. First published August 8, 2003; doi:10.1152/ajplung.00182.2003
1040-0605/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/5/L1166    most recent
00182.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (46)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coyne, C. B.
Right arrow Articles by Johnson, L. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coyne, C. B.
Right arrow Articles by Johnson, L. G.

Role of claudin interactions in airway tight junctional permeability

Carolyn B. Coyne,1,2 Todd M. Gambling,3 Richard C. Boucher,1,4 Johnny L. Carson,1,3,4 and Larry G. Johnson1,2,4

1Cystic Fibrosis/Pulmonary Research and Treatment Center, Departments of 2Pharmacology and 4Medicine, and 3Center for Environmental Medicine and Lung Biology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599

Submitted 6 June 2003 ; accepted in final form 3 August 2003

Airway epithelial tight junctions (TJs) serve to separate the external and internal environments of the lung. However, the members of the claudin family that mediate this function have not been fully delineated. We characterized the claudin expression in normal airways removed from human donors during lung transplantation and determined the contribution of each claudin to airway barrier function. Stable cell lines in NIH/3T3 and human airway (IB3.1) cells were constructed expressing the claudin components found in the human airway, claudin-1, -3, or -5. The effects of claudin expression on transepithelial resistance, permeability coefficients, and claudin-claudin interactions were assessed. Claudin-1 and -3 decreased solute permeability, whereas claudin-5 increased permeability. We also detected oligomerization of claudin-5 in cell lines and in freshly excised human airways. Coimmunoprecipitation studies revealed heterophilic interactions between claudin species in both cell lines and human airway epithelium. These suggest that airway TJs are regulated by claudinclaudin interactions that confer the selectivity of the junction.

tight junction; perfluorooctanoic acid; freeze-fracture


Address for reprint requests and other correspondence: L. G. Johnson, Cystic Fibrosis/Pulmonary Research and Treatment Center, 7123A Thurston Bowles Bldg., CB#7248, UNC-CH, Chapel Hill, NC 27599-7248 (E-mail: Larry_Johnson{at}med.unc.edu).




This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
T. A. Brooks, N. Nametz, R. Charles, and T. P. Davis
Diclofenac Attenuates the Regional Effect of {lambda}-Carrageenan on Blood-Brain Barrier Function and Cytoarchitecture
J. Pharmacol. Exp. Ther., May 1, 2008; 325(2): 665 - 673.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Bagherie-Lachidan, S. I. Wright, and S. P. Kelly
Claudin-3 tight junction proteins in Tetraodon nigroviridis: cloning, tissue-specific expression, and a role in hydromineral balance
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1638 - R1647.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
R. J. Mrsny, G. T. Brown, K. Gerner-Smidt, A. G. Buret, J. B. Meddings, C. Quan, M. Koval, and A. Nusrat
A Key Claudin Extracellular Loop Domain is Critical for Epithelial Barrier Integrity
Am. J. Pathol., April 1, 2008; 172(4): 905 - 915.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. Piontek, L. Winkler, H. Wolburg, S. L. Muller, N. Zuleger, C. Piehl, B. Wiesner, G. Krause, and I. E. Blasig
Formation of tight junction: determinants of homophilic interaction between classic claudins
FASEB J, January 1, 2008; 22(1): 146 - 158.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. L. Daugherty, C. Ward, T. Smith, J. D. Ritzenthaler, and M. Koval
Regulation of Heterotypic Claudin Compatibility
J. Biol. Chem., October 12, 2007; 282(41): 30005 - 30013.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. M. Shasby
Cell-cell adhesion in lung endothelium
Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L593 - L607.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
G. B. Pier and L. Zulianello
Airway Epithelial (Nasal) Cell Monolayers Used To Study Pseudomonas aeruginosa Invasion Are Hyperpolarized and Not Representative of the Human Airway Epithelium
Infect. Immun., December 1, 2006; 74(12): 7043 - 7044.
[Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
E. Puchelle, J.-M. Zahm, J.-M. Tournier, and C. Coraux
Airway Epithelial Repair, Regeneration, and Remodeling after Injury in Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, November 1, 2006; 3(8): 726 - 733.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
L. Gonzalez-Mariscal, M. Del CarmenNamorado, D. Martin, G. Sierra, and J. L. Reyes
The tight junction proteins claudin-7 and -8 display a different subcellular localization at Henle's loops and collecting ducts of rabbit kidney
Nephrol. Dial. Transplant., September 1, 2006; 21(9): 2391 - 2398.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
H. Fujita, H. Chiba, H. Yokozaki, N. Sakai, K. Sugimoto, T. Wada, T. Kojima, T. Yamashita, and N. Sawada
Differential Expression and Subcellular Localization of Claudin-7, -8, -12, -13, and -15 Along the Mouse Intestine
J. Histochem. Cytochem., August 1, 2006; 54(8): 933 - 944.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
Y Soini, V Kinnula, K Kahlos, and P Paakko
Claudins in differential diagnosis between mesothelioma and metastatic adenocarcinoma of the pleura.
J. Clin. Pathol., March 1, 2006; 59(3): 250 - 254.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. A. Brooks, B. T. Hawkins, J. D. Huber, R. D. Egleton, and T. P. Davis
Chronic inflammatory pain leads to increased blood-brain barrier permeability and tight junction protein alterations
Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H738 - H743.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. D. Alexandre, Q. Lu, and Y.-H. Chen
Overexpression of claudin-7 decreases the paracellular Cl- conductance and increases the paracellular Na+ conductance in LLC-PK1 cells
J. Cell Sci., June 15, 2005; 118(12): 2683 - 2693.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. S. L. Yu, K. M. McCarthy, S. A. Francis, J. M. McCormack, J. Lai, R. A. Rogers, R. D. Lynch, and E. E. Schneeberger
Knockdown of occludin expression leads to diverse phenotypic alterations in epithelial cells
Am J Physiol Cell Physiol, June 1, 2005; 288(6): C1231 - C1241.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. M. Van Itallie, T. M. Gambling, J. L. Carson, and J. M. Anderson
Palmitoylation of claudins is required for efficient tight-junction localization
J. Cell Sci., April 1, 2005; 118(7): 1427 - 1436.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. L. Daugherty, M. Mateescu, A. S. Patel, K. Wade, S. Kimura, L. W. Gonzales, S. Guttentag, P. L. Ballard, and M. Koval
Developmental regulation of claudin localization by fetal alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1266 - L1273.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H. Wen, D. D. Watry, M. C. G. Marcondes, and H. S. Fox
Selective Decrease in Paracellular Conductance of Tight Junctions: Role of the First Extracellular Domain of Claudin-5
Mol. Cell. Biol., October 1, 2004; 24(19): 8408 - 8417.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Boitano, Z. Safdar, D. G. Welsh, J. Bhattacharya, and M. Koval
Cell-cell interactions in regulating lung function
Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L455 - L459.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
W. Y. Li, C. L. Huey, and A. S. L. Yu
Expression of claudin-7 and -8 along the mouse nephron
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1063 - F1071.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. Turksen and T.-C. Troy
Barriers built on claudins
J. Cell Sci., May 15, 2004; 117(12): 2435 - 2447.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.