HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Dubin, R. F. Articles by Widdicombe, J. H. Search for Related Content PubMed PubMed Citation Articles by Dubin, R. F. Articles by Widdicombe, J. H.

Secretion of lactoferrin and lysozyme by cultures of human airway epithelium

¹Children's Hospital of Oakland Research Institute, Oakland 94609; and ²Department of Human Physiology, University of California-Davis, Davis, California 95616

Submitted 12 September 2003 ; accepted in final form 3 November 2003

Lactoferrin and lysozyme are important antimicrobial compounds of airway surface liquid, derived predominantly from serous cells of submucosal glands but also from surface epithelium. Here we compared release of these compounds from the following human cell cultures: primary cultures of tracheal epithelium (HTE), Calu-3 cells (a lung adenocarcinoma cell line frequently used as a model of serous gland cells), 16HBE14o^- cells (an SV40 transformed line from airway surface epithelium), T84 cells (a colon carcinoma cell line), and human foreskin fibroblasts (HFF). For lysozyme, baseline secretory rates were in the order Calu-3 > 16HBE14o^- > HTE T84 > HFF = 0; for lactoferrin, the only cell type showing measurable release was HTE; for mucus, HTE > Calu-3 > 16HBE14o^- T84 > HFF = 0. A wide variety of neurohumoral agents and inflammatory stimuli was without effect on lactoferrin and lysozyme release from HTE or Calu-3 cells, although forskolin did stimulate secretion of water and lysozyme from Calu-3 cells. However, the concentration of lysozyme in the forskolin-induced secretions was much less than in airway gland secretions. Thus our data cast doubt on the utility of Calu-3 cells as a model of airway serous gland cells but do suggest that HTE could prove highly suitable for studies of mucin synthesis and release.

mucin; airway surface liquid; serous gland cells

This article has been cited by other articles:

				S. M. Kreda, S. F. Okada, C. A. van Heusden, W. O'Neal, S. Gabriel, L. Abdullah, C. W. Davis, R. C. Boucher, and E. R. Lazarowski Coordinated release of nucleotides and mucin from human airway epithelial Calu-3 cells J. Physiol., October 1, 2007; 584(1): 245 - 259. [Abstract] [Full Text] [PDF]

				R. J. Lee, M. P. Limberis, M. F. Hennessy, J. M. Wilson, and J. K. Foskett Optical imaging of Ca2+-evoked fluid secretion by murine nasal submucosal gland serous acinar cells J. Physiol., August 1, 2007; 582(3): 1099 - 1124. [Abstract] [Full Text] [PDF]

				B. Grubor, D. K. Meyerholz, and M. R. Ackermann Collectins and cationic antimicrobial peptides of the respiratory epithelia. Vet. Pathol., September 1, 2006; 43(5): 595 - 612. [Abstract] [Full Text] [PDF]

				J. A. Nash, T. N. S. Ballard, T. E. Weaver, and H. T. Akinbi The Peptidoglycan-Degrading Property of Lysozyme Is Not Required for Bactericidal Activity In Vivo J. Immunol., July 1, 2006; 177(1): 519 - 526. [Abstract] [Full Text] [PDF]

				R. Dajani, Y. Zhang, P. J. Taft, S. M. Travis, T. D. Starner, A. Olsen, J. Zabner, M. J. Welsh, and J. F. Engelhardt Lysozyme Secretion by Submucosal Glands Protects the Airway from Bacterial Infection Am. J. Respir. Cell Mol. Biol., June 1, 2005; 32(6): 548 - 552. [Abstract] [Full Text] [PDF]