HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 286/4/L756    most recent 00214.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Ye, X. Articles by Folkesson, H. G. Search for Related Content PubMed PubMed Citation Articles by Ye, X. Articles by Folkesson, H. G.

IL-1 stimulates alveolar fluid absorption in fetal guinea pig lungs via the hypothalamus-pituitary-adrenal gland axis

Department of Physiology and Pharmacology, Northeastern Ohio Universities College of Medicine, Rootstown, Ohio 44272-0095

Submitted 3 July 2003 ; accepted in final form 23 November 2003

We tested the hypothesis that interleukin (IL)-1-induced cortisol synthesis stimulates alveolar fluid clearance in preterm fetuses. IL-1 was administered subcutaneously daily to timed-pregnant guinea pigs for 3 days with and without simultaneous cortisol synthesis inhibition by metyrapone. Fetuses were obtained by abdominal hysterotomy at 61 and 68 days gestation and instilled with isosmolar 5% albumin in the lungs, and alveolar fluid movement was measured over 1 h from the change in alveolar protein concentration. Alveolar fluid clearance was induced at 61 days gestation and stimulated at 68 days gestation by IL-1, which both were attenuated by cortisol synthesis inhibition. Plasma ACTH and cortisol concentrations were increased by IL-1 at both gestational ages, and metyrapone reduced cortisol concentrations. IL-1 was mostly low or undetectable in both fetal and maternal blood. Prenatal alveolar fluid clearance, when present as well as IL-1 induced, was always propranolol and amiloride sensitive, suggesting that -adrenoceptor stimulation and amiloride-sensitive Na⁺ channels were critical for fluid absorption. IL-1 increased lung -adrenoceptor density at gestation day 61, and cortisol synthesis inhibition attenuated the increased -adrenoceptor density. Epithelial Na⁺ channel and Na⁺-K⁺-ATPase subunit expressions were both increased by IL-1 and attenuated by cortisol synthesis inhibition. These results may explain why babies delivered preterm after intrauterine inflammation have a reduced risk of developing severe respiratory distress.

adrenocorticotropic hormone; alveolar epithelium; -adrenoceptors; cortisol; epithelial sodium channel; epinephrine; interleukin-1; respiratory distress syndrome; prenatal lung development; sodium transport

This article has been cited by other articles:

				T. Li, S. Koshy, and H. G. Folkesson IL-1{beta}-induced cortisol stimulates lung fluid absorption in fetal guinea pigs via SGK-mediated Nedd4-2 inhibition Am J Physiol Lung Cell Mol Physiol, March 1, 2009; 296(3): L527 - L533. [Abstract] [Full Text] [PDF]

				T. Li, S. Koshy, and H. G. Folkesson Involvement of {alpha}ENaC and Nedd4-2 in the conversion from lung fluid secretion to fluid absorption at birth in the rat as assayed by RNA interference analysis Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L1069 - L1078. [Abstract] [Full Text] [PDF]

				T. Li and H. G. Folkesson RNA interference for {alpha}-ENaC inhibits rat lung fluid absorption in vivo Am J Physiol Lung Cell Mol Physiol, April 1, 2006; 290(4): L649 - L660. [Abstract] [Full Text] [PDF]

				P. K. Nair, T. Li, R. Bhattacharjee, X. Ye, and H. G. Folkesson Oxytocin-induced labor augments IL-1{beta}-stimulated lung fluid absorption in fetal guinea pig lungs Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L1029 - L1038. [Abstract] [Full Text] [PDF]